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Team:Bielefeld-CeBiTec/Project/CO2-fixation/CarbonDioxide
From 2014.igem.org
CO2 Fixation
Short summary
In the second module we aim to use a carboxysome which is found in cyanobacteria or purple sulfurbacteria. With this compartment we want to create a Calvin cycle in E. coli. In addition we would like to compare the efficiency of the carboxysome with a free RuBisCO (Ribulose-1,5-bisphosphate-carboxylase-oxygenase), the 3-hydroxypropionate cycle or other types of carboxysomes. The product of the fixation will be pyruvate which can be used for production of different metabolites. As a proof of concept we focus on the production of isobutanol.
Here you will find the results of the CO2 fixation.
Carbon dioxide
Increasing amounts of carbon dioxide has become a major problem in this century. Because of the industrialization typical handmade ware is built by machines which produces carbon dioxide. By changing nearly every production site to industrial production the amount of emission has increased a lot. An additional factor is industrial livestock farming by which methane and carbon dioxide is produced.
The typical balance between consumption and production of carbon dioxide is harmed. The number of forests decreases and the amount of emission increases year by year. Therefore many specialists work on a method to fight the excess of carbon dioxide in the atmosphere.
With our project we also want to engage this problem by using carbon dioxide as a carbon source for E. coli. The next step is the production of a desired product in this bacterium. It is our intention not just to bind carbon dioxide, but to integrate it in usefull products.
