X-press: Characterization of Team Heidelbergs pSBX-vectors
The iGEM team Heidelberg constructed a set of expression vectors that contain a T7 promoter prior to the BioBrick prefix. This way RBS-CDS parts can be cloned into the vectors by standard BioBrick assembly and then expressed in BL21(DE3) by induction with IPTG.
In collaboration with the iGEM Team Heidelberg the iGEM Team Aachen tested 8 of these vectors for their expression capabilities.
The Team Heidelberg expression vectors
These are the tested constructs:
| Plasmid | Backbone | Insert | Length vector [bp] | Length Insert [bp] | Resistance
|
| pSBX1A3-BBa_13507 | pSBX1A3 (high copy) | BBa_13507 | 3067 | 1226 | 50 mg/ml Ampicilin
|
| pSBX1C3-BBa_13507 | pSBX1C3 (high copy) | BBa_13507 | 2982 | 1226 | 35 mg/ml Chloramphenicol
|
| pSBX1K3-BBa_13507 | pSBX1K3 (high copy) | BBa_13507 | 3116 | 1226 | 50 mg/ml Kanamycin
|
| pSBX1T3-BBa_13507 | pSBX1T3 (high copy) | BBa_13507 | 3322 | 1175 | 10 mg/ml Tetracyclin
|
| pSBX4A5-BBa_13507 | pSBX4A5 (low copy) | BBa_13507 | 4307 | 1192 | 50 mg/ml Ampicilin
|
| pSBX4C5-BBa_13507 | pSBX4C5 (low copy) | BBa_13507 | 4127 | 1186 | 15 mg/ml Chloramphenicol
|
| pSBX4K5-BBa_13507 | pSBX4K5 (low copy) | BBa_13507 | 4331 | 1192 | 50 mg/ml Kanamycin
|
We also used an mRFP selection marker with a constant expression of mRFP as a positive control.
To find out more about the Results of the characterization check out the the collaboration page of Team Heidelberg.
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