Team:Caltech/Notebook

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Notebook

Apparently there's a calendar feature to take care of this too???

Week One

Tuesday, 6/17/14

  • Get acquainted with summer logistics and lab basics
  • Clean up lab space, stock lab supplies
We got absolutely no results today!!!

Wednesday, 6/18/14

  • Continue to discuss project and clean up lab space
  • Designed PCR primers for combinatorial promoters subproject
We got absolutely no results today!!!

Thursday, 6/19/14

  • Formulated plasmid design for the response regulation subproject
  • PCR plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly
  • Gel electrophoresis for confirmation of PCR products' length
With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis

Friday, 6/20/14

  • Purification of PCR products created yesterday
  • Gibson assembly of purified products
  • Transformation of Gibson-assembled plasmids into JM109 E. coli
  • Meeting with Prof. Murray to discuss give an update on the project
Colonies were found and picked after transformation and incubation.

Week Two

Monday, 6/23/14

  • Discussion of fallout from Friday's meeting, paper reading
  • Colony PCR and Minipreps of liquid cultures of colonies picked over the weekend
  • TXTL workshop starts today

Tuesday, 6/24/14

Wednesday, 6/25/14

Thursday, 6/26/14

Colonies were found and picked after transformation and incubation.