Team:Caltech/Project/Methods and Methods

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Materials and Methods
Overall Project Summary

Project Details

Materials and Methods

The Experiments

Results

Data Analysis

Conclusions

References

PCR

 For each 25 uL reaction mixture:
  • 12.5 uL Phusion Mastermix
  • 2.5 uL primer mix (10 uM of forward and reverse primer)
  • 1 uL DNA template
  • 0.75 uL DMSO (optional)
  • Fill to 25 uL with MilliQ water

Gel electrophoresis
  • blah blah

Colony PCR
  • blah blah
  • blah blah
  • blah blah
  • blah blah

Gibson assembly
  • whreee
  • blah blah
  • blah blah
  • blah blah
  • blah blah

PCR purification
  • Used protocol and kits provided by Qiagen

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