Team:UCL/Science/Sequences

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Goodbye Azodye UCL iGEM 2014

Gene and BioBrick Part Sequences

Laboratory Team

Summary

For ease of reference, the sequences for (1) our BioBrick Gene Designs, (2) Plasmid / Vector Backbones, and (3) BioBricks that we have used from that iGEM Registry Distribution, have been provided below. Clicking on the Gene/Plasmid/BioBrick headers will take you to pages describing the part in more detail and the source of the sequences.

Note: format is a little horrendous, will change to all CAPS, and at a set width for all later.

BioBrick Designs for Our Genes

[New] AzoR Part-only sequence (597/612bp) [Link, see DT]

Sequence taken from Lisbon.
ATGAAACTGTTGCACATCGATTCGAGCATCCTGGGCGACAATTCCGCCTCGCGTCAGCTGAGCCGTGAAG TGGTCGAAGCCTGGAAGGCTGCAGACCCAAGCGTTGAAGTGGTTTATCGCGACCTGGCTGCCGATGCCAT CGCCCACTTCTCCGCTGCCACCCTGGTCGCTGCAGGCACCCCTGAAGACGTACGCGACGCGGCCCAGGCT TTCGAAGCCAAGCTGAGCGCCGAGACGCTCGAAGAGTTCCTGGCTGCCGATGCCGTGGTGATTGGTGCGC CCATGTACAACTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGAC CTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCG GGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCA TCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGC GATCGATGCTGCACAGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGA

[New] AzoR 1B6 (heat-stable AzoR mutant) Part-only sequence (597/612bp) [Link, see DT]

Sequence given from Lisbon group directly.
ATGAAACTGTTGCACATCGATTCGAGCATCCTGGGCGACAATTCCGCCTCGCGCCAGCTGAGCC GTGAAGTGGTCGAAGCCTGGAAGGCTGCAGACCCAAGCGTGGAAGTGGTTTATCGCGACCTGGCTGCCGA TCCCATCGCCCACTTCTCCGCTGCCACCCTGGTCGCTGCAGGCACCCCTGAAGACGTACGCGACGCGGCC CAGGCTTTCGAAGCCAAGCTGAGCGCCGAGACGCTCGAAGAGTTCCTGGCTGCCGATGGCGTGGTGATTG GTGCGCCCATGTACAACTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGG CAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGAGCGGCGACAAGAAAGTGGTGCTGGTTTCC ACTGCGGGTGGCCAGCATGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGCGTTCCTGG GCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGACTATGGCCCGGAGCAGCGCAG CCAGGCGATAGATGCTGCACGGGCGCAGATTGCCAGCGAGCTGTTTGCGGCTGCCTGA

[Proposed] CotA Part-only sequence (1733/1539bp) [Link, see DT]

Sequence given from Lisbon group directly.
atgacacttgaaaaatttgtggatgctctcccaatcccagatacactaaa gccagtacagcaatcaaaagaaaaaacatactacgaagtcaccatggagg aatgcactcatcagctccatcgcgatctccctccaacccgcctgtggggc tacaacggcttatttccgggaccgaccattgaggttaaaagaaatgaaaa cgtatatgtaaaatggatgaataaccttccttccacgcatttccttccga ttgatcacaccattcatcacagtgacagccagcatgaagagcccgaggta aagactgttgttcatttacacggcggcgtcacgccagatgatagtgacgg gtatccggaggcttggttttccaaagactttgaacaaacaggaccttatt tcaaaagagaggtttatcattatccaaaccagcagcgcggggctatattg tggtatcacgatcacgccatggcgctcaccaggctaaatgtctatgccgg acttgtcggtgcatatatcattcatgacccaaaggaaaaacgcttaaaac tgccttcagacgaatacgatgtgccgcttcttatcacagaccgcacgatc aatgaggatggttctttgttttatccgagcgcaccggaaaacccttctcc gtcactgcctaatccttcaatcgttccggctttttgcggagaaaccatac tcgtcaacgggaaggtatggccatacttggaagtcgagccaaggaaatac cgattccgtgtcatcaacgcctccaatacaagaacctataacctgtcact cgataatggcggagattttattcagattggttcagatggagggctcctgc cgcgatctgttaaactgaattctttcagccttgcgcctgctgaacgttac gatatcatcattgacttcacagcatatgaaggagaatcgatcattttggc aaacagcgcgggctgcggcggtgacgtcaatcctgaaacagatgcgaata tcatgcaattcagagtcacaaaaccattggcacaaaaagacgaaagcaga aagccgaagtacctcgcctcatacccttcggtacagcatgaaagaataca aaacatcagaacgttaaaactggcaggcacccaggacgaatacggcagac ccgtccttctgcttaataacaaacgctggcacgatcccgtcacagaaaca ccaaaagtcggcacaactgaaatatggtccattatcaacccgacacgcgg aacacatccgatccacctgcatctagtctccttccgtgtattagaccggc ggccgtttgatatcgcccgttatcaagaaagcggggaattgtcctatacc ggtccggctgtcccgccgccgccaagtgaaaagggctggaaagacaccat tcaagcgcatgcaggtgaagtcctgagaatcgcggcgacattcggtccgt acagcggacgatacgtatggcattgccatattctagagcatgaagactat gacatgatgagaccgatggatataactgatccccataaataa

