Team:UCL/Science/Sequences

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Goodbye Azodye UCL iGEM 2014

Summary

For ease of reference, the sequences for (1) our BioBrick Gene Designs, (2) Plasmid / Vector Backbones, and (3) BioBricks that we have used from that iGEM Registry Distribution, have been provided below. Clicking on the Gene/Plasmid/BioBrick headers will take you to iGEM Parts Registry pages describing the part(s) in more detail.

BioBrick Designs for Our Genes

Registry ID	Gene ID	Name / Function	Source	Size	Status
BBa_K1336000	AzoR	FMN-dependent NADH-azoreductase 1	Pseudomonas putida	612 bp	[In Progress]: primers designed
BBa_K1336001	1B6	AzoR heat-stable mutant	Pseudomonas putida	612 bp	[In Progress]: to remove 2 illegal PstI sites
BBa_K1336002	CotA	Spore Coat Protein Laccase	Bacillus subtilis	1542 bp	[In Progress]: primers designed
BBa_K1336003	BsDyP	Dye Decolourising Peroxidase BSU38260	Bacillus subtilis	1251 bp	[New BioBrick Part]: submitted
BBa_K1336004	PpDyP	Dye Decolourising Peroxidase PP_3248	Pseudomonas putida	864 bp	[In Progress]: primers designed
BBa_K500000	LiP	Lignin Peroxidase	Phanerochaete chrysosporium	1116 bp	[In Progress]: to subclone into pSB1C3/pSB3C5; toxicity issues
BBa_K729006	CueO	Laccase	Escherichia coli	1612 bp	[In Progress]: ascertaining identity
BBa_K1336005	ispB RNAi	RNAi of Octaprenyl Diphosphate Synthase fragment	Escherichia coli, K12 strain	562 bp	[New BioBrick Part]: submitted
BBa_K1336006	LacIEC+ispB	IPTG inducible ispB RNAi	Escherichia coli, K12 strain	2208 bp	[New BioBrick Device]: submitted
BBa_K1336007	LacIEC+BsDyP	IPTG inducible BsDyP	Bacillus subtilis	2208 bp	[New BioBrick Device]: submitted

[New] BBa_K1336000 / AzoR Part-only sequence (612bp)

FMN-dependent NADH-azoreductase 1 (from Pseudomonas putida) [1].
Gene provided by the Microbial & Enzyme Technology Lab led by Dr Lígia O. Martins at the Universidade Nova de Lisboa.
In expression vector: pET-21a (+) (ampicillin resistant (ampR)) [2] [3] , initially cloned between NdeI and BamHI restriction sites.
Sequence obtained from NCBI.

ATG AAA CTG TTG CAC ATC GAT TCG AGC ATC CTG GGC GAC AAT TCC GCC TCG CGT CAG CTG AGC CGT GAA GTG GTC GAA GCC TGG AAG GCT GCA GAC CCA 
AGC GTT GAA GTG GTT TAT CGC GAC CTG GCT GCC GAT GCC ATC GCC CAC TTC TCC GCT GCC ACC CTG GTC GCT GCA GGC ACC CCT GAA GAC GTA CGC GAC 
GCG GCC CAG GCT TTC GAA GCC AAG CTG AGC GCC GAG ACG CTC GAA GAG TTC CTG GCT GCC GAT GCC GTG GTG ATT GGT GCG CCC ATG TAC AAC TTC ACC 
GTG CCG ACC CAG CTC AAA GCC TGG ATT GAC CGC GTA GCC GTC GCC GGC AAG ACC TTC CGC TAC ACC GAA GCC GGC CCG CAA GGC CTG TGC GGC AAC AAG 
AAA GTG GTG CTG GTT TCC ACT GCG GGT GGC CTG CAC GCT GGC CAG CCG ACT GGC GCC GGG CAT GAA GAC TTC CTG AAA GTG TTC CTG GGC TTC ATC GGG 
ATT ACT GAC CTG GAA ATC GTC CGG GCC CAC GGC CTG GCC TAT GGC CCG GAG CAG CGC AGC CAG GCG ATC GAT GCT GCA CAG GCG CAG ATT GCC AGC GAG 
CTG TTT GCG GCT GCC TGA

[New] BBa_K1336001 / AzoR 1B6 (heat-stable AzoR mutant) Part-only sequence (612bp)

Sequence given from Lisbon group directly.

ATG AAA CTG TTG CAC ATC GAT TCG AGC ATC CTG GGC GAC AAT TCC GCC TCG CGC CAG CTG AGC CGT GAA GTG GTC GAA GCC TGG AAG GCT GCA GAC CCA 
AGC GTG GAA GTG GTT TAT CGC GAC CTG GCT GCC GAT CCC ATC GCC CAC TTC TCC GCT GCC ACC CTG GTC GCT GCA GGC ACC CCT GAA GAC GTA CGC GAC 
GCG GCC CAG GCT TTC GAA GCC AAG CTG AGC GCC GAG ACG CTC GAA GAG TTC CTG GCT GCC GAT GGC GTG GTG ATT GGT GCG CCC ATG TAC AAC TTC ACC 
GTG CCG ACC CAG CTC AAA GCC TGG ATT GAC CGC GTA GCC GTC GCC GGC AAG ACC TTC CGC TAC ACC GAA GCC GGC CCG CAA GGC CTG AGC GGC GAC AAG 
AAA GTG GTG CTG GTT TCC ACT GCG GGT GGC CAG CAT GCT GGC CAG CCG ACT GGC GCC GGG CAT GAA GAC TTC CTG AAA GCG TTC CTG GGC TTC ATC GGG 
ATT ACT GAC CTG GAA ATC GTC CGG GCC CAC GGC CTG GAC TAT GGC CCG GAG CAG CGC AGC CAG GCG ATA GAT GCT GCA CGG GCG CAG ATT GCC AGC GAG 
CTG TTT GCG GCT GCC TGA

[Proposed] BBa_K1336002 / CotA Part-only sequence (1542bp)

Sequence given from Lisbon group directly.

ATG ACA CTT GAA AAA TTT GTG GAT GCT CTC CCA ATC CCA GAT ACA CTA AAG CCA GTA CAG CAA TCA AAA GAA AAA ACA TAC TAC GAA GTC ACC ATG GAG 
GAA TGC ACT CAT CAG CTC CAT CGC GAT CTC CCT CCA ACC CGC CTG TGG GGC TAC AAC GGC TTA TTT CCG GGA CCG ACC ATT GAG GTT AAA AGA AAT GAA 
AAC GTA TAT GTA AAA TGG ATG AAT AAC CTT CCT TCC ACG CAT TTC CTT CCG ATT GAT CAC ACC ATT CAT CAC AGT GAC AGC CAG CAT GAA GAG CCC GAG 
GTA AAG ACT GTT GTT CAT TTA CAC GGC GGC GTC ACG CCA GAT GAT AGT GAC GGG TAT CCG GAG GCT TGG TTT TCC AAA GAC TTT GAA CAA ACA GGA CCT 
TAT TTC AAA AGA GAG GTT TAT CAT TAT CCA AAC CAG CAG CGC GGG GCT ATA TTG TGG TAT CAC GAT CAC GCC ATG GCG CTC ACC AGG CTA AAT GTC TAT 
GCC GGA CTT GTC GGT GCA TAT ATC ATT CAT GAC CCA AAG GAA AAA CGC TTA AAA CTG CCT TCA GAC GAA TAC GAT GTG CCG CTT CTT ATC ACA GAC CGC 
ACG ATC AAT GAG GAT GGT TCT TTG TTT TAT CCG AGC GCA CCG GAA AAC CCT TCT CCG TCA CTG CCT AAT CCT TCA ATC GTT CCG GCT TTT TGC GGA GAA 
ACC ATA CTC GTC AAC GGG AAG GTA TGG CCA TAC TTG GAA GTC GAG CCA AGG AAA TAC CGA TTC CGT GTC ATC AAC GCC TCC AAT ACA AGA ACC TAT AAC 
CTG TCA CTC GAT AAT GGC GGA GAT TTT ATT CAG ATT GGT TCA GAT GGA GGG CTC CTG CCG CGA TCT GTT AAA CTG AAT TCT TTC AGC CTT GCG CCT GCT 
GAA CGT TAC GAT ATC ATC ATT GAC TTC ACA GCA TAT GAA GGA GAA TCG ATC ATT TTG GCA AAC AGC GCG GGC TGC GGC GGT GAC GTC AAT CCT GAA ACA 
GAT GCG AAT ATC ATG CAA TTC AGA GTC ACA AAA CCA TTG GCA CAA AAA GAC GAA AGC AGA AAG CCG AAG TAC CTC GCC TCA TAC CCT TCG GTA CAG CAT 
GAA AGA ATA CAA AAC ATC AGA ACG TTA AAA CTG GCA GGC ACC CAG GAC GAA TAC GGC AGA CCC GTC CTT CTG CTT AAT AAC AAA CGC TGG CAC GAT CCC 
GTC ACA GAA ACA CCA AAA GTC GGC ACA ACT GAA ATA TGG TCC ATT ATC AAC CCG ACA CGC GGA ACA CAT CCG ATC CAC CTG CAT CTA GTC TCC TTC CGT 
GTA TTA GAC CGG CGG CCG TTT GAT ATC GCC CGT TAT CAA GAA AGC GGG GAA TTG TCC TAT ACC GGT CCG GCT GTC CCG CCG CCG CCA AGT GAA AAG GGC 
TGG AAA GAC ACC ATT CAA GCG CAT GCA GGT GAA GTC CTG AGA ATC GCG GCG ACA TTC GGT CCG TAC AGC GGA CGA TAC GTA TGG CAT TGC CAT ATT CTA 
GAG CAT GAA GAC TAT GAC ATG ATG AGA CCG ATG GAT ATA ACT GAT CCC CAT AAA TAA

[New] BBa_K1336003 / BsDyP Part-only sequence (1251bp)

Sequence given from Lisbon group directly.

