This page outlines our standardized protocols we used in the iGEM project 2014. If we changed one of the protocols for a specific reason, we mentioned it in the laboratory notebook.

In vitro methods

For DNA

- Agarose gel electrophoresis

- Dephosphorylation

- DNA ligation

- DNA quantification with NanoDrop

- DNA sequencing

- PCR purification

- PCR with Pfu polymerase

- Plasmid preparation

- Restriction digest

For Proteins

- SDS-PAGE

In vivo methods

- Bacterial cell culture

- Cell counting/plating

- Chemically competent cells

- Colony PCR with Taq polymerase

- Glycerine stocks

- Heat shock transformation

- Protein expression