Team:Hong Kong HKUST/pneumosensor

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Pneumosensor Project Abstract

Figure 1. Pneumosensor Overview Diagram

Quorem-sensing peptide CSP produced by S. pneumoniae is detected by the Detection Module; the response regulator ComE links the Detection Module to the σx Promoters Module which ultimately drives the expression of GFP.

Streptococcus pneumoniae is responsible for approximately 1.6 million deaths every year mostly in developing countries where vaccination is unaffordable. Current methods used for diagnosis of pneumococcal diseases include X-ray of chest, PCR assay using blood and gram stain using sputum. They require a sophisticated equipment, help of a professional doctor or a laboratory setting, all of which are limited to people in third world countries. To provide an affordable solution to combat pneumococcal diseases, we are engineering an E. coli "Pneumosensor", that will work inside as an in vitro diagnostic device. Pneumosensor doesn't require laboratory processing, medical equipment or a doctor for obtaining the diagnosis result, making it more accessible for the people. Through this cheaper and easier diagnosis method, people can prepare themselves better against the transmission and adversity of S. pneumoniae.

Pneumosensor has a rewired comCDE signal transduction pathway native to S. pneumoniae that detects autoinducer molecules released specifically by S. pneumoniae. Upon detection of Streptococcus pneumonia, pneumosensor will give off a fluorescent signal. Apart from the rewired comCDE signal transduction pathway, pneumosensor is also equipped with a new promoter, com-box promoter, for the specific and tight regulation of target gene expression in E. coli.

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