Team:UESTC-China/Notebook

Feb 14
iGEM 2014 registration opens.
Feb 20
Site: West 304 of main building in UESTC
Team of iGEM 2014 in UESTC is founded.
Feb 21
Site: West 304 of main building in UESTC
We name our team Green Life;
Discuss the direction we will focus on for the iGEM 2014.
Feb 24
Site: West 304 of main building in UESTC
We meet the supervisors Yong Zhang, Xuelian Zheng, Lixia Tang, discussing the direction and acquiring their opinions of our project.

March 3
Site: West 304 of main building in UESTC
We firstly choose plant as our receptor, each member of our team give advices for the details we plan to do, such as which plant to use, tobacco, arabidopsis or any other plant?
March 10
Site: West 304 of main building in UESTC
We talk with our supervisors and decide to use tobacco as our receptor for its simpleness as model organism.
March 17
Site: West 304 of main building in UESTC
We discuss the project and firstly decide to create a plant which can absorb formaldehyde largely by synthetic biology. And we choose Yasong Cui as our team leader.
March 24
Site: West 304 of main building in UESTC
Team leader assign task for us;
The first phase for our task begins, searching references and designing our logo.
March 31
We register our team with the name of Green Life;
iGEM 2014 registration closes;
Team registration fee due.

April 9
Site: West 304 of main building in UESTC
Group meeting for the references and information we have researched last few days. Team leader Yasong Cui and Jingyao Li report the key enzymes through the formaldehyde metabolic pathways. HPS, PHI are critical enzymes of RuMP pathway in formaldehyde assimilation. The references show that the transformation method is chloroplast transformation. The crucial enzyme in amplifying stomas of foliage is AHA2.
April 13
Site: West 304 of main building in UESTC
Group meeting for the reference reading.
Mingyuan Wang reports one gene (AdCP) of pollen sterility;
Jiao He reports FDH which can amplify metabolic pathway in plant;
Yasong Cui reports gene AHA2 which can amplify stoma of foliage.
April 16
Site: West 304 of main building in UESTC
Group meeting for the wiki constructing;
The responsible person of wiki, Zhiqiang Yan assigns tasks for the rest.
April 20
Site: West 304 of main building in UESTC
Jie Li reports FALDH which can amplify metabolic pathway in plant;
Rui Liu reports some information about HPS, PHI;
Yasong Cui reports some information about AHA2.
April 23
Site: West 304 of main building in UESTC
Vector construction and confirmation of the gene (HPS, PHI, FDH, FALDH, AHA2, AdCP) being used in our project.
April 23
Site: online
We exchange the team name Green Life into UESTC-China.
April 25
Start to design a questionnaire about iGEM and our project. And plan to do a small range research.
April 30
Site: The seventh middle school in Chengdu.
We introduce the concept and racing type of iGEM to the students in the seventh middle school who were the best students in Sichuan province.
Site: West 304 of main building in UESTC
We talk about the PrbcS-3C promoter sequence and the gene sequence of transit peptide of chloroplast.

DNA Distribution Kit sent to teams
May 01
iGEM 2014 Late registration closes
May 3
Site: West 304 of main building in UESTC
Decision on the backbone vector.
The backbone named pXZY006 is proffered by one of our advisor Zhengyang Xie. And we designate it piGEM001.
Weixin Liu finds some chloroplast and cytoplasm transit peptide from Uniprot database.
May 7
Site: West 304 of main building in UESTC
Take a group meeting and design the vectors we plan to use.
May 14
Site: West 304 of main building in UESTC
Group meeting.
We discuss some details in the designing of vector constructing;
At the end of the meeting, we started our experiments.
May 19
Site: West 121 of main building in UESTC
We extract pXZY006 plasmid from bacterium culture using TIANGEN kit.
Using SpeⅠand HpaⅠto do the double digestion of pXZY006 and F01 (contains HPS ) and do the ligation using T4 DNA ligase.
May 22
Site: West 121 of main building in UESTC
Do the transformation in competent recipient E.coli cell.
May 23
Site: West 121 of main building in UESTC
Repeat the experiments we do in May 19 and May 22 for the outcome is not that satisfactory.
Also we do not use the T4 ligase to do the ligation but by Gibson assembly.
May 28
Site: West 121 of main building in UESTC
Do the colony PCR to testify if the F01 has ligated to pXZY006.
And it comes out with positive clones, so the vector piGEM001 has been successfully constructed.
May 29
Site: West 304 of main building in UESTC
Group meeting;
Decide to build the second vector piGEM002.
Ligate F05, F02, and F03 to piGEM001 to construct piGEM002;
Ligate F06, F07 to piGEM002 to construct piGEM011.
May 30
Site: West 121 of main building in UESTC
Ligate F06 and F07 to iGEM001.
May 31
Site: West 121 of main building in UESTC
Do the transformation using heat shock.
The outcome is satisfactory that there is no clone in control group and thirteen clones in experimental group.

