Team:NRP-UEA-Norwich/Notebook Protocols
From 2014.igem.org
Lab Protocols
GoldenGate Digestion-Ligation reaction (Level 1)
PROTOCOL HERE
GoldenGate Digestion-Ligation reaction (Level 2)
PROTOCOL HERE
Making LB Agar
PROTOCOL HERE
Making LB broth
PROTOCOL HERE
E. coli Calcium Chloride heat shock Transformation
PROTOCOL HERE
E. coli Electroporation Transformation
PROTOCOL HERE
Making elctrocompetent Agrobacterium tumefaciens
PROTOCOL HERE
Agrobacterium tumefaciens Electroporation Transformation
PROTOCOL HERE
Blue- White Selection
PROTOCOL HERE
Colony PCR
PROTOCOL HERE
DNA mini-prep
PROTOCOL HERE
Preparation for sequencing
PROTOCOL HERE
Agrobacterium tumefaciens Infiltration
PROTOCOL HERE
Infiltration Analysis
PROTOCOL HERE
Making GoldenGate compatible PSB1C3 'Flipper'
PROTOCOL HERE
Antibiotic Selection
PROTOCOL HERE