Toggle navigation SCU-Igem Project Background Overview Description Result Modeling Human Practice Presentation at Seventh Senior High School Visiting University of Electronic Science and Technology of China (UESTC) Safety Parts Attributions Team Notebook Notebook Notebook of Biobricks Notebook of Transmitter Notebook of Effector Notebook of Sensor Method Bacterial Genomic DNA Prep Digestion Gel Extraction Linkage PCR Plasmid Mini Prep PCR Protocol Back to top 1. Your Training Have your team members received any safety training yet? Yes, we have already received safety training. Please briefly describe the topics that you learned about (or will learn about) in your safety training. The advisors have given us the safety training such as what risks the chemicals may cause and how to avoid them or reduce the harm as much as possible before the experiment . Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements. Yes, our school has a special department responsible for this part, their officers have inspected our laboratory and gave us suggestions about biosafety. 2. Your Local Rules and Regulations a) Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety, a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion. b) What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link. c) In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link. 3. The Organisms and Parts that You Use Please visit this page to download a blank copy of the spreadsheet for question 3. (If you need a CSV version instead of XLS, visit this page.) Complete the spreadsheet. Include all whole organisms that you will handle in the lab, whether you are using them as a chassis or for some other reason. Include all new or highly modified protein coding parts that you are using. If you submitted a Check-In for an organism or part, you should still include it in this spreadsheet. You may omit non-protein-coding parts, and you may omit parts that were already in the Registry if you are using them without significant modifications. Click here to show/hide instructions for completing the spreadsheet Remember to change the filename of your spreadsheet! Put your team's name in place of "TeamName". Species name (including strain): For an organism, give the scientific name of the species. Include a strain name or number (such as "K-12" for E. coli K-12) if there is one. For a part, give the name and strain of the organism that the part originally came from. Risk Group: Give the Risk Group of the organism in column A. You may use a categorization according to your home country, according to the USA, or according to the WHO. If the organism falls into an 'in-between' or special category such as 2+ or 2-Agricultural, explain this category in the Notes column. If you cannot find any Risk Group categorization for this organism, write "N/A" and explain in the Notes column. (Multicellular organisms generally do not have a Risk Group.) Risk Group Source: Cite the source from which you obtained the Risk Group information. See Risk Group Guide for recommended sources. If you got the information from the Canadian PSDS, from the NIH Guidelines, or from the DSMZ catalogue, you may simply write "PSDS", "NIH", or "DSMZ". Otherwise, please give a web link or a full citation for your source. Disease risk to humans?: Does this organism cause any disease in humans? If yes, what disease does it cause? Part number/name: For a part: If it has a Registry part number (like BBa_XXXXX), write that number. If it has no Registry part number, give a short name for the part. (For example: "Actin", "Alcohol Dehydrogenase".) For a whole organism, leave this column blank. Natural function of part: For a part: Briefly describe what the part does in its parent organism. (If it is an enzyme, what reaction does it catalyze? If it is a receptor, what molecules does it bind to? Etc.) For a whole organism, leave this column blank. How did you acquire it?: Describe how you acquired the organism/part. If you have not acquired it yet, describe how you plan to acquire it. (For example: did you receive the part DNA from another lab? Did you order the part DNA from a synthesis company? Did you use PCR to isolate the part from genomic DNA of its parent organism? Did you order the cell line from a company?) How will you use it?: Describe how you are using the organism/part in the lab. (For example: "This organism is our chassis." "This part senses when the cells are exposed to glucose." "This organism is the source for a part that we are isolating by PCR." "This part produces the toxin which our bio-sensor is designed to detect.") Notes: Use this column to give any additional information that is necessary. Upload Spreadsheet -- Please do not change the "Destination Filename"! You may upload multiple versions of your spreadsheet, using the same Destination Filename. The wiki software will keep track of different versions, and list them in chronological order. Click here to VIEW your spreadsheet 4. Risks of Your Project Now Please describe risks of working with the biological materials (cells, organisms, DNA, etc.) that you are using in your project. If you are taking any safety precautions (even basic ones, like rubber gloves), that is because your work has some risks, however small. Therefore, please discuss possible risks and what you have done (or might do) to minimize them, instead of simply saying that there are no risks at all. a) Risks to the safety and health of team members, or other people working in the lab: b) Risks to the safety and health of the general public (if any biological materials escaped from your lab): c) Risks to the environment (from waste disposal, or from materials escaping from your lab): d) Risks to security through malicious mis-use by individuals, groups, or countries: e) What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.) 