"

From 2014.igem.org

Sequencing results The stop codon was missing from the ordered oligonucleotide blocks that were used for sequencing Miniprepped 11 Mach I- SpyTag- CsgA colonies growing up from yesterday Sent miniprep product for sequencing Ran a gel to verify the size of the opened up and linearized backbone Ordered new primer/inserts, this time containing the stop codon