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Team:Hong Kong HKUST/riboregulator/parts
From 2014.igem.org
Riboregulator Parts
Parts that we designed and submitted
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1379015 | Lock 3C RBS | B0032 derivate for Lock 3C | 12 |
| BBa_K1379018 | HKUST Lock 1 | Cis-Repressor HKUST Lock 1 | 45 |
| BBa_K1379019 | HKUST Key 1 | Trans-Activator for HKUST Key 1 | 82 |
| BBa_K1379020 | Cis-repressed GFP Lock 1 | Cis-repressed GFP Generator Lock 1 | 903 |
| BBa_K1379022 | Cis-repressed GFP Generator Lock 3 | Cis-repressed GFP Generator Lock 3 | 905 |
| BBa_K1379024 | Cis-repressed GFP Lock 3C | Cis-repressed GFP Generator Lock 3C | 905 |
| BBa_K1379026 | Cis-repressed GFP Lock MCR | Cis-repressed GFP Generator MCR | 910 |
| BBa_K1379028 | Cis-repressed GFP HKUST Lock 1 | Cis-repressed GFP Generator HKUST Lock 1 | 908 |
| BBa_K1379046 | Lock 1 RBS GFP generator | GFP generator using a Lock 1 modified RBS | 873 |
| BBa_K1379047 | Lock 3C RBS GFP generator | GFP generator using a Lock 3C modified RBS | 875 |
Parts that we constructed and submitted
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_I0500 | Inducible pBad/araC promoter | Inducible pBad/araC promoter | 1210 |
| BBa_J01010 | Riboregulator Lock 1 | Biobrick version of Isaacs' riboregulator cis repressed lock, crR12. | 40 |
| BBa_J01080 | Lock3 | Biobrick version of Isaacs' riboregulator cis repressed lock, crR12, and modified version of Riboregulator Lock 1. | 40 |
| BBa_J01086 | Key3 | Biobricked version of Isaacs' riboregulator trans activating key, taR12, and modified version of Riboregulator Key 1. | 94 |
| BBa_K175031 | Lock for medium RBS (B0032) from the lock/key library TUD09 | This biobrick generates a mRNA which forms a secondary structure blocking the (medium) RBS. | 47 |
| BBa_K175032 | Key for lock of medium (K175031) RBS (B0032) from the lock/key library TUD09 | This biobrick generates a mRNA which forms a secondary structure that opens LMR (K175031). | 84 |
| BBa_J23080 | B0034 derived RBS to match spacing of J01122 | RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122. | 10 |
| BBa_K1379048 | Cis repressed GFP, with Trans-activator | Repression measurement device 1 with trans activator | 2403 |
Parts that already exist in Parts Registry and are currently under characterization
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_J01008 | Riboregulator Lock 1 | Lock 1 | 94 |
| BBa_J23031 | Lock 3c | lock3c | 42 |
| BBa_J23008 | Key 3c | Encodes a short RNA sequence that "unlocks" the biobrick part J01122 (lock3) which is locked RFP. | 42 |
Parts that we designed but did not submit
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1379021 | Key 1 Double Terminator | Trans-Activator with terminator for lock 1 | 231 |
| BBa_K1379023 | Key 3 double terminator | Trans-Activator with terminator for lock 3 | 231 |
| BBa_K1379025 | Key 3c double terminator | Trans-Activator with terminator for lock 3C | 231 |
| BBa_K1379027 | MTA double terminator | Trans-Activator with terminator for MCR | |
| BBa_K1379029 | HKUST Key 1 double terminator | Trans-Activator with terminator for HKUST Lock 1 | |
| BBa_K1379030 | Cis-Repressing device 1 | Constitutive promoted, Cis-repressed GFP Coding device Lock 1 | |
| BBa_K1379031 | AraC Key 1 | AraC inducible Trans-Activator device, Key 1 | |
| BBa_K1379032 | Cis-Repressing device 3 | Constitutive promoted, Cis-repressed GFP Coding device Lock 3 | |
| BBa_K1379033 | AraC Key 3 | AraC inducible Trans-Activator device, Key 3 | |
| BBa_K1379034 | Cis-Repressing device 3C | Constitutive promoted, Cis-repressed GFP Coding device Lock 3C | |
| BBa_K1379035 | AraC Key 3C | AraC inducible Trans-Activator device for, Key 3C | |
| BBa_K1379036 | Cis-Repressing device MCR | Constitutive promoted, Cis-represed GFP Coding device Lock MCR | |
| BBa_K1379037 | AraC MTA | AraC inducible Trans-Activator device, MTA | |
| BBa_K1379038 | Cis-Repressing device HKUST 1 | Constitutive promoted, Cis-repressed GFP Coding device HKUST Lock 1 | |
| BBa_K1379039 | AraC HKUST Key 1 | AraC inducible Trans-Activator device, HKUST Key 1 | |
| BBa_K1379040 | Repression device 1 | Repression measurement device 1 | |
| BBa_K1379041 | Repression device 3 | Repression measurement device 3 | |
| BBa_K1379042 | Repression device 3C | Repression measurement device 3C | |
| BBa_K1379043 | Repression device Medium | Repression measurement device Medium | |
| BBa_K1379044 | Cis-Repression device HKUST 1 | Repression measurement device HKUST 1 | |
| BBa_K1379049 | Cis repressed GFP lock 1, with Trans-activator key 1 | Cis repressed GFP lock 1, with Trans-activator key 1 |
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