Two weeks ago we had an email in our inbox that made us very happy: We had won a gene synthesis from [http://eurofinsgenomics.eu/en/eurofins-genomics/corporate-information/deals-promotions/igem-sponsorship.aspx Eurofins Genomics]!
On Wednesday, we were then visited by Ms. Blumstein who handed us the voucher. Then we took this beautiful photo:
Blog #19 - Open Hardware at the Maker Faire Hannover
This weekend Florian, René and Michael joined Ansgar on a trip to the [http://makerfairehannover.com/ Maker Faire Hannover 2014].
The Maker Faire is an exhibition with things that people built on their own using readily available tools. Dozens of 3D printers and microcontrollers can be examined and there are a lot of devices visitors can interact with.
Apart from the fire-spitting dragon, our OD and fluorescence measurement devices attracted a lot of attention. We spoke with hundreds of visitors and had a lot of interesting conversations.
Saturday evening, Carsten Ludwig from the iGEM Team Braunschweig visited us. We showed him the most fascinating stands and afterwards went to the city to walk the [http://www.roterfaden-hannover.de/ Red Thread of Hannover], a track going past 36 very central tourist attractions as e.g. the roofless church:
When the #NEDCRC soccer game started, we settled for some hamburgers, fermentation broth and exchanged funny stories about our lab experience.
On Saturday the run on our stand had been so enormous that we had to improvise and print new flyers at our hostel for Sunday, the second and last day of the fair. Just like the day before, a lot of people showed interest in our stand and especially René passionately demonstrated our devices and informed about synthetic biology as well as our project "Cellock Holmes".
At about midday, Manuel, advisor of Team Bielefeld, visited us and got to experience our measurement device too.
When we left Hannover Monday morning, we decided to stop by in Bielefeld to meet the rest of their team. This way they too got to test our OD and fluorescence measurement device and we got to eat some delicious ribes cake :)
Five hours later, we left the Bielefeld team to eat some incredibly spicy pizza and continue our journey back home to Aachen. On the Autobahn we took the time to write down all experiences and things we learned to share them with our team in the coming days.
tl;dr: We had an awesome trip to the Maker Faire Hannover, presented our devices to hundreds of people and met the teams Braunschweig and Bielefeld.
If you like to see more about our weekend have a look at the following gallery =)
Trying to squeeze everything into the much too small trunk...
Hmm... how does that work?
Finally! Let's go to Hannover!
Getting close to our destination
After a little traffic jam we made it to the MakerFaire
Florian ready to "Make Light"!
Some last preparations...
The Visitation of Smaug
Enthusing future Nobel Prize laureate with Synthetic Biology and Open Hardware
Caught the attention of VDI (The Association of German Engineers)
Am I going to become a TV star?
Trying to hold back stage fright
Carsten from iGEM Team Braunschweig arrived and burns with desire to get to know our devices ;)
Yesterday, RWTH organized an open house for students from ages 14 to 16. Different departments, institutes and student councils presented exhibits, latest research projects, and the many programs they offer. Students could actively try out science and research in prepared experiments at each stand. Various groups of student representatives also offered first hand advice to students with respect to what to study and when and where to collect information. In presentations about the different fields of studies, students gained first insights into the world of academic professions and other career perspectives.
Our iGEM team, too, supported the bioscience department with our own stand. We presented a prototype of our measurement device Cellock Holmes that students could use to measure the fluorescence of different riboflavin solutions. Apart from giving general advice as university students to high school students, Nina and Florian explained students how they can get involved in research outside of a specific university program.
Blog #17 - Noneffective Antibiotics (Part III): Companies are Looking for Microbiologists
Our "Synthetic Biology" teaching module has come to an end. Today was our last day at Kaiser-Karl-Gymnasium in Aachen, where we have worked together with a grade 9 biology-chemistry class for past 1 1/2 months. The students have learned about the iGEM and synthetic biology in general. We presented our project to the class, and explained various aspects for our endeavour in more detail.