[New] BsDyP Part-only sequence (1251bp) [Link, see DT]

Sequence given from Lisbon group directly.
atgagcgatgaacagaaaaagccagaacaaattcacagacgggacattttaaaatgggga gcgatggcgggggcagccgttgcgatcggtgccagcggtctcggcggtctcgctccgctt gttcagactgcggctaagccatcgaaaaaggatgaaaaagaggaggagcagattgttccg ttttacggaaagcatcaggccggaatcacaactgcccatcagacgtatgtctattttgct gcactggatgtaacagcaaaagacaaaagcgacatcattacgttatttagaaactggaca agtctcacacagatgctgacgtctggcaagaaaatgtcagcggagcagagaaatcagtat ctgccgccgcaggatacaggtgagtctgctgatttatccccttccaacttaacggtcaca ttcggattcgggcctggcttttttgaaaaagacggaaaggaccgcttcggactgaaaagc aaaaaaccgaagcacctagccgctcttccagcgatgcccaatgacaacctggatgaaaag cagggaggcggggacatctgcattcaagtgtgtgcagacgacgagcaagtggcgtttcac gcgctgcgtaacctgctaaatcaagcggtcggcacttgtgaggtccgttttgtgaacaaa ggctttttaagcggtggaaaaaatggtgaaacaccgcgcaacctcttcgggtttaaagat ggaacaggcaaccagagcacgaaggatgacacgttgatgaactcgatcgtgtggattcag tctggtgagcctgactggatgacgggcggcacctatatggcttttcggaaaatcaaaatg ttccttgaggtatgggaccgctcttccctcaaggaccaagaggataccttcggccgcaga aaaagctcgggggcgccttttgggcagaaaaaggaaacagatcccgtgaagctgaatcaa attccgtctaattcacacgtcagtctagcgaaatctactgggaaacaaattttgcgaaga gctttctcttacacagaaggacttgatccgaaaaccggctatatggatgcaggtctcctg tttatcagttttcaaaaaaatcccgacaatcagtttatcccgatgctgaaggctctttca gcaaaggatgcgttaaacgaatatacacaaacaatcggttctgctttatacgcatgccca ggcggctgcaaaaaaggagaatatattgcccagcgtttgctggaatcataa

[Proposed] PpDyP Part-only sequence (861/864bp) [Link, see DT]

