Team:TU Darmstadt/Materials/Primers

From 2014.igem.org

Revision as of 11:56, 17 October 2014 by S Barthel (Talk | contribs)

Home


Pathway

RBS_CHI_pre
GTAGAGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTCCTCCAGTGTCCGTTAC
 
RBS_CHS_pre
GTAGAGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCGTCCTCCGTTGACATGAAGGCG
 
CHI_psb1c3_Pre
TAATAAGAATTCGCGGCCGCTTCTAGATGGCTCCTCCAGTGTCCGTTAC
 
CHI_psb1c3_Suf2
TTATTACTGCAGCGGCCGCTACTAGTACTAGACTCCAATCACTGGAATAGTAGATTTCTCGG
 
CHS_psb1c3_Pre2
TAATAAGAATTCGCGGCCGCTTCTAGATGGCGTCCTCCGTTGACATGAAGGCGATCAGAG
 
CHS_pscb1c3_Suf2
TTATTACTGCAGCGGCCGCTACTAGTATTAGACGGGAACCGTCACGGTGGTTGGGAC
 
QCIII_CHI_F
GAAGTTTCTGGATGTTTTCCGGAGTGAAACTTTTCCACCTGG
 
QCIII_CHI_R
CCAGGTGGAAAAGTTTCACTCCGGAAAACATCCAGAAACTTC
 
QCI_CHI_F
CGGCGATAGGTGTGTATCTGGAAGAGAGTGCTATTCCTTTTCTGGCCG
 
QCI_CHI_R
CGGCCAGAAAAGGAATAGCACTCTCTTCCAGATACACACCTATCGCCG
 
DFR_mut_EcoRI_577_F
TACGCAGCAGAAAACAATCTCGAATTTATCAGTATCATTCCACCGC
 
DFR_mut_EcoRI_577_R
ATGCGTCGTCTTTTGTTAGAGCTTAAATAGTCATAGTAAGGTGGCG
 
DFR_mut2_EcoRI_622_F
CGCCACCTTACTATGACTATTTAAGCTCTAACAAAAGACGACGCAT
 
DFR_mut2_EcoRI_622_R
GCGGTGGAATGATACTGATAAATTCGAGATTGTTTTCTGCTGCGTA
 
DFR_Prefix
GCGCGAATTCGCGGCCGCTTCTAGATGGTTTCTAGTACAATTAACGAGAC
 
DFR_Suffix
GCGCTACTAGTAGCGGCCGCTGCAGTTAAGCAGATCCATTTTCATGATGT
 
DFR_RBS_Prefix
GTAGGAATTCGCGGCCGCTTCTAGAAAGAGGAGAAATACTAGATGGTTTCTAGTACAATTAACGAGACAC
 
F3H_Prefix
GATGGAATTCGCGGCCGCTTCTAGATGGCTCCTTCAACTCTCAC
 
F3H_RBS_Prefix
GATGGAATTCGCGGCCGCTTCTAGAAAGAGGAGAAATACTAGATGGCTCCTTCAACTCTCAC
 
F3H_Suffix
GCGCCTGCAGCGGCCGCTACTAGTATCAAGCGAAAATTTCGTCTGCG
 
F3H_mut_EcoRI_fwd_820_F
GCCGACCACCAAGCAGTTGTGAACTCCAATTCTAGCAGAATGTCAAT
 
F3H_mut_EcoRI_fwd_820_R
ATTGACATTCTGCTAGAATTGGAGTTCACAACTGCTTGGTGGTCGGC
 
F3H_mut_PstI_fwd_1047_F
GAAGTGGAGAAGGCCAAGCTGCAAATGACACCCAAGAGC
 
F3H_mut_PstI_fwd_1047_R
GCTCTTGGGTGTCATTTGCAGCTTGGCCTTCTCCACTTC
 
eANS_reverse
ATAATTCTGCAGCGGCCGCTACTAGTAATGATTTTTTCTTTTGGCGGTTCACAAAACAC

Nach oben

Scaffold

Scaff pET21a+ up
ATATCATATGGGATCTACCAAAGCAGATATTGGCAC
 
Scaff pET21a+ lo
ATATCTCGAGAGATCCGCTGCCGCTCCCACTA
 
Scaff-His up 2 right
ATGCTAGGAATTCGCGGCCGCTTCTAGATGGGATCTACCAAAGCAGATATTGGCACC
 
Scaff-His lo
TATACTGCAGCGGCCGCTACTAGTATTAGTGGTGGTGGTGGTGGTGCTCG
 
SHC up
ATATGAATTCGCGGCCGCTTCTAGATGGGATCTACCAAAGCAGATATTGGC
 
SHC lo
ATATCTGCAGCGGCCGCTACTAGTATCAGCAGTGGTGGTGGTGGTG
 
SH3-F
ATGCTAGGAATTCGCGGCCGCTTCTAGAAGATCTGCCGAATATGTTCGTGCACTGTTTGA
 
SH3-R
TCATCACTGCAGCGGCCGCTACTAGTAGGATCCTGAGCCACTACCACTACC
 
PDZ-F
ATGCTAGGAATTCGCGGCCGCTTCTAGAAGATCTCTGCAACGTCGTCGTGTTACCGTTCG
 
PDZ-R
TCATCACTGCAGCGGCCGCTACTAGTAGGATCCGCTGCCGCTCCCACTACCG
 
GBD-Met-F
ATGCTAGGAATTCGCGGCCGCTTCTAGATGAGATCTACCAAAGCAGATATTGGCACC
 
GBD-R
TCATCACTGCAGCGGCCGCTACTAGTAGGATCCGCTACCACTGCCGCTACCTGAACC
 
Prefix
GTTTCTTCGAATTCGCGGCCGCTTCTAG
 
Suffix
GTTTCTTCCTGCAGCGGCCGCTACTAGTA

Nach oben

Safety

hokD-fw
GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAGCAGCAAAAGGCGATGTTAATCG
 
hokD-rev
GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTCCTCAGGTTCGTAAGCTGTGAAG

Nach oben