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Team:Bordeaux/NotebookJune13
From 2014.igem.org
13th June
Molecular biology
- Digestion, ligation and transformation of pSB6A1 and our PCR products VPGXG-20, VPGXG-40 and VPGXG-60.
- Transformation by electroporation in BL21
Purification
- End of purification protocol and store at 4°C
Design
- Research in bibliography on consensus sequences of dragline silk
- With this sequences, we would like to synthetize our Silk-Like-Polypeptide sequence from our own oligos (based on Gibson assembly protocol)