ATG AGC GAT GAA CAG AAA AAG CCA GAA CAA ATT CAC AGA CGG GAC ATT TTA AAA TGG GGA GCG ATG GCG GGG GCA GCC GTT GCG ATC GGT GCC AGC GGT 
CTC GGC GGT CTC GCT CCG CTT GTT CAG ACT GCG GCT AAG CCA TCG AAA AAG GAT GAA AAA GAG GAG GAG CAG ATT GTT CCG TTT TAC GGA AAG CAT CAG 
GCC GGA ATC ACA ACT GCC CAT CAG ACG TAT GTC TAT TTT GCT GCA CTG GAT GTA ACA GCA AAA GAC AAA AGC GAC ATC ATT ACG TTA TTT AGA AAC TGG 
ACA AGT CTC ACA CAG ATG CTG ACG TCT GGC AAG AAA ATG TCA GCG GAG CAG AGA AAT CAG TAT CTG CCG CCG CAG GAT ACA GGT GAG TCT GCT GAT TTA 
TCC CCT TCC AAC TTA ACG GTC ACA TTC GGA TTC GGG CCT GGC TTT TTT GAA AAA GAC GGA AAG GAC CGC TTC GGA CTG AAA AGC AAA AAA CCG AAG CAC 
CTA GCC GCT CTT CCA GCG ATG CCC AAT GAC AAC CTG GAT GAA AAG CAG GGA GGC GGG GAC ATC TGC ATT CAA GTG TGT GCA GAC GAC GAG CAA GTG GCG 
TTT CAC GCG CTG CGT AAC CTG CTA AAT CAA GCG GTC GGC ACT TGT GAG GTC CGT TTT GTG AAC AAA GGC TTT TTA AGC GGT GGA AAA AAT GGT GAA ACA 
CCG CGC AAC CTC TTC GGG TTT AAA GAT GGA ACA GGC AAC CAG AGC ACG AAG GAT GAC ACG TTG ATG AAC TCG ATC GTG TGG ATT CAG TCT GGT GAG CCT 
GAC TGG ATG ACG GGC GGC ACC TAT ATG GCT TTT CGG AAA ATC AAA ATG TTC CTT GAG GTA TGG GAC CGC TCT TCC CTC AAG GAC CAA GAG GAT ACC TTC 
GGC CGC AGA AAA AGC TCG GGG GCG CCT TTT GGG CAG AAA AAG GAA ACA GAT CCC GTG AAG CTG AAT CAA ATT CCG TCT AAT TCA CAC GTC AGT CTA GCG 
AAA TCT ACT GGG AAA CAA ATT TTG CGA AGA GCT TTC TCT TAC ACA GAA GGA CTT GAT CCG AAA ACC GGC TAT ATG GAT GCA GGT CTC CTG TTT ATC AGT 
TTT CAA AAA AAT CCC GAC AAT CAG TTT ATC CCG ATG CTG AAG GCT CTT TCA GCA AAG GAT GCG TTA AAC GAA TAT ACA CAA ACA ATC GGT TCT GCT TTA 
TAC GCA TGC CCA GGC GGC TGC AAA AAA GGA GAA TAT ATT GCC CAG CGT TTG CTG GAA TCA TAA

[Proposed] BBa_K1336004 / PpDyP Part-only sequence (864bp)

Sequence taken from Lisbon.

ATG CCG TTC CAG CAA GGT CTG CTT GCC ACC CCG GTG CCG GCC CAC GCC CGT CAC CTG TTC TTC ACC CTG CAG TCG CCC GAG GCG TTG CCT GCC GCG CTG 
GAT GCC TTG CTG CCG CAG GTC GAT GGC AAA CAG TTG CTG CTG GGC GTA GGC GCC CCC TTG GCC AAG GCC CTG GGC CGG GAA ATA CCA GGC CTG CGC CCG 
TTC CCG CTA CTG GAT GCC GCG GTT GAG AAC CCC AGC ACC CAA CAC GCC CTG TGG CTG TGG CTG CGT GGC GAC GAA CGT GGC GAC CTG CTG CTG CGC ACC 
CAG GCC CTG GAG CAG GCG CTG GCA CCC GCC CTG AGC CTG GCC GAC AGC GTT GAT GGC TTC CTG CAC CGG GGC GGC CAT GAC CTT ACC GGT TAT GAA GAC 
GGC ACC GAA AAC CCG ACA GAC GAA GAG GCG GTG CAA GCG GCC ATT GCC GCA GAT GGC TCC AGC TTT GCC GCG TTC CAG CTG TGG AAG CAC GAC CTG CAA 
TAC TTC AAG TCG CTG CCC CAG GCA GAC CAG GAC AAC ATC ATC GGC CGT CGC TTG AGC GAT AAC GAA GAA CTC GAC GAC GCC CCC GCG TCC GCG CAC GTC 
AAG CGC ACC GCC CAG GAA AGC TTT GAA CCC GAG GCG TTC ATG GTC CGT CGC TCG GTA TCC TGG GCC GAC CAA CGC GGC GCC GGC CTG GCT TTC GTC GCC 
CTG GGC AAG AGT TTC GAG GCA TTC GAG GTG CAG CTG CGG CGC ATG AGT GGC CTG GAA GAC GGC ATC ATC GAC GGG CTG TAC CGC TTC AGC CGC CCG CTG 
ACG GGG GGC TAC TAC TGG TGC CCG CCA ATG AGC GAG ACG GGT GTC GAT CTA AGC CCC TTG CTG CGG GCC TGA

[Improvement] BBa_K500000 / LiP Part-only sequence (1116bp)

Sequence taken from the initial page; not currently showing our improvements.

ATG GCT TTT AAA CAA TTA TTC GCC GCT ATT TCC TTG GCT TTG TCT TTG TCT GCT GCT AAT GCT GCT GCT GTT ATT GAA AAA AGA GCT ACT TGT TCC AAC 
GGT AAG ACT GTT GGT GAT GCT TGT TCT TGT GCT TGG TTT GAT GTT TTG GAC GAC ATC CAA CAA AAC TTG TTT CAT GGT GGT CAA TGT GGT GCT GAA GCT 
CAT GAA TCT ATC AGA TTG GTT TTC CAT GAC TCC ATT GCT ATT TCT CCA GCT ATG GAA GCT CAA GGT AAA TTT GGT GGA GGT GGT GCT GAT GGT TCC ATT 
ATG ATT TTC GAT GAT ATC GAA ACC GCC TTC CAT CCA AAT ATT GGT TTG GAC GAA ATC GTC AAG TTG CAA AAG CCA TTT GTT CAA AAG CAC GGT GTT ACT 
CCA GGT GAT TTT ATT GCT TTT GCT GGT GCT GTT GCT TTG TCT AAT TGT CCA GGT GCT CCA CAA ATG AAT TTC TTT ACT GGT AGA GCA CCA GCT ACT CAA 
CCA GCT CCA GAT GGT TTG GTT CCA GAA CCA TTT CAT ACC GTT GAC CAA ATC ATC AAC AGA GTT AAT GAC GCC GGT GAA TTT GAC GAA TTG GAA TTG GTT 
TGG ATG TTG TCT GCT CAT TCT GTT GCT GCT GTT AAT GAT GTT GAT CCA ACT GTT CAA GGT TTG CCA TTT GAT TCT ACC CCA GGT ATT TTC GAT TCC CAA 
TTC TTC GTT GAA ACC CAA TTG AGA GGT ACT GCT TTT CCA GGT TCT GGT GGT AAT CAA GGT GAA GTT GAA TCT CCA TTG CCA GGT GAA ATT AGA ATC CAA 
TCC GAT CAT ACC ATT GCC AGA GAT TCA AGA ACT GCT TGT GAA TGG CAA TCC TTC GTT AAC AAT CAA TCC AAG TTG GTT GAC GAC TTC CAA TTC ATT TTC 
TTG GCT TTG ACT CAA TTG GGT CAA GAT CCA AAT GCT ATG ACT GAT TGC TCC GAT GTT ATT CCA CAA TCT AAA CCT ATC CCA GGT AAC TTG CCA TTT TCA 
TTT TTT CCA GCT GGT AAG ACC ATC AAG GAT GTT GAA CAA GCT TGT GCT GAA ACT CCA TTT CCA ACT TTG ACT ACT TTG CCA GGT CCA GAA ACT TCT GTT 
CAA AGA ATA CCA CCA CCA CCA GGT GCT

[Improvement] BBa_K729006 / CueO Part-only sequence (1612bp)

Sequence taken from the initial page; not currently showing our improvements.