5. Risks of Your Project in the Future What would happen if all your dreams came true, and your project grew from a small lab study into a commercial/industrial/medical product that was used by many people? We invite you to speculate broadly and discuss possibilities, rather than providing definite answers. Even if the product is "safe", please discuss possible risks and how they could be addressed, rather than simply saying that there are no risks at all. a) What new risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) Also, what risks might arise if the knowledge you generate or the methods you develop became widely available? b) Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.) Such features are not required for an iGEM project, but many teams choose to explore them. 6. Further Comments If you are completing a Preliminary Version of your Safety Form, use this space to describe how far you have progressed in your project, and give some comments about any questions that you left blank. You can also use this space for any other comments or additional material. Submit Only a team Instructor (or Primary Contact) may submit the Safety Form. Instructors, please read the form you are submitting, and confirm that all its information is correct. By checking the "I Agree" box and clicking the "Submit" button, you are agreeing that the Safety Form accurately describes the activities of your team. We are using the "I Agree" box in lieu of a signature with paper and pen. I Agree We need to go back and edit our answers -- Delete Form (admins only) Sichuan university
PCR Protocol
Yes, we have already received safety training.
The advisors have given us the safety training such as what risks the chemicals may cause and how to avoid them or reduce the harm as much as possible before the experiment .
Yes, our school has a special department responsible for this part, their officers have inspected our laboratory and gave us suggestions about biosafety.
a) Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety, a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.
b) What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.
c) In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.
Please visit this page to download a blank copy of the spreadsheet for question 3. (If you need a CSV version instead of XLS, visit this page.)
Complete the spreadsheet. Include all whole organisms that you will handle in the lab, whether you are using them as a chassis or for some other reason. Include all new or highly modified protein coding parts that you are using. If you submitted a Check-In for an organism or part, you should still include it in this spreadsheet.
You may omit non-protein-coding parts, and you may omit parts that were already in the Registry if you are using them without significant modifications.
Remember to change the filename of your spreadsheet! Put your team's name in place of "TeamName".
Upload Spreadsheet -- Please do not change the "Destination Filename"!
You may upload multiple versions of your spreadsheet, using the same Destination Filename. The wiki software will keep track of different versions, and list them in chronological order.
Click here to VIEW your spreadsheet
Please describe risks of working with the biological materials (cells, organisms, DNA, etc.) that you are using in your project. If you are taking any safety precautions (even basic ones, like rubber gloves), that is because your work has some risks, however small. Therefore, please discuss possible risks and what you have done (or might do) to minimize them, instead of simply saying that there are no risks at all.
a) Risks to the safety and health of team members, or other people working in the lab:
b) Risks to the safety and health of the general public (if any biological materials escaped from your lab):
c) Risks to the environment (from waste disposal, or from materials escaping from your lab):
d) Risks to security through malicious mis-use by individuals, groups, or countries:
e) What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)
What would happen if all your dreams came true, and your project grew from a small lab study into a commercial/industrial/medical product that was used by many people? We invite you to speculate broadly and discuss possibilities, rather than providing definite answers. Even if the product is "safe", please discuss possible risks and how they could be addressed, rather than simply saying that there are no risks at all.
a) What new risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?
b) Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.) Such features are not required for an iGEM project, but many teams choose to explore them.
If you are completing a Preliminary Version of your Safety Form, use this space to describe how far you have progressed in your project, and give some comments about any questions that you left blank.
You can also use this space for any other comments or additional material.
Only a team Instructor (or Primary Contact) may submit the Safety Form.
Instructors, please read the form you are submitting, and confirm that all its information is correct. By checking the "I Agree" box and clicking the "Submit" button, you are agreeing that the Safety Form accurately describes the activities of your team. We are using the "I Agree" box in lieu of a signature with paper and pen.
We need to go back and edit our answers --
Delete Form (admins only)
Sichuan university
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