Team:Groningen/testenv

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Primers for making BioBricks out of the nisin operon
 
Primer Sequence
NisA forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA
NisA reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA
NisB forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAAAAGTTCATTTAA
NisB reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCATGTATTCTT
NisT forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA
NisT reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT
NisC forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATAAAAAAAATATAAA
NisC reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCCTCTTCCCTC
NisI forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGAAGATATTTAATACT
NisI reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTAATTTCCTACCTTCG
NisP forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAAAAAATACTAGGTTT
NisP reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC
NisRK forward GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGTATAAAATTTTAATAGT
NisRK reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA
NisFEG forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA
NisFEG reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT
PNisI+RBS forward GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCACTGGATAATGACTATT
PNisI+RBS reverse TACTAGTAGCGGCCGCTGCAGGAAGAAACTTCCTCTTCCCTCCTTTCAA
 
 
 
 
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Primers for making BioBricks out of the nisin operon
 
Primer Sequence
NisA forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA
NisA reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA
NisB forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAAAAGTTCATTTAA
NisB reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCATGTATTCTT
NisT forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA
NisT reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT
NisC forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATAAAAAAAATATAAA
NisC reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCCTCTTCCCTC
NisI forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGAAGATATTTAATACT
NisI reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTAATTTCCTACCTTCG
NisP forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAAAAAATACTAGGTTT
NisP reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC
NisRK forward GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGTATAAAATTTTAATAGT
NisRK reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA
NisFEG forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA
NisFEG reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT
PNisI+RBS forward GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCACTGGATAATGACTATT
PNisI+RBS reverse TACTAGTAGCGGCCGCTGCAGGAAGAAACTTCCTCTTCCCTCCTTTCAA
 
 
 
 
 
 
© 2014 iGem Groningen, Design: Randy Wind; Art: Andries de Vries, Freek de Wijs and Bente Lauxen; Photography: Bente Lauxen; Coding: Randy Wind and Anco Gietema.
 
 
 
 
 

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