TTG ACA GCT AGC TCA GTC CTA GGT ATA ATG CTA GCT ACT AGA GAA AGA GGA GAA ATA CTA GAT GCA ACG TCG TGA TTT CTT AAA ATA TTC CGT CGC GCT 
GGG TGT GGC TTC GGC TTT GCC GCT GTG GAG CCG CGC AGT ATT TGC GGC AGA ACG CCC AAC GTT ACC GAT CCC TGA TTT GCT CAC GAC CGA TGC CCG TAA 
TCG CAT TCA GTT AAC TAT TGG CGC AGG CCA GTC CAC CTT TGG CGG GAA AAC TGC AAC TAC CTG GGG CTA TAA CGG CAA TCT GCT GGG GCC GGC GGT GAA 
ATT ACA GCG CGG CAA AGC GGT AAC GGT TGA TAT CTA CAA CCA ACT GAC GGA AGA GAC AAC GTT GCA CTG GCA CGG GCT GGA AGT ACC GGG TGA AGT CGA 
CGG CGG CCC GCA GGG AAT TAT TCC GCC AGG TGG CAA GCG CTC GGT GAC GTT GAA CGT TGA TCA ACC TGC CGC TAC CTG CTG GTT CCA TCC GCA TCA GCA 
CGG CAA AAC CGG GCG ACA GGT GGC GAT GGG GCT GGC TGG GCT GGT GGT GAT TGA AGA TGA CGA GAT CCT GAA ATT AAT GCT GCC AAA ACA GTG GGG TAT 
CGA TGA TGT TCC GGT GAT CGT TCA GGA TAA GAA ATT TAG CGC CGA CGG GCA GAT TGA TTA TCA ACT GGA TGT GAT GAC CGC CGC CGT GGG CTG GTT TGG 
CGA TAC GTT GCT GAC CAA CGG TGC AAT CTA CCC GCA ACA CGC TGC CCC GCG TGG TTG GCT GCG CCT GCG TTT GCT CAA TGG CTG TAA TGC CCG TTC GCT 
CAA TTT CGC CAC CAG CGA CAA TCG CCC GCT GTA TGT GAT TGC CAG CGA CGG TGG TCT GCT ACC TGA ACC AGT GAA GGT GAG CGA ACT GCC GGT GCT GAT 
GGG CGA GCG TTT TGA AGT GCT GGT GGA GGT TAA CGA TAA CAA ACC CTT TGA CCT GGT GAC GCT GCC GGT CAG CCA GAT GGG GAT GGC GAT TGC GCC GTT 
TGA TAA GCC TCA TCC GGT AAT GCG GAT TCA GCC GAT TGC TAT TAG TGC CTC CGG TGC TTT GCC AGA CAC ATT AAG TAG CCT GCC TGC GTT ACC TTC GCT 
GGA AGG GCT GAC GGT ACG CAA GCT GCA ACT CTC TAT GGA CCC GAT GCT CGA TAT GAT GGG GAT GCA GAT GCT AAT GGA GAA ATA TGG CGA TCA GGC GAT 
GGC CGG GAT GGA TCA CAG CCA GAT GAT GGG CCA TAT GGG GCA CGG CAA TAT GAA TCA TAT GAA CCA CGG CGG GAA GTT CGA TTT CCA CCA TGC CAA CAA 
AAT CAA CGG TCA GGC GTT TGA TAT GAA CAA GCC GAT GTT TGC GGC GGC GAA AGG GCA ATA CGA ACG TTG GGT TAT CTC TGG CGT GGG CGA CAT GAT GCT 
GCA TCC GTT CCA TAT CCA CGG CAC GCA GTT CCG TAT CTT GTC AGA AAA TGG CAA ACC GCC AGC GGC TCA TCG CGC GGG CTG GAA AGA TAC CGT TAA GGT 
AGA AGG TAA TGT CAG CGA AGT GCT GGT GAA GTT TAA TCA CGA TGC ACC GAA AGA ACA TGC TTA TAT GGC GCA CTG CCA TCT GCT GGA GCA TGA AGA TAC 
GGG GAT GAT GTT AGG GTT TAC GGT ATA A

[New] BBa_K1336005 / ispB Part-only sequence (562bp)

The first sequence below shows the "sense" ispB gene, but we use the second sequence, the Reverse Complement to design primers to produce an antisense fragment for gene knockdown (i.e. as RNAi).

"Sense" ispB sequence (972bp)

Black = region for PCR. Underlined CTG CAG = illegal PstI restriction site.

ATG AAT TTA GAA AAA ATC AAT GAG TTA ACC GCG CAA GAT ATG GCG GGT GTT AAT GCG GCA ATC CTT GAG CAG CTT AAT TCC GAC GTC CAA CTG ATC AAT 
CAG TTA GGC TAT TAC ATC GTC AGC GGC GGC GGT AAA CGT ATT CGT CCG ATG ATT GCT GTA CTG GCT GCA CGA GCT GTT GGC TAT GAG GGA AAT GCG CAT 
GTC ACC ATT GCT GCC CTG ATC GAG TTT ATC CAC ACG GCG ACT CTG CTA CAC GAC GAC GTT GTG GAT GAA TCA GAT ATG CGC AGG GGT AAA GCT ACC GCC 
AAC GCC GCA TTT GGC AAT GCC GCC AGC GTG CTG GTA GGC GAT TTT ATT TAT ACC CGC GCT TTC CAG ATG ATG ACC AGC CTC GGT TCG CTC AAA GTG CTG 
GAA GTC ATG TCA GAA GCC GTA AAC GTC ATC GCA GAA GGT GAA GTT CTG CAA CTG ATG AAC GTT AAC GAT CCG GAC ATC ACT GAA GAA AAC TAC ATG CGC 
GTT ATC TAT AGC AAA ACC GCG CGT CTG TTT GAG GCT GCC GCG CAG TGT TCC GGG ATT CTG GCT GGC TGT ACG CCG GAG GAG GAG AAA GGC CTG CAG GAT 
TAT GGG CGC TAT CTC GGC ACT GCT TTC CAG TTG ATC GAC GAT TTA CTC GAT TAC AAT GCC GAT GGC GAA CAG TTA GGT AAA AAT GTC GGC GAC GAT CTG 
AAC GAA GGT AAA CCG ACG CTG CCG CTG CTG CAT GCG ATG CAT CAT GGC ACA CCA GAA CAG GCA CAG ATG ATC CGT ACC GCC ATC GAA CAG GGT AAC GGT 
CGC CAT CTT CTG GAA CCG GTT CTG GAA GCA ATG AAC GCT TGT GGA TCT CTT GAA TGG ACG CGT CAG CGT GCC GAG GAA GAA GCA GAC AAA GCC ATC GCA 
GCG TTA CAG GTG CTC CCG GAC ACC CCT TGG CGA GAA GCA CTC ATC GGC CTC GCG CAC ATC GCT GTT CAA CGC GAT CGT TAA

"Antisense" ispB sequence (972bp)

To create the RNAi/antisense fragment, we first get the reverse complement of the full ispB gene, before designing the primers to amplify the black region.

TTA ACG ATC GCG TTG AAC AGC GAT GTG CGC GAG GCC GAT GAG TGC TTC TCG CCA AGG GGT GTC CGG GAG CAC CTG TAA CGC TGC GAT GGC TTT GTC TGC 
TTC TTC CTC GGC ACG CTG ACG CGT CCA TTC AAG AGA TCC ACA AGC GTT CAT TGC TTC CAG AAC CGG TTC CAG AAG ATG GCG ACC GTT ACC CTG TTC GAT 
GGC GGT ACG GAT CAT CTG TGC CTG TTC TGG TGT GCC ATG ATG CAT CGC ATG CAG CAG CGG CAG CGT CGG TTT ACC TTC GTT CAG ATC GTC GCC GAC ATT 
TTT ACC TAA CTG TTC GCC ATC GGC ATT GTA ATC GAG TAA ATC GTC GAT CAA CTG GAA AGC AGT GCC GAG ATA GCG CCC ATA ATC CTG CAG GCC TTT CTC 
CTC CTC CGG CGT ACA GCC AGC CAG AAT CCC GGA ACA CTG CGC GGC AGC CTC AAA CAG ACG CGC GGT TTT GCT ATA GAT AAC GCG CAT GTA GTT TTC TTC 
AGT GAT GTC CGG ATC GTT AAC GTT CAT CAG TTG CAG AAC TTC ACC TTC TGC GAT GAC GTT TAC GGC TTC TGA CAT GAC TTC CAG CAC TTT GAG CGA ACC 
GAG GCT GGT CAT CAT CTG GAA AGC GCG GGT ATA AAT AAA ATC GCC TAC CAG CAC GCT GGC GGC ATT GCC AAA TGC GGC GTT GGC GGT AGC TTT ACC CCT 
GCG CAT ATC TGA TTC ATC CAC AAC GTC GTC GTG TAG CAG AGT CGC CGT GTG GAT AAA CTC GAT CAG GGC AGC AAT GGT GAC ATG CGC ATT TCC CTC ATA 
GCC AAC AGC TCG TGC AGC CAG TAC AGC AAT CAT CGG ACG AAT ACG TTT ACC GCC GCC GCT GAC GAT GTA ATA GCC TAA CTG ATT GAT CAG TTG GAC GTC 
GGA ATT AAG CTG CTC AAG GAT TGC CGC ATT AAC ACC CGC CAT ATC TTG CGC GGT TAA CTC ATT GAT TTT TTC TAA ATT CAT

"Antisense" RNAi ispB sequence PCR fragment (562bp)

...

  G GCC TTT CTC CTC CTC CGG CGT ACA GCC AGC CAG AAT CCC GGA ACA CTG CGC GGC AGC CTC AAA CAG ACG CGC GGT TTT GCT ATA GAT AAC GCG CAT 
GTA GTT TTC TTC AGT GAT GTC CGG ATC GTT AAC GTT CAT CAG TTG CAG AAC TTC ACC TTC TGC GAT GAC GTT TAC GGC TTC TGA CAT GAC TTC CAG CAC 
TTT GAG CGA ACC GAG GCT GGT CAT CAT CTG GAA AGC GCG GGT ATA AAT AAA ATC GCC TAC CAG CAC GCT GGC GGC ATT GCC AAA TGC GGC GTT GGC GGT 
AGC TTT ACC CCT GCG CAT ATC TGA TTC ATC CAC AAC GTC GTC GTG TAG CAG AGT CGC CGT GTG GAT AAA CTC GAT CAG GGC AGC AAT GGT GAC ATG CGC 
ATT TCC CTC ATA GCC AAC AGC TCG TGC AGC CAG TAC AGC AAT CAT CGG ACG AAT ACG TTT ACC GCC GCC GCT GAC GAT GTA ATA GCC TAA CTG ATT GAT 
CAG TTG GAC GTC GGA ATT AAG CTG CTC AAG GAT TGC CGC ATT AAC ACC CGC CAT ATC TTG CGC GGT TAA

[New] BBa_K1336006 / LacI Expression Cassette + ispB Part-only sequence (Xbp)

LacI EC + K1336005.

