NOTEBOOK

PCR

Collects of blood samples in patients treated at Mastology service of Baron de Lucena Hospital-HBL, data on the patients as: socio-cultural aspects (name, place of residence, occupation, parity, smoking) and clinical (age at diagnosis, histological type, histological grade, tumor side, tumor size, and family history) collected from medical records and laboratorial reports of their patients were recorded in appropriate forms.

Procedure for collection of biological samples:

• 1) The patients signed a consent form, where all procedures contained in this research concerned.
• 2) 5ml blood of patients with breast cancer were collected in EDTA tubes containing 2 ml of RNAlater.
• 3) After collecting tubes containing blood of patients are referred to the laboratory for RNA extraction.