iGEM Team Aachen BioBricks
[http://parts.igem.org/Part:BBa_K1319000 K1319000]
RFC [25] Version of E0020
This part is an RFC [25]-compatible version of BBa_E0030. The start and stop codons have been removed to make it RFC [25]-compatible and the part is flanked by the RFC [25] prefix- and suffix-sequences.
The coding sequence encodes EYFP (enhanced yellow fluorescent protein) which is derived from A. victoria GFP. The excitation is 512 nm and the emission is 534 nm.
[http://parts.igem.org/Part:BBa_K1319001 K1319001]
RFC [25] - compatible dark quencher based on K1319000 (E0030)
This part is an RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP).
Two mutations were introduced that eliminated fluorescence:
[http://parts.igem.org/Part:BBa_K1319002 K1319002]
RFC [25] - compatible dark quencher based on K1319000 (E0030)
This part is an RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP).
Three mutations were introduced that eliminated fluorescence:
[http://parts.igem.org/Part:BBa_K1319003 K1319003]
human galectin-3, codon optimized for E. coli
The trunkated galectin-3 is a 26 kDa protein that binds certain LPS patterns.
[http://parts.igem.org/Part:BBa_K1319004 K1319004]
TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli
This part is a TEV protease in RFC25 that was codon-optimized for expression in E. coli. The part contains the S219V anti-self cleavage mutation.
The TEV Protease (also known as Tobaco Edge Virus nuclear inclusion a endopeptidase) is a highly sequence specific cysteine protease from the Tobacco Edge Virus (TEV). The protease is highly sequence specific. The consensus sequence for the cut is ENLYFQ\S with \ denoting the cleaved peptide bond. This sequence can be found in the part [http://parts.igem.org/Part:BBa_K1319016 K1319016].
ENLYFQ\S is the optimal cleavage site with the highest activity but the protease is also active to a greater or lesser extent on a range of substrates. The highest cleavage is of sequences closest to the consensus EXLYΦQ\φ where X is any residue, Φ is any large or medium hydrophobic amino acid and φ is any small hydrophobic amino acid.
The TEV protease is commonly used as a biochemical tool to cleave affinity tags from purified proteins like [http://parts.igem.org/Part:BBa_K1319007 His-Tags]. The high specifity makes the protease relatively non-toxic in vitro and in vivo. The molecular weight of the TEV protease is 27 kDa.
[http://parts.igem.org/Part:BBa_K1319008 K1319008]
IPTG-induced and T7-driven expression of TEV protease
This protein generator produces [http://parts.igem.org/Part:BBa_K1319004 TEV protease] when induced with IPTG in a DE3 strain or if combined with a T7 RNA-Polymerase generator.
[http://parts.igem.org/Part:BBa_K1319010 K1319010]
Constitutive expression of K1319000
This part expresses K1319000 behind a J23101 constitutive promotor.
[http://parts.igem.org/Part:BBa_K1319011 K1319011]
Constitutive expression of K1319001
This part expresses K1319001 behind a J23101 constitutive promotor.
[http://parts.igem.org/Part:BBa_K1319012 K1319012]
Constitutive expression of K1319002
This part expresses K1319002 behind a J23101 constitutive promotor.
[http://parts.igem.org/Part:BBa_K1319013 K1319013]
Constitutive expression of GFP-REACh1 fusion protein
This part expresses a E0040.K1319001 fusion protein (GFP-REACh1) behind a J23101 promotor.
[http://parts.igem.org/Part:BBa_K1319014 K1319014]
Constitutive expression of GFP-REACh2 fusion protein
This part expresses a E0040.K1319002 fusion protein (GFP-REACh1) behind a J23101 promotor.
[http://parts.igem.org/Part:BBa_K1319015 K1319015]
Constitutive expression of GFP-EYFP fusion protein
This part expresses a E0040.K1319000 fusion protein (GFP-EYFP) behind a J23101 promotor.
[http://parts.igem.org/Part:BBa_K1319016 K1319016]
TEV protease cleavage site
This sequence codes for a [http://parts.igem.org/Part:BBa_K1319004 TEV protease] cleavage site.
ENLYFQ\S is the optimal cleavage site with the highest activity but the protease is also active to a greater or lesser extent on a range of substrates. The highest cleavage is of sequences closest to the consensus EXLYΦQ\φ where X is any residue, Φ is any large or medium hydrophobic amino acid and φ is any small hydrophobic amino acid.
[http://parts.igem.org/Part:BBa_K1319017 K1319017]
LasI induced iLOV
This device produces iLOV (K660004) in response to a quorum sensing input.
[http://parts.igem.org/Part:BBa_K1319020 K1319020]
Translational unit of mRFP-galectin3-His
This part is a translational unit of a mRFP-galectin-3-His (B0032.E1010.K1319003.K1319016.B0015).
[http://parts.igem.org/Part:BBa_K1319042 K1319042]
IPTG inducible iLOV
This part can be used for IPTG-induced expression of K660004 (iLOV).
- Part Name
- Part type
- Creator
- Sequence
- Short Description (60 characters on what the DNA does)
- Long Description (Longer description of what the DNA does)
- Design considerations
<groupparts>iGEM14 Aachen</groupparts>
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