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Team:Cornell/notebook/plasmids
From 2014.igem.org
Plasmids
| Plasmid Name | Part | Contents/Description | Origin | Backbone | Antibiotic Resistance |
|---|---|---|---|---|---|
| pA14A | pDH1 | mer operon | Wilson | pCL1921 | Ampicillin |
| pA14B | pGYMT | GST-YMT | Wilson | pGEX-2T | Ampicillin |
| pA14C | pUEF202 | nixA | Wilson | pBluescript | Ampicillin |
| pA14D | pGPMT | GST-PMT | Wilson | pGPMT | Ampicillin |
| pAK14E | BBa_I712074 | T7 promoter | pAK13D | pSB1AK8 | Ampicillin |
| pA14F | BBa_J23102 | Anderson Promoter+mRFP | 2013 KP5: 18G | pJ61002 | Ampicillin |
| pA14G | invalid | A-Promoter+RBS+merT+merP | A+F | pJ61002 | Ampicillin |
| pC14H | BBa_J04450 | mRFP in pSB1C3 | pC13F | pSB1C3 | Chloramphenicol |
| pC14I | not submitted | A-Promoter+RBS+merT+merP+ter BioBrick | AG+H | pSB1C3 | Chloramphenicol |
| pA14J | invalid | A-Promoter+RBS+nixA | C+F | pJ61002 | Ampicillin |
| pC14K | not submitted | A-Promoter+RBS+nixA+ter BioBrick | AF+T | pSB1C3 | Chloramphenicol |
| pAK14L | invalid | T7+GST-YMT | B+E | pSB1AK8 | Ampicillin |
| pC14M | not submitted | T7+GST-YMT+ter in pSB1C3 | L+T | pSB1C3 | Chloramphenicol |
| pAK14N | invalid | T7+GST-PMT | D+E | pSB1AK8 | Ampicillin |
| pC14O | not submitted | T7+GST-PMT+ter | N+T | pSB1C3 | Chloramphenicol |
| pC14P | not submitted | T7+GST-YMT BioBrick | M | pSB1C3 | Chloramphenicol |
| pC14Q | not submitted | T7+GST-PMT BioBrick | O | pSB1C3 | Chloramphenicol |
| pA14R | invalid | A-Promoter+RBS+ CBP4 + Biobrick prefix and suffix | BioBasic | pUC57 | Ampicillin |
| pC14S | not submitted | A-promoter+RBS+CBP4 BioBrick | R+H | pSB1C3 | Chloramphenicol |
| pC14T | BBa_B1006 | Terminator | 2013 KP: 6D | pSB1C3 | Chloramphenicol |
| pC14U | not submitted | Final mercury + GST-YMT construct | I+P | pSB1C3 | Chloramphenicol |
| pC14V | not submitted | Final nickel + GST-YMT construct | K+P | pSB1C3 | Chloramphenicol |
| pC14W | not submitted | Final lead + GST-YMT construct | S+P | pSB1C3 | Chloramphenicol |
| pC14X | not submitted | Final mercury + GST-PMT construct | I+Q | pSB1C3 | Chloramphenicol |
| pC14Y | not submitted | Final nickel + GST-PMT construct | K+Q | pSB1C3 | Chloramphenicol |
| pC14Z | not submitted | Final lead + GST-PMT construct | S+Q | pSB1C3 | Chloramphenicol |
| pC14AA | BBa_K540001 | Nickel inducible | Kit Plate | pSB1C3 | Chloramphenicol |
| pC14AB | BBa_K346002 | mercury inducible | Kit Plate | pSB1C3 | Chloramphenicol |
| pC14AC | BBa_K206000 | pBAD | 2014 KP3: 14A | pSB1C3 | Chloramphenicol |
| pC14AD | not submitted | Nickel inducible + mRFP | AA+H | pSB1C3 | Chloramphenicol |
| pC14AE | not submitted | Mercury inducible + mRFP | AB+H | pSB1C3 | Chloramphenicol |
| pA14AF | invalid | NixA Synthesized (A-promoter, RBS, ter) | GenScript | pUC57 | Ampicillin |
| pA14AG | invalid | MerT/MerP Synthesized (A-Promoter, RBS, ter) | GenScript | pUC57 | Ampicillin |
| pA14AH | invalid | GST-YMT Synthesized (pT7, RBS, ter) | GenScript | pUC57 | Ampicillin |
| pC14AI | not submitted | GST-YMT Synthesized (pT7, RBS, ter) in pSB1C3 | AH+H | pSB1C3 | Chloramphenicol |
| pC14AJ | BBa_K592009 | amilCP in pSB1C3 | 2014 KP1: 19E | pSB1C3 | Chloramphenicol |
| pC14AK | not submitted | GST-YMT Synthesized without T7 (RBS,ter) in pSB1C3 | AI + T7 | pSB1C3 | Chloramphenicol |
