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Team:Hong Kong HKUST/riboregulator/parts
From 2014.igem.org
Riboregulator Parts
Parts that we designed and submitted
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1379015 | RBS | RBS(Lock 3c) | 12 |
| BBa_K1379018 | RNA | HKUST Lock 1 | 45 |
| BBa_K1379019 | RNA | HKUST Key 1 | 82 |
| BBa_K1379020 | Intermediate | Lock 1-GFP-B0015 | 903 |
| BBa_K1379022 | Intermediate | Lock 3-GFP-B0015 | 905 |
| BBa_K1379024 | Intermediate | Lock 3c-GFP-B0015 | 905 |
| BBa_K1379026 | Intermediate | K175031-GFP-B0015 | 910 |
| BBa_K1379028 | Intermediate | HKUST Lock 1-GFP-B0015 | 908 |
| BBa_K1379046 | Intermediate | RBS(Lock 1)-GFP-B0015 | 873 |
| BBa_K1379047 | Intermediate | RBS(Lock 3c)-GFP-B0015 | 875 |
| BBa_K1379048 | Measurement | J23102-Lock 1-GFP-B0015-pBad/araC-Key 1-B0015 | 2403 |
Parts that we constructed and submitted
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_I0500 | Inducible pBad/araC promoter | Inducible pBad/araC promoter | 1210 |
| BBa_J01010 | Riboregulator Lock 1 | Biobrick version of Isaacs' riboregulator cis repressed lock, crR12. | 40 |
| BBa_J01080 | Lock3 | Biobrick version of Isaacs' riboregulator cis repressed lock, crR12, and modified version of Riboregulator Lock 1. | 40 |
| BBa_J01086 | Key3 | Biobricked version of Isaacs' riboregulator trans activating key, taR12, and modified version of Riboregulator Key 1. | 94 |
| BBa_K175031 | Lock for medium RBS (B0032) from the lock/key library TUD09 | This biobrick generates a mRNA which forms a secondary structure blocking the (medium) RBS. | 47 |
| BBa_K175032 | Key for lock of medium (K175031) RBS (B0032) from the lock/key library TUD09 | This biobrick generates a mRNA which forms a secondary structure that opens LMR (K175031). | 84 |
| BBa_J23080 | B0034 derived RBS to match spacing of J01122 | RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122. | 10 |
Parts that already exist in Parts Registry and are currently under characterization
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_J01008 | Riboregulator Lock 1 | Lock 1 | 94 |
| BBa_J23031 | Lock 3c | lock3c | 42 |
| BBa_J23008 | Key 3c | Encodes a short RNA sequence that "unlocks" the biobrick part J01122 (lock3) which is locked RFP. | 42 |
Parts that we designed but did not submit
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1379021 | Intermediate | Key 1-B0015 | 231 |
| BBa_K1379023 | Intermediate | Key 3-B0015 | 231 |
| BBa_K1379025 | Intermediate | Key 3c-B0015 | 231 |
| BBa_K1379027 | Intermediate | K175032-B0015 | 221 |
| BBa_K1379029 | Intermediate | HKUST Key 1-B0015 | 219 |
| BBa_K1379030 | Measurement | J23102-Lock 1-GFP-B0015 | 946 |
| BBa_K1379031 | Intermediate | pBad/araC-Key 1 | 1312 |
| BBa_K1379032 | Measurement | J23102-Lock 3-GFP-B0015 | 948 |
| BBa_K1379033 | Intermediate | pBad/araC-Key 3 | 1312 |
| BBa_K1379034 | Measurement | J23102-Lock 3c-GFP-B0015 | 948 |
| BBa_K1379035 | Intermediate | pBad/araC-Key 3c | 1312 |
| BBa_K1379036 | Measurement | J23102-K175031-GFP-B0015 | 953 |
| BBa_K1379037 | Intermediate | pBad/araC-K175032 | 1302 |
| BBa_K1379038 | Measurement | J23102-HKUST Lock 1-GFP-B0015 | 951 |
| BBa_K1379039 | Intermediate | pBad/araC-HKUST Key 1 | 1300 |
| BBa_K1379049 | Measurement | J23102-Lock 1-GFP-B0015-pBad/araC-Key 1-B0015 |
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