Team:Tuebingen/Notebook/Protocols/OligoAnnealing

From 2014.igem.org

Revision as of 15:23, 17 October 2014 by SvenB (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Oligo Annealing

Reagents

96 µL	TE-buffer + 56 mM NaCl
2 µL	Primer forward
2 µL	Primer reverse

Procedure

Dilute the primers in buffer.
Heat the solution to 98 °C and decrease temperature slowly to 25 °C.
Use for ligation.

Retrieved from "http://2014.igem.org/Team:Tuebingen/Notebook/Protocols/OligoAnnealing"