LacI EC + "Antisense" ispB Device sequence

LACIEC NEEDED + PREFIX NEEDED + TTA ACG ATC GCG TTG AAC AGC GAT GTG CGC GAG GCC GAT GAG TGC TTC TCG CCA AGG GGT GTC CGG GAG CAC CTG TAA CGC TGC GAT GGC TTT GTC TGC 
TTC TTC CTC GGC ACG CTG ACG CGT CCA TTC AAG AGA TCC ACA AGC GTT CAT TGC TTC CAG AAC CGG TTC CAG AAG ATG GCG ACC GTT ACC CTG TTC GAT 
GGC GGT ACG GAT CAT CTG TGC CTG TTC TGG TGT GCC ATG ATG CAT CGC ATG CAG CAG CGG CAG CGT CGG TTT ACC TTC GTT CAG ATC GTC GCC GAC ATT 
TTT ACC TAA CTG TTC GCC ATC GGC ATT GTA ATC GAG TAA ATC GTC GAT CAA CTG GAA AGC AGT GCC GAG ATA GCG CCC ATA ATC CTG CAG GCC TTT CTC 
CTC CTC CGG CGT ACA GCC AGC CAG AAT CCC GGA ACA CTG CGC GGC AGC CTC AAA CAG ACG CGC GGT TTT GCT ATA GAT AAC GCG CAT GTA GTT TTC TTC 
AGT GAT GTC CGG ATC GTT AAC GTT CAT CAG TTG CAG AAC TTC ACC TTC TGC GAT GAC GTT TAC GGC TTC TGA CAT GAC TTC CAG CAC TTT GAG CGA ACC 
GAG GCT GGT CAT CAT CTG GAA AGC GCG GGT ATA AAT AAA ATC GCC TAC CAG CAC GCT GGC GGC ATT GCC AAA TGC GGC GTT GGC GGT AGC TTT ACC CCT 
GCG CAT ATC TGA TTC ATC CAC AAC GTC GTC GTG TAG CAG AGT CGC CGT GTG GAT AAA CTC GAT CAG GGC AGC AAT GGT GAC ATG CGC ATT TCC CTC ATA 
GCC AAC AGC TCG TGC AGC CAG TAC AGC AAT CAT CGG ACG AAT ACG TTT ACC GCC GCC GCT GAC GAT GTA ATA GCC TAA CTG ATT GAT CAG TTG GAC GTC 
GGA ATT AAG CTG CTC AAG GAT TGC CGC ATT AAC ACC CGC CAT ATC TTG CGC GGT TAA CTC ATT GAT TTT TTC TAA ATT CAT + SUFFIX NEEDED

[New] BBa_K1336007 / LacI Expression Cassette + BsDyP Part-only sequence (XXXbp)

LacI EC + K1336003.

LacI EC + BsDyP Device sequence

1 100 199 298 397 496 595 694 793 892	LACIEC NEEDED + PREFIX NEEDED + [[BsDyP NEEDED]] + SUFFIX NEEDED

Plasmid Backbone / Vectors

pET-21a (+) sequence (5443bp) [Link, see DT]

...