Sequence taken from Lisbon.
ATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCGGCCCACGCCCGTCACCTGTTCTTCACCCTGC AGTCGCCCGAGGCGTTGCCTGCCGCGCTGGATGCCTTGCTGCCGCAGGTCGATGGCAAACAGTTGCTGCT GGGCGTAGGCGCCCCCTTGGCCAAGGCCCTGGGCCGGGAAATACCAGGCCTGCGCCCGTTCCCGCTACTG GATGCCGCGGTTGAGAACCCCAGCACCCAACACGCCCTGTGGCTGTGGCTGCGTGGCGACGAACGTGGCG ACCTGCTGCTGCGCACCCAGGCCCTGGAGCAGGCGCTGGCACCCGCCCTGAGCCTGGCCGACAGCGTTGA TGGCTTCCTGCACCGGGGCGGCCATGACCTTACCGGTTATGAAGACGGCACCGAAAACCCGACAGACGAA GAGGCGGTGCAAGCGGCCATTGCCGCAGATGGCTCCAGCTTTGCCGCGTTCCAGCTGTGGAAGCACGACC TGCAATACTTCAAGTCGCTGCCCCAGGCAGACCAGGACAACATCATCGGCCGTCGCTTGAGCGATAACGA AGAACTCGACGACGCCCCCGCGTCCGCGCACGTCAAGCGCACCGCCCAGGAAAGCTTTGAACCCGAGGCG TTCATGGTCCGTCGCTCGGTATCCTGGGCCGACCAACGCGGCGCCGGCCTGGCTTTCGTCGCCCTGGGCA AGAGTTTCGAGGCATTCGAGGTGCAGCTGCGGCGCATGAGTGGCCTGGAAGACGGCATCATCGACGGGCT GTACCGCTTCAGCCGCCCGCTGACGGGGGGCTACTACTGGTGCCCGCCAATGAGCGAGACGGGTGTCGAT CTAAGCCCCTTGCTGCGGGCCTGA

[Improvement] LiP Part-only sequence (1116bp) [Link, see DT]

Sequence taken from the initial page; not currently showing our improvements.
atggcttttaaacaattattcgccgctatttccttggctttgtctttgtctgctgctaatgctgctgctgttattgaaaaaagagctacttgttccaacg gtaagactgttggtgatgcttgttcttgtgcttggtttgatgttttggacgacatccaacaaaacttgtttcatggtggtcaatgtggtgctgaagctca tgaatctatcagattggttttccatgactccattgctatttctccagctatggaagctcaaggtaaatttggtggaggtggtgctgatggttccattatg attttcgatgatatcgaaaccgccttccatccaaatattggtttggacgaaatcgtcaagttgcaaaagccatttgttcaaaagcacggtgttactccag gtgattttattgcttttgctggtgctgttgctttgtctaattgtccaggtgctccacaaatgaatttctttactggtagagcaccagctactcaaccagc tccagatggtttggttccagaaccatttcataccgttgaccaaatcatcaacagagttaatgacgccggtgaatttgacgaattggaattggtttggatg ttgtctgctcattctgttgctgctgttaatgatgttgatccaactgttcaaggtttgccatttgattctaccccaggtattttcgattcccaattcttcg ttgaaacccaattgagaggtactgcttttccaggttctggtggtaatcaaggtgaagttgaatctccattgccaggtgaaattagaatccaatccgatca taccattgccagagattcaagaactgcttgtgaatggcaatccttcgttaacaatcaatccaagttggttgacgacttccaattcattttcttggctttg actcaattgggtcaagatccaaatgctatgactgattgctccgatgttattccacaatctaaacctatcccaggtaacttgccattttcattttttccag ctggtaagaccatcaaggatgttgaacaagcttgtgctgaaactccatttccaactttgactactttgccaggtccagaaacttctgttcaaagaatacc accaccaccaggtgct

[Improvement] Laccase Part-only sequence (Xbp) [Link, see DT]

