Team:UI-Indonesia/Project/Approach and Overview

Approach

Nowadays, the only way to banish biofilm is by avoiding its excretion on the first place. However, in cases when the biofilm has settled in the digestive tract, avoiding it is no longer possible. Synthetic biology is one of the developing knowledge in our everyday world. Here we proposed the novel strategy to combat biofilm infection by using ‘Genius E.coli (GE)’, one will be able to detect quorum sensing signal and destroy biofilm made by pathogenic bacteria. E. coli which is a normal flora in the intestines can be modified to secrete components such as alpha amylase, nuclease, and substilisin that will be able to destroy biofilm made by pathogenic bacteria and novel synthetic peptide called peptide 1018, originated from human immunity defense regulator which is enable target cellular stress responses prevent and eradicate biofilm formation.

Overview

Our team use Vibrio cholerae as pathogen model. Vibrio cholerae is an agent cause cholera disease. In general, the incidence of this disease reaches 500 cases out of 100,000 people annually^[1]. In children aged less than one year, the incidence is 4 out of 1.000 cases per year^[1]. Cholera is one of the diseases with dangerous clinical manifestations, and can even cause death when left untreated. The incidence of cholera increase when natural disaster occurred. Diarrheal disease happened after drinking water has been contaminated. An epidemic of diarrheal disease after flooding in Bangladesh in 2004 engage more than 17,000 cases; Vibrio cholerae^[1][1].