ATC CGG ATA TAG TTC CTC CTT TCA GCA AAA AAC CCC TCA AGA CCC GTT TAG AGG CCC CAA GGG GTT ATG CTA GTT ATT GCT CAG CGG TGG CAG CAG CCA 
ACT CAG CTT CCT TTC GGG CTT TGT TAG CAG CCG GAT CTC AGT GGT GGT GGT GGT GGT GCT CGA GTG CGG CCG CAA GCT TGT CGA CGG AGC TCG AAT TCG 
GAT CCG CGA CCC ATT TGC TGT CCA CCA GTC ATG CTA GCC ATA TGT ATA TCT CCT TCT TAA AGT TAA ACA AAA TTA TTT CTA GAG GGG AAT TGT TAT CCG 
CTC ACA ATT CCC CTA TAG TGA GTC GTA TTA ATT TCG CGG GAT CGA GAT CTC GAT CCT CTA CGC CGG ACG CAT CGT GGC CGG CAT CAC CGG CGC CAC AGG 
TGC GGT TGC TGG CGC CTA TAT CGC CGA CAT CAC CGA TGG GGA AGA TCG GGC TCG CCA CTT CGG GCT CAT GAG CGC TTG TTT CGG CGT GGG TAT GGT GGC 
AGG CCC CGT GGC CGG GGG ACT GTT GGG CGC CAT CTC CTT GCA TGC ACC ATT CCT TGC GGC GGC GGT GCT CAA CGG CCT CAA CCT ACT ACT GGG CTG CTT 
CCT AAT GCA GGA GTC GCA TAA GGG AGA GCG TCG AGA TCC CGG ACA CCA TCG AAT GGC GCA AAA CCT TTC GCG GTA TGG CAT GAT AGC GCC CGG AAG AGA 
GTC AAT TCA GGG TGG TGA ATG TGA AAC CAG TAA CGT TAT ACG ATG TCG CAG AGT ATG CCG GTG TCT CTT ATC AGA CCG TTT CCC GCG TGG TGA ACC AGG 
CCA GCC ACG TTT CTG CGA AAA CGC GGG AAA AAG TGG AAG CGG CGA TGG CGG AGC TGA ATT ACA TTC CCA ACC GCG TGG CAC AAC AAC TGG CGG GCA AAC 
AGT CGT TGC TGA TTG GCG TTG CCA CCT CCA GTC TGG CCC TGC ACG CGC CGT CGC AAA TTG TCG CGG CGA TTA AAT CTC GCG CCG ATC AAC TGG GTG CCA 
GCG TGG TGG TGT CGA TGG TAG AAC GAA GCG GCG TCG AAG CCT GTA AAG CGG CGG TGC ACA ATC TTC TCG CGC AAC GCG TCA GTG GGC TGA TCA TTA ACT 
ATC CGC TGG ATG ACC AGG ATG CCA TTG CTG TGG AAG CTG CCT GCA CTA ATG TTC CGG CGT TAT TTC TTG ATG TCT CTG ACC AGA CAC CCA TCA ACA GTA 
TTA TTT TCT CCC ATG AAG ACG GTA CGC GAC TGG GCG TGG AGC ATC TGG TCG CAT TGG GTC ACC AGC AAA TCG CGC TGT TAG CGG GCC CAT TAA GTT CTG 
TCT CGG CGC GTC TGC GTC TGG CTG GCT GGC ATA AAT ATC TCA CTC GCA ATC AAA TTC AGC CGA TAG CGG AAC GGG AAG GCG ACT GGA GTG CCA TGT CCG 
GTT TTC AAC AAA CCA TGC AAA TGC TGA ATG AGG GCA TCG TTC CCA CTG CGA TGC TGG TTG CCA ACG ATC AGA TGG CGC TGG GCG CAA TGC GCG CCA TTA 
CCG AGT CCG GGC TGC GCG TTG GTG CGG ATA TCT CGG TAG TGG GAT ACG ACG ATA CCG AAG ACA GCT CAT GTT ATA TCC CGC CGT TAA CCA CCA TCA AAC 
AGG ATT TTC GCC TGC TGG GGC AAA CCA GCG TGG ACC GCT TGC TGC AAC TCT CTC AGG GCC AGG CGG TGA AGG GCA ATC AGC TGT TGC CCG TCT CAC TGG 
TGA AAA GAA AAA CCA CCC TGG CGC CCA ATA CGC AAA CCG CCT CTC CCC GCG CGT TGG CCG ATT CAT TAA TGC AGC TGG CAC GAC AGG TTT CCC GAC TGG 
AAA GCG GGC AGT GAG CGC AAC GCA ATT AAT GTA AGT TAG CTC ACT CAT TAG GCA CCG GGA TCT CGA CCG ATG CCC TTG AGA GCC TTC AAC CCA GTC AGC 
TCC TTC CGG TGG GCG CGG GGC ATG ACT ATC GTC GCC GCA CTT ATG ACT GTC TTC TTT ATC ATG CAA CTC GTA GGA CAG GTG CCG GCA GCG CTC TGG GTC 
ATT TTC GGC GAG GAC CGC TTT CGC TGG AGC GCG ACG ATG ATC GGC CTG TCG CTT GCG GTA TTC GGA ATC TTG CAC GCC CTC GCT CAA GCC TTC GTC ACT 
GGT CCC GCC ACC AAA CGT TTC GGC GAG AAG CAG GCC ATT ATC GCC GGC ATG GCG GCC CCA CGG GTG CGC ATG ATC GTG CTC CTG TCG TTG AGG ACC CGG 
CTA GGC TGG CGG GGT TGC CTT ACT GGT TAG CAG AAT GAA TCA CCG ATA CGC GAG CGA ACG TGA AGC GAC TGC TGC TGC AAA ACG TCT GCG ACC TGA GCA 
ACA ACA TGA ATG GTC TTC GGT TTC CGT GTT TCG TAA AGT CTG GAA ACG CGG AAG TCA GCG CCC TGC ACC ATT ATG TTC CGG ATC TGC ATC GCA GGA TGC 
TGC TGG CTA CCC TGT GGA ACA CCT ACA TCT GTA TTA ACG AAG CGC TGG CAT TGA CCC TGA GTG ATT TTT CTC TGG TCC CGC CGC ATC CAT ACC GCC AGT 
TGT TTA CCC TCA CAA CGT TCC AGT AAC CGG GCA TGT TCA TCA TCA GTA ACC CGT ATC GTG AGC ATC CTC TCT CGT TTC ATC GGT ATC ATT ACC CCC ATG 
AAC AGA AAT CCC CCT TAC ACG GAG GCA TCA GTG ACC AAA CAG GAA AAA ACC GCC CTT AAC ATG GCC CGC TTT ATC AGA AGC CAG ACA TTA ACG CTT CTG 
GAG AAA CTC AAC GAG CTG GAC GCG GAT GAA CAG GCA GAC ATC TGT GAA TCG CTT CAC GAC CAC GCT GAT GAG CTT TAC CGC AGC TGC CTC GCG CGT TTC 
GGT GAT GAC GGT GAA AAC CTC TGA CAC ATG CAG CTC CCG GAG ACG GTC ACA GCT TGT CTG TAA GCG GAT GCC GGG AGC AGA CAA GCC CGT CAG GGC GCG 
TCA GCG GGT GTT GGC GGG TGT CGG GGC GCA GCC ATG ACC CAG TCA CGT AGC GAT AGC GGA GTG TAT ACT GGC TTA ACT ATG CGG CAT CAG AGC AGA TTG 
TAC TGA GAG TGC ACC ATA TAT GCG GTG TGA AAT ACC GCA CAG ATG CGT AAG GAG AAA ATA CCG CAT CAG GCG CTC TTC CGC TTC CTC GCT CAC TGA CTC 
GCT GCG CTC GGT CGT TCG GCT GCG GCG AGC GGT ATC AGC TCA CTC AAA GGC GGT AAT ACG GTT ATC CAC AGA ATC AGG GGA TAA CGC AGG AAA GAA CAT 
GTG AGC AAA AGG CCA GCA AAA GGC CAG GAA CCG TAA AAA GGC CGC GTT GCT GGC GTT TTT CCA TAG GCT CCG CCC CCC TGA CGA GCA TCA CAA AAA TCG 
ACG CTC AAG TCA GAG GTG GCG AAA CCC GAC AGG ACT ATA AAG ATA CCA GGC GTT TCC CCC TGG AAG CTC CCT CGT GCG CTC TCC TGT TCC GAC CCT GCC 
GCT TAC CGG ATA CCT GTC CGC CTT TCT CCC TTC GGG AAG CGT GGC GCT TTC TCA TAG CTC ACG CTG TAG GTA TCT CAG TTC GGT GTA GGT CGT TCG CTC 
CAA GCT GGG CTG TGT GCA CGA ACC CCC CGT TCA GCC CGA CCG CTG CGC CTT ATC CGG TAA CTA TCG TCT TGA GTC CAA CCC GGT AAG ACA CGA CTT ATC 
GCC ACT GGC AGC AGC CAC TGG TAA CAG GAT TAG CAG AGC GAG GTA TGT AGG CGG TGC TAC AGA GTT CTT GAA GTG GTG GCC TAA CTA CGG CTA CAC TAG 
AAG GAC AGT ATT TGG TAT CTG CGC TCT GCT GAA GCC AGT TAC CTT CGG AAA AAG AGT TGG TAG CTC TTG ATC CGG CAA ACA AAC CAC CGC TGG TAG CGG 
TGG TTT TTT TGT TTG CAA GCA GCA GAT TAC GCG CAG AAA AAA AGG ATC TCA AGA AGA TCC TTT GAT CTT TTC TAC GGG GTC TGA CGC TCA GTG GAA CGA 
AAA CTC ACG TTA AGG GAT TTT GGT CAT GAG ATT ATC AAA AAG GAT CTT CAC CTA GAT CCT TTT AAA TTA AAA ATG AAG TTT TAA ATC AAT CTA AAG TAT 
ATA TGA GTA AAC TTG GTC TGA CAG TTA CCA ATG CTT AAT CAG TGA GGC ACC TAT CTC AGC GAT CTG TCT ATT TCG TTC ATC CAT AGT TGC CTG ACT CCC 
CGT CGT GTA GAT AAC TAC GAT ACG GGA GGG CTT ACC ATC TGG CCC CAG TGC TGC AAT GAT ACC GCG AGA CCC ACG CTC ACC GGC TCC AGA TTT ATC AGC 
AAT AAA CCA GCC AGC CGG AAG GGC CGA GCG CAG AAG TGG TCC TGC AAC TTT ATC CGC CTC CAT CCA GTC TAT TAA TTG TTG CCG GGA AGC TAG AGT AAG 
TAG TTC GCC AGT TAA TAG TTT GCG CAA CGT TGT TGC CAT TGC TGC AGG CAT CGT GGT GTC ACG CTC GTC GTT TGG TAT GGC TTC ATT CAG CTC CGG TTC 
CCA ACG ATC AAG GCG AGT TAC ATG ATC CCC CAT GTT GTG CAA AAA AGC GGT TAG CTC CTT CGG TCC TCC GAT CGT TGT CAG AAG TAA GTT GGC CGC AGT 
GTT ATC ACT CAT GGT TAT GGC AGC ACT GCA TAA TTC TCT TAC TGT CAT GCC ATC CGT AAG ATG CTT TTC TGT GAC TGG TGA GTA CTC AAC CAA GTC ATT 
CTG AGA ATA GTG TAT GCG GCG ACC GAG TTG CTC TTG CCC GGC GTC AAT ACG GGA TAA TAC CGC GCC ACA TAG CAG AAC TTT AAA AGT GCT CAT CAT TGG 
AAA ACG TTC TTC GGG GCG AAA ACT CTC AAG GAT CTT ACC GCT GTT GAG ATC CAG TTC GAT GTA ACC CAC TCG TGC ACC CAA CTG ATC TTC AGC ATC TTT 
TAC TTT CAC CAG CGT TTC TGG GTG AGC AAA AAC AGG AAG GCA AAA TGC CGC AAA AAA GGG AAT AAG GGC GAC ACG GAA ATG TTG AAT ACT CAT ACT CTT 
CCT TTT TCA ATA TTA TTG AAG CAT TTA TCA GGG TTA TTG TCT CAT GAG CGG ATA CAT ATT TGA ATG TAT TTA GAA AAA TAA ACA AAT AGG GGT TCC GCG 
CAC ATT TCC CCG AAA AGT GCC ACC TGA AAT TGT AAA CGT TAA TAT TTT GTT AAA ATT CGC GTT AAA TTT TTG TTA AAT CAG CTC ATT TTT TAA CCA ATA 
GGC CGA AAT CGG CAA AAT CCC TTA TAA ATC AAA AGA ATA GAC CGA GAT AGG GTT GAG TGT TGT TCC AGT TTG GAA CAA GAG TCC ACT ATT AAA GAA CGT 
GGA CTC CAA CGT CAA AGG GCG AAA AAC CGT CTA TCA GGG CGA TGG CCC ACT ACG TGA ACC ATC ACC CTA ATC AAG TTT TTT GGG GTC GAG GTG CCG TAA 
AGC ACT AAA TCG GAA CCC TAA AGG GAG CCC CCG ATT TAG AGC TTG ACG GGG AAA GCC GGC GAA CGT GGC GAG AAA GGA AGG GAA GAA AGC GAA AGG AGC 
GGG CGC TAG GGC GCT GGC AAG TGT AGC GGT CAC GCT GCG CGT AAC CAC CAC ACC CGC CGC GCT TAA TGC GCC GCT ACA GGG CGC GTC CCA TTC GCC A

pSB1C3 Part-only sequence (2070bp)

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TAC TAG TAG CGG CCG CTG CAG TCC GGC AAA AAA GGG CAA GGT GTC ACC ACC CTG CCC TTT TTC TTT AAA ACC GAA AAG ATT ACT TCG CGT TAT GCA GGC 
TTC CTC GCT CAC TGA CTC GCT GCG CTC GGT CGT TCG GCT GCG GCG AGC GGT ATC AGC TCA CTC AAA GGC GGT AAT ACG GTT ATC CAC AGA ATC AGG GGA 
TAA CGC AGG AAA GAA CAT GTG AGC AAA AGG CCA GCA AAA GGC CAG GAA CCG TAA AAA GGC CGC GTT GCT GGC GTT TTT CCA CAG GCT CCG CCC CCC TGA 
CGA GCA TCA CAA AAA TCG ACG CTC AAG TCA GAG GTG GCG AAA CCC GAC AGG ACT ATA AAG ATA CCA GGC GTT TCC CCC TGG AAG CTC CCT CGT GCG CTC 
TCC TGT TCC GAC CCT GCC GCT TAC CGG ATA CCT GTC CGC CTT TCT CCC TTC GGG AAG CGT GGC GCT TTC TCA TAG CTC ACG CTG TAG GTA TCT CAG TTC 
GGT GTA GGT CGT TCG CTC CAA GCT GGG CTG TGT GCA CGA ACC CCC CGT TCA GCC CGA CCG CTG CGC CTT ATC CGG TAA CTA TCG TCT TGA GTC CAA CCC 
GGT AAG ACA CGA CTT ATC GCC ACT GGC AGC AGC CAC TGG TAA CAG GAT TAG CAG AGC GAG GTA TGT AGG CGG TGC TAC AGA GTT CTT GAA GTG GTG GCC 
TAA CTA CGG CTA CAC TAG AAG AAC AGT ATT TGG TAT CTG CGC TCT GCT GAA GCC AGT TAC CTT CGG AAA AAG AGT TGG TAG CTC TTG ATC CGG CAA ACA 
AAC CAC CGC TGG TAG CGG TGG TTT TTT TGT TTG CAA GCA GCA GAT TAC GCG CAG AAA AAA AGG ATC TCA AGA AGA TCC TTT GAT CTT TTC TAC GGG GTC 
TGA CGC TCA GTG GAA CGA AAA CTC ACG TTA AGG GAT TTT GGT CAT GAG ATT ATC AAA AAG GAT CTT CAC CTA GAT CCT TTT AAA TTA AAA ATG AAG TTT 
TAA ATC AAT CTA AAG TAT ATA TGA GTA AAC TTG GTC TGA CAG CTC GAG GCT TGG ATT CTC ACC AAT AAA AAA CGC CCG GCG GCA ACC GAG CGT TCT GAA 
CAA ATC CAG ATG GAG TTC TGA GGT CAT TAC TGG ATC TAT CAA CAG GAG TCC AAG CGA GCT CGA TAT CAA ATT ACG CCC CGC CCT GCC ACT CAT CGC AGT 
ACT GTT GTA ATT CAT TAA GCA TTC TGC CGA CAT GGA AGC CAT CAC AAA CGG CAT GAT GAA CCT GAA TCG CCA GCG GCA TCA GCA CCT TGT CGC CTT GCG 
TAT AAT ATT TGC CCA TGG TGA AAA CGG GGG CGA AGA AGT TGT CCA TAT TGG CCA CGT TTA AAT CAA AAC TGG TGA AAC TCA CCC AGG GAT TGG CTG AGA 
CGA AAA ACA TAT TCT CAA TAA ACC CTT TAG GGA AAT AGG CCA GGT TTT CAC CGT AAC ACG CCA CAT CTT GCG AAT ATA TGT GTA GAA ACT GCC GGA AAT 
CGT CGT GGT ATT CAC TCC AGA GCG ATG AAA ACG TTT CAG TTT GCT CAT GGA AAA CGG TGT AAC AAG GGT GAA CAC TAT CCC ATA TCA CCA GCT CAC CGT 
CTT TCA TTG CCA TAC GAA ATT CCG GAT GAG CAT TCA TCA GGC GGG CAA GAA TGT GAA TAA AGG CCG GAT AAA ACT TGT GCT TAT TTT TCT TTA CGG TCT 
TTA AAA AGG CCG TAA TAT CCA GCT GAA CGG TCT GGT TAT AGG TAC ATT GAG CAA CTG ACT GAA ATG CCT CAA AAT GTT CTT TAC GAT GCC ATT GGG ATA 
TAT CAA CGG TGG TAT ATC CAG TGA TTT TTT TCT CCA TTT TAG CTT CCT TAG CTC CTG AAA ATC TCG ATA ACT CAA AAA ATA CGC CCG GTA GTG ATC TTA 
TTT CAT TAT GGT GAA AGT TGG AAC CTC TTA CGT GCC CGA TCA ACT CGA GTG CCA CCT GAC GTC TAA GAA ACC ATT ATT ATC ATG ACA TTA ACC TAT AAA 
AAT AGG CGT ATC ACG AGG CAG AAT TTC AGA TAA AAA AAA TCC TTA GCT TTC GCT AAG GAT GAT TTC TGG AAT TCG CGG CCG CTT CTA GAG