June 5
Site: West 121 of main building in UESTC
Do the colony PCR to testify if the F06 and F07 have ligated to piGEM001.
This ligation fail due to the outcome of colony PCR.
June 6
Site: West 121 of main building in UESTC
Repeat the experiments we did on May 31.
June 7
Site: West 121 of main building in UESTC
Do the transformation and colony PCR.
The outcome of colony PCR still demonstrate we fail again.
June 8-13
Site: West 121 of main building in UESTC
We do the ligation and transformation repeatedly, asking help from our advisors, finally we got the right outcome of piGEM011 (not the originally piGEM011 we planned to construct, used to be F06+F07+piGEM002 and now is F06+F07+piGEM001).
June 14
Site: West 121 of main building in UESTC
We ligate F02, F03, and F05 but fair.
June 15
Site: West 121 of main building in UESTC
We ligate F02, F03, and F05 but fair.
June 16
Site: The area before Sun Shine dining hall in UESTC
We make a knowledge quiz which contains a few questions concerning iGEM or synthetic biology to expand people's horizon.
June 19
Open Office Hours -- ask safety questions, meet other iGEMers!
June 23
About our Lab form due
June 17-30
Site: West 121 of main building in UESTC
We do the multiple ligation of F02, F03, and F05 repeatedly for changing the parameters in experiments but fair.

July 1-16
Site: West 121 of main building in UESTC
We do the multiple ligation of F02, F03, and F05 repeatedly for changing the parameters in experiments but fair. And finally we abandon F03 and F05, ‘cause no matter how we improve the criteria of our experiments we still get the fail result. But we still add F02 into piGEM001 to construct the backbone.
July 13
Site: West 121 of main building in UESTC
Start to construct DNA samples in pSB1C3.
July 16
Site: West 121 of main building in UESTC
We ligate F02 to piGEM001 as the new vector piGEM002.
July 21
Preliminary safety forms due.
July 23
Site: West 121 of main building in UESTC
Group meeting.
We decide to construct the rest vectors piGEM003, piGEM004,piGEM005, piGEM006, piGEM007, piGEM008, piGEM009, piGEM010.
July 25
Track selection due;
Site: West 121 and west 111 of main building in UESTC
We communicate with the students from SCU. We exchange our project to each other and make a long talk about what we have done during the last month.
July 26
Site: West 121 and west 111 of main building in UESTC
From July 25th,we start to do the transformation part. That really requires large volume of work. Four members of our team do the most of the work,and other members also help a lot.
July 27
Site: Before the main building of UESTC
The members of our team gather and take a photo.
July 28
Site: West 121 of main building in UESTC
Start to construct the vector piGEM003, piGEM004, piGEM005, piGEM006, piGEM008, piGEM010.
July 29
Site: West 121 of main building in UESTC
Go on revising the questionnaire about iGEM. And plan to start a second research.
July 28-31
Site: West 121 of main building in UESTC
Go on constructing the vector piGEM003, piGEM004, piGEM005, piGEM006, piGEM008, piGEM010.
Start to construct piGEM007.

