Team:UC Davis/Electrochemistry

Having settled on NAD⁺ dependent Aldehyde Dehydrogenases as our method of differentiating between aldehydes, we needed to develop an efficient electrode system to detect enzyme activity via NADH. We acquired, selected, and optimized an electrode setup for the detection of NADH at low concentrations in a complex solution. Additionally, we demonstrated the ability of the electrode setup to detect enzyme generated NADH over time, and thereby functionally deconvolute aldehyde profiles within a sample.

Our Electrode system needed to:

• Sensitive: have a low limit of detection for NADH
• Reactive: Detect NADH with high linear range
• Selective: Be robust to any possible solution components
• Affordable: Cost accessible to the average consumer
• Be efficient: Use a low sample volume
• Be compatible: Be compatible with our, as well as other potentiostats
• Be portable

We tested three base electrode types,and five different working electrode modifications in order to achieve the requisite sensitivity for our system.

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