To give interested students some overview of how they can get involved in synthetic biology, Vera, Florian, Ansgar and Björn gave short presentations about each university program at RWTH represented in our team: Biology, Biotechnology, Computational Engineering Science and Informatics, respectively. We gave them examples for typical classes and specialization options as well as career perspectives in each program.
While discussing some more career perspectives, we handed an evaluation sheet out to each student. This way the class could give us some feedback about our work, and leave some comments on what they liked best and what we can still improve on. On average, the students gave us an A (1,9 or 87%) for the teaching module. We are really happy about this great mark, and will try to improve on all the things the students suggested.
Unfortunately, the bell already rang when the students finished handing in their evaluation sheets. However, some students still followed our invitation to stay after class to ask more questions about paths into synthetic biology, and for some cookies.
Overall, all parties seemed really satisfied with our collaboration and we are looking forward to repeating the teaching module with other high school classes!
Today Nina and Arne discussed last class's experiment. Arne explained to the students the meaning behind the values measured, and how the students can use the value to determine the concentration of riboflavin in their vanilla pudding samples.
Subsequently, Nina explained the students how we are going to manipulate regular E. coli cells, using synthetic biology methods, to carry out the desired functions. The students also learned, what role promoters play in gene regulation, and how our iGEM team wants to regulate the expression of the genes artificially inserted into the E. coli cells.
Now we have individually discussed all aspects of our project. Therefore, Nina summarized all the topic together with the students, and outlined the link between all these different topics and our iGEM project idea. In doing so, we almost arrived at the end of our teaching module "Synthetic Biology". Next class, we will visit the biology-chemistry course of Kaiser-Karl-Gymnasium for the last time, and do some career-exploration in synthetic biology and related fields.
The topics of today's double lesson was quorum sensing as well as measurement of fluorescence. At the beginning of class, the students form 6 groups and start an experiment dealing with fluorescence: Each group weighs and dissolves 4g of vanilla pudding powder in 50mL of water. While conducting the experiment, each group is supervised by a member of our iGEM team.
While we give the powder some time to dissolve, René discusses the worksheet about quorum sensing that we gave out to the students last week. In doing so, the students learn what quorum sensing actually is, how it is used in different ways by a variety of bacteria, and how we want to use this function for our project.
Meanwhile the powder had dissolved in the water. Excess powder accumulated at the bottom of the beaker. Using a syringe, the students suck 2mL of supernatant out of the beaker, and press it through a filter into a cuvette. A part of our project involves the development of a fluorescence measurement device named "Cellock Holmes". Each group of students at a time places its cuvette in the "Cellock Holmes" prototype, and notes down the values displayed on the cellphone display. Of course, each group also measures the fluorescence of a positive (pure riboflavin in water) and a negative (chalk dust in water) control.
While the other groups wait for their turn, their supervisors explain the background of this experiment: The electrons in some molecules change into energy states when irradiated with electromagnetic waves. When returning to the normal state, the electrons dissipate excess energy, also in the form of electromagnetic radiation. This process is called fluorescence. Vanilla pudding contains the molecule riboflavin (vitamin B2). When riboflavin is irradiated with blue light, the molecule fluoresces green. The intensity of the green light is visually recorded by our device. Special software processes the data and the measured value is displayed on the cellphone connected to the device via Bluetooth. We will discuss the results of the experiment next class.
Last lesson students at Kaiser-Karl-Gymnasium took environmental samples using a variety of different contact agar plates. On the weekend, René examined the plates under the microscope and took photos. Today René and Nina present the students the results of the experiment. But first, we showed the students an excerpt from the TV show "Planetopia" that broaches the issue "Hygiene in every-day life".
Staphylo- and micrococci as well as Enterobacteria, Candida yeasts and miscellaneous fungi are found on the majority of plates. But why is there no growth on the plates containing the antibiotic? Actually, what is an antibiotic, how does it work and why are there multi-resistant pathogens? Nina explained the answer to all these questions to the students using descriptive graphics, and concluded the topic "Microorganisms in our Environment" with a short summmary.