August 2
Site: West 121 of main building in UESTC
Start to construct the vector piGEM009.
Do the colony PCR in the eight vectors to detect if the colony is positive or not.
August 3
Site: West 121 of main building in UESTC
Amplify 6*A-MASS, GSG-P2A, GSG-T2A, GSG-F2A by PCR.
August 5
Site: West 121 of main building in UESTC
Amplify AtAHA2 by PCR.
August 8
Site: The playground of UESTC and the opening area in front of supermarket
We go to the playground and do the research for our questionnaire.
August 11
Site: West 121 of main building in UESTC
All the vector (piGEM003-010) we planned to build has finished.
August 15
Team project descriptions due.
August 18
Site: West 206 of main building in UESTC
During the summer vacation, besides communicating with the students in SCU, there was another activity which worth a notice, our principal came to our laboratory and gave us a speech, which totally stimulated us to work harder and excited. The principal told us do not be scared to have the new idea, 'cause the study we received today is to make us to speak out a new idea no matter it is wizard or not.
August 20
Site: West 121 of main building in UESTC
For about two months work, our wiki has its fundamental appearance.
August 27
Site: West 304of main building in UESTC
Group meeting for the progress of our project.
August 30
Site: Qingshui river campus of UESTC
To improve the outcome of our iGEM, we start to do a third research for our questionnaire today. 'cause the people from various places gather in our school for accompanying their children to go to school.

September 1 to now
Site: West 121 of main building in UESTC
On September 1st, when the eighth leaves of the first batch of tobacco seedlings appear, we start the formal experiments of formaldehyde detection. Before that, some pre-tests are made based on some corresponding references. Finding the right amount of formaldehyde is the hardest part. By means of a series of experiments,we determine the final protocol which includes the qualitative detection and quantitative detection. From the experimental results, we can draw the conclusion that our super plants have remarkable formaldehyde tolerance and can dramatically reduce the concentration of formaldehyde in the air.
September 1
Final safety forms due.
September 5
Giant Jamboree attendance fee due at 11:59PM EDT;
Team rosters due.
September 6
Site: West 213 of main building in UESTC
Extract the DNA and RNA from transgenosis plant, testifying.
Team rosters due.
September 7-15
Site: West 121 of main building in UESTC
Sum up the information of the questionnaire, and draw up the statistics.
September 20-30
Site: West 121 of main building in UESTC
Analyze the statistics we get from the questionnaire. And start to consider how to present the conclusions on the wiki.
September 24
Site: West 121 of main building in UESTC
The DNA samples in pSB1C3 have been completely finished.
September 25
Site: West 121 of main building in UESTC
In the beginning of this new semester, our school held a science and culture activity. We caught this opportunity to advertise iGEM and our ultimate products, the plants which can absorb formaldehyde. The leaders and many teachers in our school also participated this activity. We displayed the plants which already owned the several genes which worked in the absorption of formaldehyde, to the people who visited our project. And the people visited our plant all said they want to owe one if there is already market available.

October 1
Site: West 121 and west 111 of main building in UESTC
Examine the formaldehyde tolerance of the transgenosis plant and wild type plant.
October 5
Site: West 121 and west 111 of main building in UESTC
Examine the formaldehyde tolerance of the transgenosis plant and wild type plant.
October 3-8
Site: West 121 and west 111 of main building in UESTC
Improve the outcomes of Questionnaire researching.
October 3-8
Site: West 121 and west 111 of main building in UESTC
Keep on constructing our wiki and doing the examinations of our super plant’s tolerance of formaldehyde.
October 9 to now
Site: West 121 and west 111 of main building in UESTC
Keep on constructing our wiki, doing the examinations of our super plant’s tolerance of formaldehyde and extract DNA and RNA from transgenosis tobacco.
October 10
Site: West 121 and west 111 of main building in UESTC
We mimic the iGEM jamboree spot and to present our project to the teachers.
Up to October 17
We keep on constructing our wiki, examining the writing error and lack of standardization and replenishing the picture and results of our project.
Up to now
We keep on replenish our experiments, and examining the formaldehyde tolerance of transgenosis plant. And continue to picture the differences of formaldehyde tolerance in transgenosis and wild type plant.