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Team:SF Bay Area DIYbio/Notebook
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WELCOME TO iGEM 2014!Your team has been approved and you are ready to start the iGEM season!
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Notebook
General Protocols
- Electra Cloning Protocol
- DNA Handling Protocol
- Media:Zymo E. coli competent cells transformation protocol.pdf
- Media:D4025i.pdf (Zyppy plasmid midiprep protocol)
- Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf
- Media:Sequencing_Analysis_using_Serial_Cloner_2_16Oct2014.pdf
General Documentation Information
June 2014
August 2014
September 2014
- Cloning 7 casein gBlocks, 12 Sep 2014
- Troubleshooting failed transformation of P.FAKS.humBeta.S on 12 Sept. 2014
- Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
- Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
October 2014
- Initial studies of yeast strains' growth conditions and troubleshooting Oct.2
- Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2
- Cloning human alpha casein S1, 04Oct2014
- Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 08Oct2014
- Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014
- Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 10Oct2014
- Cloning bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 13Oct2014
- Submitting FAM20C (Kex + & Kex -) for sequencing with new internal primers 14Oct2014
- Submitting FAM20C Kex + clone 9A and bovineKappa and bovineBeta: pSB1C3 Biobrick for sequencing 15Oct2014