Sequence taken from the initial page; not currently showing our improvements.
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgcaacgtcgtgatttcttaaaatattccgtcgcgctg ggtgtggcttcggctttgccgctgtggagccgcgcagtatttgcggcagaacgcccaacgttaccgatccctgatttgctcacgaccgatgcccgtaatc gcattcagttaactattggcgcaggccagtccacctttggcgggaaaactgcaactacctggggctataacggcaatctgctggggccggcggtgaaatt acagcgcggcaaagcggtaacggttgatatctacaaccaactgacggaagagacaacgttgcactggcacgggctggaagtaccgggtgaagtcgacggc ggcccgcagggaattattccgccaggtggcaagcgctcggtgacgttgaacgttgatcaacctgccgctacctgctggttccatccgcatcagcacggca aaaccgggcgacaggtggcgatggggctggctgggctggtggtgattgaagatgacgagatcctgaaattaatgctgccaaaacagtggggtatcgatga tgttccggtgatcgttcaggataagaaatttagcgccgacgggcagattgattatcaactggatgtgatgaccgccgccgtgggctggtttggcgatacg ttgctgaccaacggtgcaatctacccgcaacacgctgccccgcgtggttggctgcgcctgcgtttgctcaatggctgtaatgcccgttcgctcaatttcg ccaccagcgacaatcgcccgctgtatgtgattgccagcgacggtggtctgctacctgaaccagtgaaggtgagcgaactgccggtgctgatgggcgagcg ttttgaagtgctggtggaggttaacgataacaaaccctttgacctggtgacgctgccggtcagccagatggggatggcgattgcgccgtttgataagcct catccggtaatgcggattcagccgattgctattagtgcctccggtgctttgccagacacattaagtagcctgcctgcgttaccttcgctggaagggctga cggtacgcaagctgcaactctctatggacccgatgctcgatatgatggggatgcagatgctaatggagaaatatggcgatcaggcgatggccgggatgga tcacagccagatgatgggccatatggggcacggcaatatgaatcatatgaaccacggcgggaagttcgatttccaccatgccaacaaaatcaacggtcag gcgtttgatatgaacaagccgatgtttgcggcggcgaaagggcaatacgaacgttgggttatctctggcgtgggcgacatgatgctgcatccgttccata tccacggcacgcagttccgtatcttgtcagaaaatggcaaaccgccagcggctcatcgcgcgggctggaaagataccgttaaggtagaaggtaatgtcag cgaagtgctggtgaagtttaatcacgatgcaccgaaagaacatgcttatatggcgcactgccatctgctggagcatgaagatacggggatgatgttaggg tttacggtataa

[New] ispB Part-only sequence (972bp) [Link, see EG]

Sequence shows the ispB gene, but we use the Reverse Complement to design primers to produce an antisense fragment for gene knockdown (i.e. as RNAi).ATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGT TAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATC AGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATG ATTGCTGTACTGGCTGCACGAGCTGTTGGCTATGAGGGAAATGCGCATGT CACCATTGCTGCCCTGATCGAGTTTATCCACACGGCGACTCTGCTACACG ACGACGTTGTGGATGAATCAGATATGCGCAGGGGTAAAGCTACCGCCAAC GCCGCATTTGGCAATGCCGCCAGCGTGCTGGTAGGCGATTTTATTTATAC CCGCGCTTTCCAGATGATGACCAGCCTCGGTTCGCTCAAAGTGCTGGAAG TCATGTCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTG ATGAACGTTAACGATCCGGACATCACTGAAGAAAACTACATGCGCGTTAT CTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGGA TTCTGGCTGGCTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGG CGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTACTCGATTACAA TGCCGATGGCGAACAGTTAGGTAAAAATGTCGGCGACGATCTGAACGAAG GTAAACCGACGCTGCCGCTGCTGCATGCGATGCATCATGGCACACCAGAA CAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCT TCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGA CGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAG GTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACAT CGCTGTTCAACGCGATCGTTAA

[New] ispB Reverse Complement Part-only sequence (972bp) [Link, see DT]