As a starter for our next topic that is relevant to our project, we distribute a worksheet about quorum sensing among the students. For the remainder of the lesson, the students read the text and begin to answer the attached questions.
on the coming weekend 3 of our team (Markus, Michael and Arne) are going to travel to Munich for the iGEM meetup iGEM meets CAS. We are very excited for this and can’t wait to meet all the other teams who will be attending. Stay tuned to this blog to see the first pictures when we come back!
Hello iGEM enthusiasts,
Since our last visit, the student of the biology-chemistry course at Kaiser-Karl-Gymnasium in Aachen have been busy studying the basics of protein biosynthesis and the "lock and key" model. Now the 9th graders are prepared to have a closer look at synthetic biology and our project.
Each year, many people die of nosocominal infections - infections that the patients acquired in a hospital. However, these infections would be preventable in large part through better hygiene programs. Diseases caused by multi-resistant pathogens are especially critical because therapy options are very limited in these cases.
But actually, what are pathogens exactly and where do they come from? In order to answer these questions, we start the teaching module with the topic "Microorganisms in our Environment". In this part, we explain which germs we encounter every day, and how be can best protect ourselves from them.
In order to demonstrate how many and which microorganisms are our steady companions, we conduct an experiment with the students. A pair of students received 3 agar plates: one with regular LB agar, one plate with agar supplemented by an antibiotic, and one selective agar plate for yeasts and fungi. Equipped with the plates, the students wander through their school and take contact samples from places they think many microorganisms grow there. We will incubate the plates over the weekend so that we can show the students in the next lesson what has grown on their samples.
We are currently working together with the Kaiser-Karl-Gymnasium in Aachen. Together we are teaching the students about synthetic biology, iGEM and our project in particular. As a part of this, we are designing cool experiments for the students to do themselves. Here a some nice pictures of us preparing them:
if you are like us and can't wait for our wiki page to go fully online (see last post for more information) and the project to be fully revealed, here are some pictures to tide you over until then:
we are more than happy to finally be able to announce that our application has been finally approved by the iGEM headquarters. Now nothing will stand between us and our journey towards the giant Jamboree at the end of October!
This will bring a few changes towards especially this site. With the approval we now have access to our own wiki website hosted on the iGEM servers. We will migrate all our previous content towards this site in the next weeks. This will include everyhting already posted on here. As soon as we have finished preparing the full migration you will automatically be redirected, so you do not need to remember a different website for all your IGEM Aachen information.
As soon as the migrations has been completed you will also find all the details you need to know about our project we have been teasing about the past weeks on facebook and twitter. So stay tuned!
The project has been progressing nicely in part due to the awesome iGEM Kits provided by NEB:
NEB you are awesome!
Also as always you can follow us on
[http://www.facebook.com/iGEMAachen Facebook]] or [http://twitter.com/igemaachen Twitter]]for more information and a look behind the scenes.
Today was the kick-off for the cooperation between our iGEM team and the Kaiser-Karl-Gymnasium, a secondary school in Aachen.
In the upcoming weeks, exciting school lessons with members of our iGEM team await the students of the 9th grade biology-chemistry class. Different aspects of our project will be highlighted and explained to the students. Demonstrative experiments will explain the practical relevance.
In today's lesson, our members Nina and René offered the students a short impression of what to expect in the course of this teaching module. We also explained synthetic biology and the goal of our project.
Hello everyone,
the iGEM competition has officially started and we are taking part!
If you are as excited as we are stay tuned as we will unveil everything about our project in the upcoming weeks. This blog will function as a behind the scenes look of the project and the team. So stay tuned for everything iGEM Aachen!
In the meantime you can check us out on [http://www.facebook.com/iGEMAachen Facebook] or [http://twitter.com/igemaachen Twitter]
for more updates.
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