Team:UC Davis/Electrochemistry

The Electrochemistry team acquired, selected, and optimized an electrode setup for the detection of NADH at low concentrations in a complex solution. Additionally, the team demonstrated the ability of the electrode setup to detect enzyme generated NADH over time, and thereby functionally deconvolute aldehyde profiles within a sample.

Having settled on NAD⁺ dependent Aldehyde Dehydrogenases as our method of differentiating between aldehydes, we needed to develop an efficient electrode system to detect enzyme activity via NADH.

Our Electrode system needed to:

• Detect NADH at low LOD (Sensitive)
• Detect NADH with high linear range (Reactive)
• Be robust to any possible solution components (Selective)
• Be cheap (low cost)
• Use a low sample volume (be efficient)
• Be compatible and interfacaeble with potentiostat
• Be Portable

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