pSB1A3 Part-only sequence (2155bp)

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TAC TAG TAG CGG CCG CTG CAG TCC GGC AAA AAA GGG CAA GGT GTC ACC ACC CTG CCC TTT TTC TTT AAA ACC GAA AAG ATT ACT TCG CGT TAT GCA GGC 
TTC CTC GCT CAC TGA CTC GCT GCG CTC GGT CGT TCG GCT GCG GCG AGC GGT ATC AGC TCA CTC AAA GGC GGT AAT ACG GTT ATC CAC AGA ATC AGG GGA 
TAA CGC AGG AAA GAA CAT GTG AGC AAA AGG CCA GCA AAA GGC CAG GAA CCG TAA AAA GGC CGC GTT GCT GGC GTT TTT CCA CAG GCT CCG CCC CCC TGA 
CGA GCA TCA CAA AAA TCG ACG CTC AAG TCA GAG GTG GCG AAA CCC GAC AGG ACT ATA AAG ATA CCA GGC GTT TCC CCC TGG AAG CTC CCT CGT GCG CTC 
TCC TGT TCC GAC CCT GCC GCT TAC CGG ATA CCT GTC CGC CTT TCT CCC TTC GGG AAG CGT GGC GCT TTC TCA TAG CTC ACG CTG TAG GTA TCT CAG TTC 
GGT GTA GGT CGT TCG CTC CAA GCT GGG CTG TGT GCA CGA ACC CCC CGT TCA GCC CGA CCG CTG CGC CTT ATC CGG TAA CTA TCG TCT TGA GTC CAA CCC 
GGT AAG ACA CGA CTT ATC GCC ACT GGC AGC AGC CAC TGG TAA CAG GAT TAG CAG AGC GAG GTA TGT AGG CGG TGC TAC AGA GTT CTT GAA GTG GTG GCC 
TAA CTA CGG CTA CAC TAG AAG AAC AGT ATT TGG TAT CTG CGC TCT GCT GAA GCC AGT TAC CTT CGG AAA AAG AGT TGG TAG CTC TTG ATC CGG CAA ACA 
AAC CAC CGC TGG TAG CGG TGG TTT TTT TGT TTG CAA GCA GCA GAT TAC GCG CAG AAA AAA AGG ATC TCA AGA AGA TCC TTT GAT CTT TTC TAC GGG GTC 
TGA CGC TCA GTG GAA CGA AAA CTC ACG TTA AGG GAT TTT GGT CAT GAG ATT ATC AAA AAG GAT CTT CAC CTA GAT CCT TTT AAA TTA AAA ATG AAG TTT 
TAA ATC AAT CTA AAG TAT ATA TGA GTA AAC TTG GTC TGA CAG TTA CCA ATG CTT AAT CAG TGA GGC ACC TAT CTC AGC GAT CTG TCT ATT TCG TTC ATC 
CAT AGT TGC CTG ACT CCC CGT CGT GTA GAT AAC TAC GAT ACG GGA GGG CTT ACC ATC TGG CCC CAG TGC TGC AAT GAT ACC GCG AGA CCC ACG CTC ACC 
GGC TCC AGA TTT ATC AGC AAT AAA CCA GCC AGC CGG AAG GGC CGA GCG CAG AAG TGG TCC TGC AAC TTT ATC CGC CTC CAT CCA GTC TAT TAA TTG TTG 
CCG GGA AGC TAG AGT AAG TAG TTC GCC AGT TAA TAG TTT GCG CAA CGT TGT TGC CAT TGC TAC AGG CAT CGT GGT GTC ACG CTC GTC GTT TGG TAT GGC 
TTC ATT CAG CTC CGG TTC CCA ACG ATC AAG GCG AGT TAC ATG ATC CCC CAT GTT GTG CAA AAA AGC GGT TAG CTC CTT CGG TCC TCC GAT CGT TGT CAG 
AAG TAA GTT GGC CGC AGT GTT ATC ACT CAT GGT TAT GGC AGC ACT GCA TAA TTC TCT TAC TGT CAT GCC ATC CGT AAG ATG CTT TTC TGT GAC TGG TGA 
GTA CTC AAC CAA GTC ATT CTG AGA ATA GTG TAT GCG GCG ACC GAG TTG CTC TTG CCC GGC GTC AAT ACG GGA TAA TAC CGC GCC ACA TAG CAG AAC TTT 
AAA AGT GCT CAT CAT TGG AAA ACG TTC TTC GGG GCG AAA ACT CTC AAG GAT CTT ACC GCT GTT GAG ATC CAG TTC GAT ATA ACC CAC TCG TGC ACC CAA 
CTG ATC TTC AGC ATC TTT TAC TTT CAC CAG CGT TTC TGG GTG AGC AAA AAC AGG AAG GCA AAA TGC CGC AAA AAA GGG AAT AAG GGC GAC ACG GAA ATG 
TTG AAT ACT CAT ACT CTT CCT TTT TCA ATA TTA TTG AAG CAT TTA TCA GGG TTA TTG TCT CAT GAG CGG ATA CAT ATT TGA ATG TAT TTA GAA AAA TAA 
ACA AAT AGG GGT TCC GCG CAC ATT TCC CCG AAA AGT GCC ACC TGA CGT CTA AGA AAC CAT TAT TAT CAT GAC ATT AAC CTA TAA AAA TAG GCG TAT CAC 
GAG GCA GAA TTT CAG ATA AAA AAA ATC CTT AGC TTT CGC TAA GGA TGA TTT CTG GAA TTC GCG GCC GCT TCT AGA G

pSB1K3 Part-only sequence (2204bp)