TTAACGATCGCGTTGAACAGCGATGTGCGCGAGGCCGATGAGTGCTTCTC GCCAAGGGGTGTCCGGGAGCACCTGTAACGCTGCGATGGCTTTGTCTGCT TCTTCCTCGGCACGCTGACGCGTCCATTCAAGAGATCCACAAGCGTTCAT TGCTTCCAGAACCGGTTCCAGAAGATGGCGACCGTTACCCTGTTCGATGG CGGTACGGATCATCTGTGCCTGTTCTGGTGTGCCATGATGCATCGCATGC AGCAGCGGCAGCGTCGGTTTACCTTCGTTCAGATCGTCGCCGACATTTTT ACCTAACTGTTCGCCATCGGCATTGTAATCGAGTAAATCGTCGATCAACT GGAAAGCAGTGCCGAGATAGCGCCCATAATCCTGCAGGCCTTTCTCCTCC TCCGGCGTACAGCCAGCCAGAATCCCGGAACACTGCGCGGCAGCCTCAAA CAGACGCGCGGTTTTGCTATAGATAACGCGCATGTAGTTTTCTTCAGTGA TGTCCGGATCGTTAACGTTCATCAGTTGCAGAACTTCACCTTCTGCGATG ACGTTTACGGCTTCTGACATGACTTCCAGCACTTTGAGCGAACCGAGGCT GGTCATCATCTGGAAAGCGCGGGTATAAATAAAATCGCCTACCAGCACGC TGGCGGCATTGCCAAATGCGGCGTTGGCGGTAGCTTTACCCCTGCGCATA TCTGATTCATCCACAACGTCGTCGTGTAGCAGAGTCGCCGTGTGGATAAA CTCGATCAGGGCAGCAATGGTGACATGCGCATTTCCCTCATAGCCAACAG CTCGTGCAGCCAGTACAGCAATCATCGGACGAATACGTTTACCGCCGCCG CTGACGATGTAATAGCCTAACTGATTGATCAGTTGGACGTCGGAATTAAG CTGCTCAAGGATTGCCGCATTAACACCCGCCATATCTTGCGCGGTTAACT CATTGATTTTTTCTAAATTCAT

Plasmid Backbone / Vectors

pET-21a (+) sequence (Xbp) [Link, see DT]

atccggatatagttcctcctttcagcaaaaaacccctcaagacccgtttagaggccccaaggggttatgctagttattgc tcagcggtggcagcagccaactcagcttcctttcgggctttgttagcagccggatctcagtggtggtggtggtggtgctc gagtgcggccgcaagcttgtcgacggagctcgaattcggatccgcgacccatttgctgtccaccagtcatgctagccata tgtatatctccttcttaaagttaaacaaaattatttctagaggggaattgttatccgctcacaattcccctatagtgagt cgtattaatttcgcgggatcgagatctcgatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcg gttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcgg cgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcgg tgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgagatcccggacac catcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatgtgaaac cagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccac gtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaact ggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcga ttaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcg gcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgt ggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctccc atgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccatta agttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacg ggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgc tggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcg gtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgct ggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcac tggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctg gcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtaagttagctcactcattaggcaccgg gatctcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgca cttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctt tcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactg gtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccccacgggtgcgcatgatcgtgctc ctgtcgttgaggacccggctaggctggcggggttgccttactggttagcagaatgaatcaccgatacgcgagcgaacgtg aagcgactgctgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaaagtctgg aaacgcggaagtcagcgccctgcaccattatgttccggatctgcatcgcaggatgctgctggctaccctgtggaacacct acatctgtattaacgaagcgctggcattgaccctgagtgatttttctctggtcccgccgcatccataccgccagttgttt accctcacaacgttccagtaaccgggcatgttcatcatcagtaacccgtatcgtgagcatcctctctcgtttcatcggta tcattacccccatgaacagaaatcccccttacacggaggcatcagtgaccaaacaggaaaaaaccgcccttaacatggcc cgctttatcagaagccagacattaacgcttctggagaaactcaacgagctggacgcggatgaacaggcagacatctgtga atcgcttcacgaccacgctgatgagctttaccgcagctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacaca tgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggt gttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatca gagcagattgtactgagagtgcaccatatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcagg cgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggc ggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaacc gtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcag aggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgac cctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatc tcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatcc ggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcag agcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggta tctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagc ggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggg gtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcc ttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatc agtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgat acgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaa taaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgc cgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggcatcgtggtgtcacg ctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaa aagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagca ctgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgaga atagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaag tgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaaccc actcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgc cgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatc agggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccc cgaaaagtgccacctgaaattgtaaacgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttt taaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagttt ggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccacta cgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccg atttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgc tggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcg cca

pSB1C3 Part-only sequence (2070bp)

tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggct tcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggata acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacga gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaag acacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac ggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctca gtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc taaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagat ggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaatt cattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcc catggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattc tcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcac tccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacg aaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaata tccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatc cagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagtt ggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggca gaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag

