How our system works:
[Diagram with Biobrick Code]
Data For Our Favorite New Parts:
- SigmaX, BBa_K1379004 : gene sequence of competence protein comX which produces σx factor. σx factor induces promoter containing combox sequence (PcelA and Phelicase).
- PcelA promoter, BBa_K1379000 : a σx-inducible promoter (containing combox sequence) which initiates the transcription of competence CelA protein.
- Phelicase promoter, BBa_K1379001 : a σx-inducible promoter (containing combox sequence) which initiates the transcription of helicase protein.
Data for Pre-existing Parts:
- Strong promoter, strong RBS combination, BBa_K880005 (Team Michigan iGEM, 2012) : combination of strong constitutive promoter ( BBa_J23100) and strong RBS ( BBa_B0034 ) was used as a backbone for a very high level of expression of ComX protein (σx factor).
- GFP generator, BBa_E0240 (Antiquity Boston University iGEM, 2004) : green fluorescent protein reporter ( BBa_E0040 ) with RBS ( BBa_B0032) and double terminator ( BBa_B0015) was used as a reporter for characterization of PcelA and Phelicase promoters.
We Also Built and Characterized the Following Parts
- SigmaX Generator, BBa_K1379006 : comX gene sequence was assembled with constitutive promoter, RBS and double terminator so ComX protein is produced constitutively.
- PcelA-GFP Generator, BBa_K1379002 : σx-inducible PcelA promoter was assembled with GFP generator.
- Phelicase-GFP Generator, BBa_K1379003 : σx-inducible Phelicase promoter was assembled with GFP generator.
- SigmaX Generator, PcelA-GFP Generator, BBa_K1379005 : σx-generator was assembled with PcelA-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.
- SigmaX Generator, Phelicase-GFP Generator, BBa_K1379007 : σx-generator was assembled with Phelicase-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.
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