Team:Bordeaux/NotebookJune13

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6^th June

Molecular biology

Digestion, ligation and transformation of pSB6A1 and our PCR products VPGXG-20, VPGXG-40 and VPGXG-60.
- Transformation by electroporation in BL21

Purification

End of purification protocol and store at 4°C

Design

Research in bibliography on consensus sequences of dragline silk
- With this sequences, we would like to synthetize our Silk-Like-Polypeptide sequence from our own oligos (based on Gibson assembly protocol)

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