ATTRIBUTIONS

Team

The project's experiments were all conducted by the team students. To better tackle each part of the project, we divided the team in small groups which changed depending of the needs of each experiment and the stage of each sub-division.

• Ted Baldwin participated in the characterization of the CpxR and split IFP stress-sensitive response.
• Romane Breysse participated in the characterization of the osmo response with the HOG pathway in S.cerevisiae.
• Jin Chang participated in the characterization of the osmo response with the HOG pathway in S.cerevisiae.
• Axel De Tonnac participated in the design, fabrication and tests of all the different microfluidic chips. He also studied the safety of microfluidic chips.
• Bastien Duckert participated in the characterization of the osmo response with the HOG pathway in S.cerevisiae.
• Arthur Giroux participated in the signal detection and processing with the Raspberry Pi. He also designed the wiki.
• Nikolaus Huwiler participated in the CpxR dimerization characterization. He also improved an existing biobrick (RFP under CpxR promoter).
• Sakura Nussbaum participated in the characterization of the full and split luciferases. She also organized the outreach event at EPFL.
• Lucie Petetin participated in the characterization of the arabinose promoter and of the full and split luciferases. She also participated in the osmo response characterization.
• Cécile Piot participated in the characterization of the CpxR and split IFP stress-sensitive response.
• Ione Pla participated in the characterization of the arabinose promoter. She also took care of submitting the biobricks to the registry and of many administrative tasks.
• Grégoire Repond participated in the characterization of the luciferase. He also participated in the design and tests of the microfluidic chips and studied the safety of the chips.
• Thomas Simonet participated in the characterization of the luciferase. He also participated in the design, fabrication and tests of the microfluidic chips and studied the safety of the chips.

The TAs and Professors gave us regular feedback at the weekly meetings throughout the project.

External Help

Francesco Piraino, Henrike Niederholtmeyer and Francesca Volpetti helped us with autoCAD designing, microfluidic chips fabrication and general help during our experiments with microfluidic chips.

John McKinney and Jan van der Meer discussed the biosafety issues in microfluidics with us, which helped us in our Bio Safety part.

Viesturs Simanis and Andrea Krapp helped us a lot to begin the yeast part of the project. They clarified many points regarding yeast transformations and gene regulation in S. cerevisiae. They confirmed the feasibility of our project and validated the constructs we had designed.