Biosensor
We developed and optimized a novel method of reading out biosensors on a two dimensional level. The sensor cells are therefore immobilized in an agar chip. It was experimentally validated that this method works for different kinds of reporter cells, induction substances and readout methods including our newly designed system WatsOn.
To improve the response of our reporter cells to induction we designed and engineered a novel reporter system using a TEV protease to activate a previously synthesized reporter protein by cleaving off a quencher. A faster response in comparison to regular expression of the reporter protein was shown by modeling as well as in our experiments. All parts needed to build this kind of reporter system were submitted to the partsregistry.
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