pSB1A3 Part-only sequence (2155bp)

tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggct tcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggata acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacga gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaag acacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac ggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctca gtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc taaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcct gactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagattt atcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctaga gtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccg gttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgc agtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtca ttctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattg gaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttt tactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttc ctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgca catttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaa aaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag

pSB1K3 Part-only sequence (2204bp)

tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggct tcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggata acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacga gcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaag acacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac ggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctca gtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc taaagtatatatgagtaaacttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaa aaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactca ccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataa ggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccatt acgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaatta caaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttt tcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtct gaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgca cctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaa tatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagatt ttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagctcgagtgccacctgacgtctaagaaaccattattatcatga cattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttct agag

BioBricks from iGEM Parts Registry Distributions

[Test use] J04450 Part-only sequence (1069bp)

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaat taatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca tactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtca cgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgg gacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttca aatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcg tggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaa ggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtg cttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgc ttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

[Used] K314103 Part-only sequence (1638bp)

acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgcttt cttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgac cccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtg gactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatga gctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcg cagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggc ggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccg tcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcgg cggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgt tccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgca ttgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaa ttcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgct ggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgatacc gaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagg gccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggc cgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggat aacaagatactgagcacatactagagaaagaggagaaa

[Test use] R0010 Part-only sequence (200bp)

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaat taatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca

[Not used] R0011 Part-only sequence (55bp)

aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca

[Not used] K206000 Part-only sequence (130bp)

acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctac tgtttctccataccgtttttttgggctagc

[Test use] B0034 Part-only sequence (12bp)

aaagaggagaaa

[Test use] K518012 Part-only sequence (828bp)

tcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttc gaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcc tgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatggga acgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtacc aacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaa tcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaa aaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa cgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagcca gattattaatccggcttttttattattt

[Not used] B0012 Part-only sequence (41bp)

