"
Team:Groningen/testenv
From 2014.igem.org
Test Environment
>
Hello
<html>
<!--Content module-->
<div class="module contentmodule gridcell">
<div class="content">
<div class="wrapper">
<!-- TITLE SNIPPET MANDATORY START-->
<div class="title">
Title here, no images!!!
</div>
<div class="hspacer"> </div>
<!-- TITLE SNIPPET MANDATORY END-->
<!-- TABLE SNIPPET OPTIONAL START-->
<table class="table">
<tr>
<th>EMPTY</th><th>EMPTY</th>
</tr>
<tr>
<td>EMPTY</td><td>EMPTY</td>
</tr>
<tr>
<td>EMPTY</td><td>EMPTY</td>
</tr>
<tr>
<td>EMPTY</td><td>EMPTY</td>
</tr>
</table>
<div class="hspacer"> </div>
<!-- TABLE SNIPPET OPTIONAL END-->
<!-- SUBTITLE SNIPPET OPTIONAL START-->
<div class="subtitle" >
Used strains S. aureus: (All strains were provided by Auke van Heel)
</div>
<div class="hspacer"> </div>
<!-- SUBTITLE SNIPPET OPTIONAL END-->
<!-- PARAGRAPH SNIPPET MANDATORY START-->
<div class="text">
PARAHRAPH HERE (TEXT ONLY, NO IMAGES!!!)
</div>
<div class="hspacer"> </div>
<!-- PARAGRAPH SNIPPET MANDATORY END-->
<!-- FIGUREMOVIE SNIPPET OPTIONAL START -->
</html>{{:Team:Groningen/Template/MODULE/newfigurevideo|Figure-movie 1
|1/11/Staphilococcus_combat_system.art.png
|1/1e/LactoAid_Explaination_def_met_sponsors_v2.mp4|
Vivamus a augue diam. Nam hendrerit nisi ut tellus hendrerit, ac volutpat massa tristique.
}}<html>
<!-- FIGUREMOVIE SNIPPET OPTIONAL END-->
<!-- FIGURE SNIPPET OPTIONAL START -->
</html>{{:Team:Groningen/Template/MODULE/newfigure|Figure 1|1/11/Staphilococcus_combat_system.art.png|
Vivamus a augue diam. Nam hendrerit nisi ut tellus hendrerit, ac volutpat massa tristique.
}}<html>
<!-- FIGURE SNIPPET OPTIONAL END-->
<!-- LISTING SNIPPET OPTIONAL START-->
<div class="listing">
<div class="item">I'AM A BULLET COPY ME AND EXPLAIN ME.</div>
<div class="hspacer"> </div>
</div>
<div class="hspacer"> </div>
<!-- LISTING SNIPPET OPTIONAL END-->
<div class="hspacer"> </div>
</div>
<div class="hspacer"> </div>
</div>
<div class="hspacer"> </div>
</div>
</html>
Primers for making BioBricks out of the nisin operon
| Primer | Sequence |
|---|---|
| NisA forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA |
| NisA reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA |
| NisB forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAAAAGTTCATTTAA |
| NisB reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCATGTATTCTT |
| NisT forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA |
| NisT reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT |
| NisC forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATAAAAAAAATATAAA |
| NisC reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCCTCTTCCCTC |
| NisI forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGAAGATATTTAATACT |
| NisI reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTAATTTCCTACCTTCG |
| NisP forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAAAAAATACTAGGTTT |
| NisP reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC |
| NisRK forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGTATAAAATTTTAATAGT |
| NisRK reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA |
| NisFEG forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA |
| NisFEG reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT |
| PNisI+RBS forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCACTGGATAATGACTATT |
| PNisI+RBS reverse | TACTAGTAGCGGCCGCTGCAGGAAGAAACTTCCTCTTCCCTCCTTTCAA |
Primers for making BioBricks out of the nisin operon
| Primer | Sequence |
|---|---|
| NisA forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA |
| NisA reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA |
| NisB forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAAAAGTTCATTTAA |
| NisB reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCATGTATTCTT |
| NisT forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA |
| NisT reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT |
| NisC forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATAAAAAAAATATAAA |
| NisC reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCCTCTTCCCTC |
| NisI forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGAAGATATTTAATACT |
| NisI reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTAATTTCCTACCTTCG |
| NisP forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAAAAAATACTAGGTTT |
| NisP reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC |
| NisRK forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGTATAAAATTTTAATAGT |
| NisRK reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA |
| NisFEG forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA |
| NisFEG reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT |
| PNisI+RBS forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCACTGGATAATGACTATT |
| PNisI+RBS reverse | TACTAGTAGCGGCCGCTGCAGGAAGAAACTTCCTCTTCCCTCCTTTCAA |
