Harvard BioDesign/25 July 2014

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Sequencing results The stop codon was missing from the ordered oligonucleotide blocks that were used for sequencing Miniprepped 11 Mach I- SpyTag- CsgA colonies growing up from yesterday Sent miniprep product for sequencing Ran a gel to verify the size of the opened up and linearized backbone Ordered new primer/inserts, this time containing the stop codon