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Team:Berlin cn
From 2014.igem.org
iGEM BERLIN
具有磁性的E型大腸桿菌?
具有磁性的E型大腸桿菌?為能通過磁力遠程操控細菌，需對細菌進行對鐵元素的吸收及合成的優化。從而達到對其進行磁力遠程操控的目的。具有豐富用途，微小而高效的大單元則成為最終結果。
基於這些細胞在生物治療藥物，生物材料及工藝技術領域提供了新方案，它們是極具吸引力的。此外，我們還將通過計算機界面對這些細菌細胞進行實時遠程操控。
Project
In October 2014, a team of Berlin students from a variety of backgrounds are participating for the first time in the international genetically engineered machines competition. For our project, the iGEM Berlin team has decided to make E. coli bacteria susceptible to magnetic fields. By knocking out the iron efflux transporter (FieF) and iron uptake suppressor (FUR), we want to increase the total iron level of the cytosol. By sequestering iron in a ferritin protein, iron crystals are formed and the cell is detoxified. In order to create ferromagnetic of super paramagnetic crystals, we will then use an intensive high through put media optimization to discover the best conditions for the formation of magnetic nanoparticles in E. coli. Furthermore, we will work with other metal binding proteins like metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis in E. coli. Once we have discovered the best way to magnetize E. coli bacteria we will build and characterize a suitable BioBrick that can be used by any research lab or iGEM team in the world in order to remote control the movement of E. coli cells.
Team
The iGEM Team Berlin consists of 17 students from various universities in Berlin. Represented are the Technische Universität Berlin, University of Applied Sciences Berlin, Freie Universität as well as the Humboldt University. The team members come from diverse disciplines. From biology, biotechnology, biochemistry, computer science, mathematics, mechanical engineering to the history of science and technology, everything is represented. Just the interdisciplinary nature of the team allows us to jointly develop solution strategies from different perspectives. Hosted by Prof. Dr. Nediljko Budisa from the TU Berlin, our lab is located at the Müller-Breslau-Straße 10, 10623 Berlin. Come by for a visit (Monday 5 pm).
Safety
Our country uses a four-part „Safety Level“ rating system for laboratories. Level 4 is used for the most dangerous organisms. This is equivalent to the WHO system. The Safety Level of our lab is Level 1 (low risk).
Sponsors
