1. • Adjust total volume of all PCR tubes to 100 μL
   • Add 500 μL Binding Buffer to each tube
   • Mix well
2. • Insert one High Pure filter tube into one collection tube
   • Transfer solution from 1 into the filter
   • Centrifuge 60 seconds at maximum speed
3. • Disconnect filter tube and discard flow-through
   • Reconnectn tubes
4. • Add 500 μL wash buffer to upper reservoir
   • Centrifuge 60 seconds at maximum speed
5. • Discard the flow-through
   • Recombine tubes
   • Add 200 μL wash buffer
   • Centrifuge for 60 seconds at maximum speed
6. • Discard flow-through and collection tube
   • Reconnect filter tube to a clean 1.5 mL centrifuge tube
7. • Add 100 μL elution buffer
   • Centrifuge for 1 minute at maximum speed
8. • The purified PCR product is now in the microcentrifuge tube
   • Store at 4 DEGC for short term or at -20 DEGC for later use

Thank you to Roche (High Pure PCR Product Purification Kit) for this protocol.

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