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Team:OUC-China/Judging Achievment
From 2014.igem.org
Achievements
In our human practice project we achieved……
1.Last November, we went to Peking University to have an academic communication with 2013 Peking iGEM team.
2.Consulted professor on several problems in Qingdao Institute of Biomass Energy and Bioprocess Technology, Chinese Academy of Sciences. Besides, Dr. Huang made a professional lecture about digital simulation and bioapplication, and provided us advice on modeling.
3.Invited Beijing Normal University iGEM team to our university. We had a discussion on our projects, and we exchanged gifts to each other.
4.Hold the Fourth Science and Technology camp for new sophomores and passed some knowledge of synthetic biology to them. We also had a small jamboree for them.
5.Attended the Central China iGEMers’ Consortium (C C i C) hold on Wuhan, China. We presented our project and communicated with others teams in China.
6.We activated and sent two standard biological parts of gas vesicle genes , GvpA and GvpC to Peking iGEM team. And we have helped Peking to test the function of the two parts to make bacteria float.
7.To evaluate our project’s application in future, we visited the fish farms and consulted the engineers about the ways of curing and precaution in terms of fish diseases. Besides, we also visited Hu Haibin who is the most authoritative expert in aquaculture feeds in China. And we got information about combined coated pellets technology. If we combined coated pellets technology with our projects, we could apply the feed with outer membrane on the ornamental fish industry.
8.In terms of the transportation of biological products, we finished the investigation. And we gave our solutions to the problems.
In terms of the double plasmids system
1.Test of the conjugation ability of BBa_J01003.
2.Construction of the parts: BBa_K1439000, BBa_K1439001, BBa_K1439002, BBa_K1439003.
3.Construction of the mini plasmid (OriTRP4-BBa_J04450-pSB1C3).
4.Proved that the mini plasmid can’t transfer if there is no plasmid RP4.
5.Constructed the double plasmids system with mini plasmid (OriTRP4-BBa_J04450-pSB1C3) and plasmid RP4. And proved that the mini plasmid can transfer between cells with the help of RP4.
6.Provement of conjugation between HB101 and Vibrio harveyi.
In terms of Transfection
1.We successfully proved that our recombinant plasmid (pet32a) can produce the H4::TAT protein as we expected.
2.We successfully extracted and purified fusion protein, proving the protect function of DNA.
3.Via RT-PCR experiments, we successfully proved the protein and plasmid complex could transfect into fish.