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TAC TAG TAG CGG CCG CTG CAG TCC GGC AAA AAA GGG CAA GGT GTC ACC ACC CTG CCC TTT TTC TTT AAA ACC GAA AAG ATT ACT TCG CGT TAT GCA GGC 
TTC CTC GCT CAC TGA CTC GCT GCG CTC GGT CGT TCG GCT GCG GCG AGC GGT ATC AGC TCA CTC AAA GGC GGT AAT ACG GTT ATC CAC AGA ATC AGG GGA 
TAA CGC AGG AAA GAA CAT GTG AGC AAA AGG CCA GCA AAA GGC CAG GAA CCG TAA AAA GGC CGC GTT GCT GGC GTT TTT CCA CAG GCT CCG CCC CCC TGA 
CGA GCA TCA CAA AAA TCG ACG CTC AAG TCA GAG GTG GCG AAA CCC GAC AGG ACT ATA AAG ATA CCA GGC GTT TCC CCC TGG AAG CTC CCT CGT GCG CTC 
TCC TGT TCC GAC CCT GCC GCT TAC CGG ATA CCT GTC CGC CTT TCT CCC TTC GGG AAG CGT GGC GCT TTC TCA TAG CTC ACG CTG TAG GTA TCT CAG TTC 
GGT GTA GGT CGT TCG CTC CAA GCT GGG CTG TGT GCA CGA ACC CCC CGT TCA GCC CGA CCG CTG CGC CTT ATC CGG TAA CTA TCG TCT TGA GTC CAA CCC 
GGT AAG ACA CGA CTT ATC GCC ACT GGC AGC AGC CAC TGG TAA CAG GAT TAG CAG AGC GAG GTA TGT AGG CGG TGC TAC AGA GTT CTT GAA GTG GTG GCC 
TAA CTA CGG CTA CAC TAG AAG AAC AGT ATT TGG TAT CTG CGC TCT GCT GAA GCC AGT TAC CTT CGG AAA AAG AGT TGG TAG CTC TTG ATC CGG CAA ACA 
AAC CAC CGC TGG TAG CGG TGG TTT TTT TGT TTG CAA GCA GCA GAT TAC GCG CAG AAA AAA AGG ATC TCA AGA AGA TCC TTT GAT CTT TTC TAC GGG GTC 
TGA CGC TCA GTG GAA CGA AAA CTC ACG TTA AGG GAT TTT GGT CAT GAG ATT ATC AAA AAG GAT CTT CAC CTA GAT CCT TTT AAA TTA AAA ATG AAG TTT 
TAA ATC AAT CTA AAG TAT ATA TGA GTA AAC TTG GTC TGA CAG CTC GAG TCC CGT CAA GTC AGC GTA ATG CTC TGC CAG TGT TAC AAC CAA TTA ACC AAT 
TCT GAT TAG AAA AAC TCA TCG AGC ATC AAA TGA AAC TGC AAT TTA TTC ATA TCA GGA TTA TCA ATA CCA TAT TTT TGA AAA AGC CGT TTC TGT AAT GAA 
GGA GAA AAC TCA CCG AGG CAG TTC CAT AGG ATG GCA AGA TCC TGG TAT CGG TCT GCG ATT CCG ACT CGT CCA ACA TCA ATA CAA CCT ATT AAT TTC CCC 
TCG TCA AAA ATA AGG TTA TCA AGT GAG AAA TCA CCA TGA GTG ACG ACT GAA TCC GGT GAG AAT GGC AAA AGC TTA TGC ATT TCT TTC CAG ACT TGT TCA 
ACA GGC CAG CCA TTA CGC TCG TCA TCA AAA TCA CTC GCA TCA ACC AAA CCG TTA TTC ATT CGT GAT TGC GCC TGA GCG AGA CGA AAT ACG CGA TCG CTG 
TTA AAA GGA CAA TTA CAA ACA GGA ATC GAA TGC AAC CGG CGC AGG AAC ACT GCC AGC GCA TCA ACA ATA TTT TCA CCT GAA TCA GGA TAT TCT TCT AAT 
ACC TGG AAT GCT GTT TTC CCG GGG ATC GCA GTG GTG AGT AAC CAT GCA TCA TCA GGA GTA CGG ATA AAA TGC TTG ATG GTC GGA AGA GGC ATA AAT TCC 
GTC AGC CAG TTT AGT CTG ACC ATC TCA TCT GTA ACA TCA TTG GCA ACG CTA CCT TTG CCA TGT TTC AGA AAC AAC TCT GGC GCA TCG GGC TTC CCA TAC 
AAT CGA TAG ATT GTC GCA CCT GAT TGC CCG ACA TTA TCG CGA GCC CAT TTA TAC CCA TAT AAA TCA GCA TCC ATG TTG GAA TTT AAT CGC GGC CTG GAG 
CAA GAC GTT TCC CGT TGA ATA TGG CTC ATA ACA CCC CTT GTA TTA CTG TTT ATG TAA GCA GAC AGT TTT ATT GTT CAT GAT GAT ATA TTT TTA TCT TGT 
GCA ATG TAA CAT CAG AGA TTT TGA GAC ACA ACG TGG CTT TGT TGA ATA AAT CGA ACT TTT GCT GAG TTG AAG GAT CAG CTC GAG TGC CAC CTG ACG TCT 
AAG AAA CCA TTA TTA TCA TGA CAT TAA CCT ATA AAA ATA GGC GTA TCA CGA GGC AGA ATT TCA GAT AAA AAA AAT CCT TAG CTT TCG CTA AGG ATG ATT 
TCT GGA ATT CGC GGC CGC TTC TAG AG

BioBricks from iGEM Parts Registry Distributions

[Test use] J04450 Part-only sequence (1069bp)

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CAA TAC GCA AAC CGC CTC TCC CCG CGC GTT GGC CGA TTC ATT AAT GCA GCT GGC ACG ACA GGT TTC CCG ACT GGA AAG CGG GCA GTG AGC GCA ACG CAA 
TTA ATG TGA GTT AGC TCA CTC ATT AGG CAC CCC AGG CTT TAC ACT TTA TGC TTC CGG CTC GTA TGT TGT GTG GAA TTG TGA GCG GAT AAC AAT TTC ACA 
CAT ACT AGA GAA AGA GGA GAA ATA CTA GAT GGC TTC CTC CGA AGA CGT TAT CAA AGA GTT CAT GCG TTT CAA AGT TCG TAT GGA AGG TTC CGT TAA CGG 
TCA CGA GTT CGA AAT CGA AGG TGA AGG TGA AGG TCG TCC GTA CGA AGG TAC CCA GAC CGC TAA ACT GAA AGT TAC CAA AGG TGG TCC GCT GCC GTT CGC 
TTG GGA CAT CCT GTC CCC GCA GTT CCA GTA CGG TTC CAA AGC TTA CGT TAA ACA CCC GGC TGA CAT CCC GGA CTA CCT GAA ACT GTC CTT CCC GGA AGG 
TTT CAA ATG GGA ACG TGT TAT GAA CTT CGA AGA CGG TGG TGT TGT TAC CGT TAC CCA GGA CTC CTC CCT GCA AGA CGG TGA GTT CAT CTA CAA AGT TAA 
ACT GCG TGG TAC CAA CTT CCC GTC CGA CGG TCC GGT TAT GCA GAA AAA AAC CAT GGG TTG GGA AGC TTC CAC CGA ACG TAT GTA CCC GGA AGA CGG TGC 
TCT GAA AGG TGA AAT CAA AAT GCG TCT GAA ACT GAA AGA CGG TGG TCA CTA CGA CGC TGA AGT TAA AAC CAC CTA CAT GGC TAA AAA ACC GGT TCA GCT 
GCC GGG TGC TTA CAA AAC CGA CAT CAA ACT GGA CAT CAC CTC CCA CAA CGA AGA CTA CAC CAT CGT TGA ACA GTA CGA ACG TGC TGA AGG TCG TCA CTC 
CAC CGG TGC TTA ATA ACG CTG ATA GTG CTA GTG TAG ATC GCT ACT AGA GCC AGG CAT CAA ATA AAA CGA AAG GCT CAG TCG AAA GAC TGG GCC TTT CGT 
TTT ATC TGT TGT TTG TCG GTG AAC GCT CTC TAC TAG AGT CAC ACT GGC TCA CCT TCG GGT GGG CCT TTC TGC GTT TAT A

[Used] K314103 Part-only sequence (1638bp)

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ACG CGC CCT GTA GCG GCG CAT TAA GCG CGG CGG GTG TGG TGG TTA CGC GCA GCG TGA CCG CTA CAC TTG CCA GCG CCC TAG CGC CCG CTC CTT TCG CTT 
TCT TCC CTT CCT TTC TCG CCA CGT TCG CCG GCT TTC CCC GTC AAG CTC TAA ATC GGG GGC TCC CTT TAG GGT TCC GAT TTA GTG CTT TAC GGC ACC TCG 
ACC CCA AAA AAC TTG ATT AGG GTG ATG GTT CAC GTA GTG GGC CAT CGC CCT GAT AGA CGG TTT TTC GCC CTT TGA CGT TGG AGT CCA CGT TCT TTA ATA 
GTG GAC TCT TGT TCC AAA CTG GAA CAA CAC TCA ACC CTA TCT CGG TCT ATT CTT TTG ATT TAT AAG GGA TTT TGC CGA TTT CGG CCT ATT GGT TAA AAA 
ATG AGC TGA TTT AAC AAA AAT TTA ACG CGA ATT TTA ACA AAA TAT TAA CGT TTA CAA TTT ACT AGA GGT GGT GAA TGT GAA ACC AGT AAC GTT ATA CGA 
TGT CGC AGA GTA TGC CGG TGT CTC TTA TCA GAC CGT TTC CCG CGT GGT GAA CCA GGC CAG CCA CGT TTC TGC GAA AAC GCG GGA AAA AGT GGA AGC GGC 
GAT GGC GGA GCT GAA TTA CAT TCC CAA CCG CGT GGC ACA ACA ACT GGC GGG CAA ACA GTC GTT GCT GAT TGG CGT TGC CAC CTC CAG TCT GGC CCT GCA 
CGC GCC GTC GCA AAT TGT CGC GGC GAT TAA ATC TCG CGC CGA TCA ACT GGG TGC CAG CGT GGT GGT GTC GAT GGT AGA ACG AAG CGG CGT CGA AGC CTG 
TAA AGC GGC GGT GCA CAA TCT TCT CGC GCA ACG CGT CAG TGG GCT GAT CAT TAA CTA TCC GCT GGA TGA CCA GGA TGC CAT TGC TGT GGA AGC TGC CTG 
CAC TAA TGT TCC GGC GTT ATT TCT TGA TGT CTC TGA CCA GAC ACC CAT CAA CAG TAT TAT TTT CTC CCA TGA AGA CGG TAC GCG ACT GGG CGT GGA GCA 
TCT GGT CGC ATT GGG TCA CCA GCA AAT CGC GCT GTT AGC GGG CCC ATT AAG TTC TGT CTC GGC GCG TCT GCG TCT GGC TGG CTG GCA TAA ATA TCT CAC 
TCG CAA TCA AAT TCA GCC GAT AGC GGA ACG GGA AGG CGA CTG GAG TGC CAT GTC CGG TTT TCA ACA AAC CAT GCA AAT GCT GAA TGA GGG CAT CGT TCC 
CAC TGC GAT GCT GGT TGC CAA CGA TCA GAT GGC GCT GGG CGC AAT GCG CGC CAT TAC CGA GTC CGG GCT GCG CGT TGG TGC GGA CAT CTC GGT AGT GGG 
ATA CGA CGA TAC CGA AGA CAG CTC ATG TTA TAT CCC GCC GTT AAC CAC CAT CAA ACA GGA TTT TCG CCT GCT GGG GCA AAC CAG CGT GGA CCG CTT GCT 
GCA ACT CTC TCA GGG CCA GGC GGT GAA GGG CAA TCA GCT GTT GCC CGT CTC ACT GGT GAA AAG AAA AAC CAC CCT GGC GCC CAA TAC GCA AAC CGC CTC 
TCC CCG CGC GTT GGC CGA TTC ATT AAT GCA GCT GGC ACG ACA GGT TTC CCG ACT GGA AAG CGG GCA GTG ATA CTA GAG AAT TGT GAG CGG ATA ACA ATT 
GAC ATT GTG AGC GGA TAA CAA GAT ACT GAG CAC ATA CTA GAG AAA GAG GAG AAA

[Test use] R0010 Part-only sequence (200bp)

...

