Interlab study

Our team has contributed to the Interlab study not only by measuring some of the proposed constructions but also by sharing the results of similar constructions built for our own project. To date, you can check in this page the results we got for the device BBa_I20260. More data on our Biobricks is coming soon!

Fluorescence emission by Biobrick part BBa_I20260

We used the famous E. coli strain DH5α as a host for the expression of this construction. Briefly, the strain carrying the Biobrick part in the pSB3K3 vector was striked on LB medium supplemented with kanamycin. A liquid culture was set up in the same medium and grew in agitation (250 rpm) at 37C until OD600 reached a value around 0,2. The fluorescence of this culture was measured and the results are shown in Figure 1. We used the wild-type DH5α E. coli strain as a control, and performed all the experiments with five independent replica.

Figure 1. Fluorescence emission of DH5α E. coli strain containing Biobrick part BBa_I20260 vs the control non-transformed strain. The average and the standard deviation of five independent biological replicates is shown. Fluorescence intensity (FI) was normalized by the optical density of the cultures measured at 600 nm (OD600).

You can see a more detailed explanation of the protocols we used in our Interlab Study Worksheet!