1
100
199

CAA TAC GCA AAC CGC CTC TCC CCG CGC GTT GGC CGA TTC ATT AAT GCA GCT GGC ACG ACA GGT TTC CCG ACT GGA AAG CGG GCA GTG AGC GCA ACG CAA 
TTA ATG TGA GTT AGC TCA CTC ATT AGG CAC CCC AGG CTT TAC ACT TTA TGC TTC CGG CTC GTA TGT TGT GTG GAA TTG TGA GCG GAT AAC AAT TTC ACA 
CA

[Not used] R0011 Part-only sequence (55bp)

...

1	AAT TGT GAG CGG ATA ACA ATT GAC ATT GTG AGC GGA TAA CAA GAT ACT GAG CAC A

[Not used] K206000 Part-only sequence (130bp)

...

1 100	ACA TTG ATT ATT TGC ACG GCG TCA CAC TTT GCT ATG CCA TAG CAA GAT AGT CCA TAA GAT TAG CGG ATC CTA CCT GAC GCT TTT TAT CGC AAC TCT CTA CTG TTT CTC CAT ACC GTT TTT TTG GGC TAG C

[Test use] B0034 Part-only sequence (12bp)

...

1	AAA GAG GAG AAA

[Test use] K518012 Part-only sequence (828bp)

...

TCA CAC AGG AAA GTA CTA GAT GGC TTC CTC CGA AGA CGT TAT CAA AGA GTT CAT GCG TTT CAA AGT TCG TAT GGA AGG TTC CGT TAA CGG TCA CGA GTT 
CGA AAT CGA AGG TGA AGG TGA AGG TCG TCC GTA CGA AGG TAC CCA GAC CGC TAA ACT GAA AGT TAC CAA AGG TGG TCC GCT GCC GTT CGC TTG GGA CAT 
CCT GTC CCC GCA GTT CCA GTA CGG TTC CAA AGC TTA CGT TAA ACA CCC GGC TGA CAT CCC GGA CTA CCT GAA ACT GTC CTT CCC GGA AGG TTT CAA ATG 
GGA ACG TGT TAT GAA CTT CGA AGA CGG TGG TGT TGT TAC CGT TAC CCA GGA CTC CTC CCT GCA AGA CGG TGA GTT CAT CTA CAA AGT TAA ACT GCG TGG 
TAC CAA CTT CCC GTC CGA CGG TCC GGT TAT GCA GAA AAA AAC CAT GGG TTG GGA AGC TTC CAC CGA ACG TAT GTA CCC GGA AGA CGG TGC TCT GAA AGG 
TGA AAT CAA AAT GCG TCT GAA ACT GAA AGA CGG TGG TCA CTA CGA CGC TGA AGT TAA AAC CAC CTA CAT GGC TAA AAA ACC GGT TCA GCT GCC GGG TGC 
TTA CAA AAC CGA CAT CAA ACT GGA CAT CAC CTC CCA CAA CGA AGA CTA CAC CAT CGT TGA ACA GTA CGA ACG TGC TGA AGG TCG TCA CTC CAC CGG TGC 
TTA ATA ACG CTG ATA GTG CTA GTG TAG ATC GCT ACT AGA GTC ACA CTG GCT CAC CTT CGG GTG GGC CTT TCT GCG TTT ATA TAC TAG AGA GAG AAT ATA 
AAA AGC CAG ATT ATT AAT CCG GCT TTT TTA TTA TTT

[Not used] B0012 Part-only sequence (41bp)

...

1	TCA CAC TGG CTC ACC TTC GGG TGG GCC TTT CTG CGT TTA TA

Full table

No.	ID	Name / Function	Source	Gene Size / Sequence	Initial Plasmid / Vector	Comments
1	pAzoR / BBa_K1336000	FMN-dependent NADH-azoreductase 1	Pseudomonas putida	612 bp	Expression vector pET-21a (+) (ampicillin resistant (ampR)), initially cloned between NdeI and BamHI.	Plasmid provided by Lisbon; with Paper.
2	p1B6 (AzoR 1B6) / BBa_K1336001	Mutant: Heat-stable; FMN-dependent NADH-azoreductase 1	Pseudomonas putida	612 bp	Expression vector pET-21a (+) (ampR), initially cloned between NdeI and BamHI.	Plasmid provided by Lisbon; with Paper.
3	pCotA / BBa_K1336002	Spore Coat Protein Laccase	Bacillus subtilis	1542 bp	Expression vector pET-21a (+) (ampR), initially cloned between *NheI* and BamHI.	Plasmid provided by Lisbon; with Paper.
4	pBsDyP / BBa_K1336003	Dye Decolourising Peroxidase BSU38260	Bacillus subtilis	1251 bp	Expression vector pET-21a (+) (ampR), initially cloned between NdeI and BamHI.	Plasmid provided by Lisbon; with Paper.
5	pPpDyP / BBa_K1336004	Dye Decolourising Peroxidase PP_3248	Pseudomonas putida	864 bp	Expression vector pET-21a (+) (ampR), initially cloned between NdeI and BamHI.	Plasmid provided by Lisbon; with Paper.
6	LiP / BBa_K500000	Lignin Peroxidase	Phanerochaete chrysosporium (White-Rot Fungi)	1116 bp	[To be cloned directly into expression vector, pSB1C3, between EcoRI and PstI.]	Synthesised (for free) by Gene Oracle. Sequence from Paper. Optimised for use in E. coli.
7	CueO / BBa_K729006	Laccase	Escherichia coli	1612 bp	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR).	A device consisting of: a constituitive promoter (J23119), a strong RBS (B0034), and laccase for polyethylene degradation (K729002).
8	ispB / BBa_K1336005	Octaprenyl Diphosphate Synthase	Escherichia coli, strain K-12	972 bp	[To be cloned directly into expression vector, pSB1C3, between EcoRI and PstI.]	Using the antisense/reverse complement gene as a method of gene knockdown (i.e. as RNAi); in collaboration with the Edinburgh iGEM 2014 Team.
9	BBa_J04450	RFP Coding Device	Spring 2014 BioBrick Distribution. Plate 4, Well 4B. [Check! DT?].	1069 bp, Genomic Sequence[Add sequence! Made from combined BioBricks?]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR).	LacI-, and CAP-, sensitive; can fail if system contains LacI or CAP protein! RFP Coding Device contains: LacI (R0010), strong RBS (B0034), mRFP1 (E1010), and double terminator (B0015 = B0010+B0012).
10	BBa_K314103	Lac induced expression cassette	Spring 2014 BioBrick Distribution. Plate 1, Well 4D. [Check! DT?].	1638 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map.	Lactose (IPTG) inducible protein expression insert includes f1 origin (K314110), a Lac I generator (K314111), a lactose inducible promoter (R0011), and the Elowitz standard RBS (B0034).
11	BBa_R0010	Promoter (LacI regulated)	Spring 2014 BioBrick Distribution. Plate 3, Well 4G. [Check! DT?].	200 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map.	This part is an inverting regulator sensitive to LacI and CAP. In the absence of LacI and CAP proteins, this part promotes transcription; in their presence, the part inhibits transcription. LacI can be inhibited by IPTG.
12	BBa_R0011	Promoter (LacI regulated, lambda pL hybrid)	Spring 2014 BioBrick Distribution. Plate 2, Well 6D (Inconsistent sequencing!). [Check! DT? / Maybe use Spring 2013 Distribution, Plate 5, Well 6G.].	55 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map.	Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1.
13	BBa_K206000	pBAD Strong Promoter	Spring 2014 BioBrick Distribution. Plate 3, Well 14A. [Check! DT?].	130 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map	pBAD is an E. coli promoter that is induced by L-arabinose. In the absence of arabinose, the repressor protein AraC (BBa_I13458) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription; in its presence, transcription is permitted.
14	BBa_B0034	RBS	Spring 2014 BioBrick Distribution. Plate 4, Well 1N. [Check! DT?].	12 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1A2, i.e. ampicillin resistant (ampR). Plasmid / Vector Map	RBS based on Elowitz (1999) repressilator.
15	BBa_K518012	RBS + RFP + double Terminator	Spring 2014 BioBrick Distribution. Plate 1, Well 18C. [Check! DT?].	828 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map	This Coding Device contains: RBS.3 (medium) (B0032), mRFP1 (E1010), and double terminator (B0014 = B0012+B0011).
16	[CHECK: BAD PART !] BBa_B0012 (2)	Transcription Terminator for E. coli RNA polymerase	Spring 2014 BioBrick Distribution. Plate 2, Well 2B. [Check! DT?].	41 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map	TE from coliphage T7. This is a bad terminator (Experience: Fails). It is a promoter in the reverse direction.
#	ID	Name / Function	Source	Gene Size / Sequence	Initial Plasmid / Vector	Comments