tcacactggctcaccttcgggtgggcctttctgcgtttata

Full table

No.	ID	Name / Function	Source	State / Concentration / Date Made	Gene Size / Sequence	Initial Plasmid / Vector	Comments
1	pAzoR / BBa_K1336000	FMN-dependent NADH-azoreductase 1	Pseudomonas putida	Miniprep, 48 ng/uL, 15/07/17, TO.	597 bp [Check! Not 612 bp?], Genomic Sequence	Expression vector pET-21a (+) (ampicillin resistant (ampR)), initially cloned between NdeI and BamHI.	Plasmid provided by Lisbon; with Paper.
2	p1B6 (AzoR 1B6) / BBa_K1336001	Mutant: Heat-stable; FMN-dependent NADH-azoreductase 1	Pseudomonas putida	Miniprep, 68 ng/uL, 15/07/14, TO.	597 bp [Check! Not 612 bp?], Genomic Sequence[Add sequence! Sent from Lisbon, see DT]	Expression vector pET-21a (+) (ampR), initially cloned between NdeI and BamHI.	Plasmid provided by Lisbon; with Paper.
3	pCotA / BBa_K1336002	Spore Coat Protein Laccase	Bacillus subtilis	Miniprep, 103 ng/uL, 15/07/14, TO.	1733 bp [Check! Not 1539 bp?] Genomic Sequence[Add sequence! Sent from Lisbon, see DT]	Expression vector pET-21a (+) (ampR), initially cloned between *NheI* and BamHI.	Plasmid provided by Lisbon; with Paper.
4	LiP / BBa_K1336003	Lignin Peroxidase	Phanerochaete chrysosporium (White-Rot Fungi)	[Being synthesised by Gene Oracle], X ng/uL, dd/mm/yy, Gene Oracle.	X bp, Genomic Sequence	[Cloned directly into expression vector, pSB1C3, between EcoRI and PstI.]	Synthesised (for free) by Gene Oracle. Sequence from Paper.
5	pBsDyP / BBa_K1336004	Dye Decolourising Peroxidase BSU38260	Bacillus subtilis	Miniprep, 51 ng/uL, 15/07/14, TO.	1251 bp, Genomic Sequence[Add sequence! Sent from Lisbon, see DT]	Expression vector pET-21a (+) (ampR), initially cloned between NdeI and BamHI.	Plasmid provided by Lisbon; with Paper.
6	pPpDyP / BBa_K1336005	Dye Decolourising Peroxidase PP_3248	Pseudomonas putida	Miniprep, 55 ng/uL, 15/07/14, TO.	861 bp [Check! Not 864 bp?], Genomic Sequence[Add sequence! Sent from Lisbon, see DT]	Expression vector pET-21a (+) (ampR), initially cloned between NdeI and BamHI.	Plasmid provided by Lisbon; with Paper.
7	BBa_J04450	RFP Coding Device	Spring 2014 BioBrick Distribution. Plate 4, Well 4B. [Check! DT?].	(1) Miniprep, 333 ng/uL, 01/07/14, TO. (2) Miniprep, 38 ng/uL (NanoDrop, dodgy!) 01/07/14, TO.	1069 bp, Genomic Sequence[Add sequence! Made from combined BioBricks?]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR).	LacI-, and CAP-, sensitive; can fail if system contains LacI or CAP protein! RFP Coding Device contains: LacI (R0010), strong RBS (B0034), mRFP1 (E1010), and double terminator (B0015 = B0010+B0012).
8	BBa_R0010	Promoter (LacI regulated)	Spring 2014 BioBrick Distribution. Plate 3, Well 4G. [Check! DT?].	Miniprep, 329.1 ng/uL, 01/07/14, TO.	200 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map.	This part is an inverting regulator sensitive to LacI and CAP. In the absence of LacI and CAP proteins, this part promotes transcription; in their presence, the part inhibits transcription. LacI can be inhibited by IPTG.
9	BBa_R0011	Promoter (LacI regulated, lambda pL hybrid)	Spring 2014 BioBrick Distribution. Plate 2, Well 6D (Inconsistent sequencing!). [Check! DT? / Maybe use Spring 2013 Distribution, Plate 5, Well 6G.].	Miniprep, 38 ng/uL (NanoDrop, dodgy!), 01/07/14, TO.	55 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map.	Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1.
10	BBa_K314103	Lac induced expression cassette	Spring 2014 BioBrick Distribution. Plate 1, Well 4D. [Check! DT?].	Miniprep, 334 ng/uL, 01/07/14, TO.	1638 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map.	Lactose (IPTG) inducible protein expression insert includes f1 origin (K314110), a Lac I generator (K314111), a lactose inducible promoter (R0011), and the Elowitz standard RBS (B0034).
11	BBa_K206000	pBAD Strong Promoter	Spring 2014 BioBrick Distribution. Plate 3, Well 14A. [Check! DT?].	Miniprep, 144 ng/uL, 01/07/14, TO.	130 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map	pBAD is an E. coli promoter that is induced by L-arabinose. In the absence of arabinose, the repressor protein AraC (BBa_I13458) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription; in its presence, transcription is permitted.
12	BBa_B0034	RBS	Spring 2014 BioBrick Distribution. Plate 4, Well 1N. [Check! DT?].	Miniprep, 156.5 ng/uL, 01/07/14, TO.	12 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1A2, i.e. ampicillin resistant (ampR). Plasmid / Vector Map	RBS based on Elowitz (1999) repressilator.
13	BBa_K518012	RBS + RFP + double Terminator	Spring 2014 BioBrick Distribution. Plate 1, Well 18C. [Check! DT?].	(1) Miniprep, 49 ng/uL, 01/07/14, TO. (2) Miniprep, 219.2 ng/uL, 08/08/14, YKH.	828 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map	This Coding Device contains: RBS.3 (medium) (B0032), mRFP1 (E1010), and double terminator (B0014 = B0012+B0011).
14	[CHECK: BAD PART !] BBa_B0012 (2)	Transcription Terminator for E. coli RNA polymerase	Spring 2014 BioBrick Distribution. Plate 2, Well 2B. [Check! DT?].	Miniprep, 128 ng/uL, 01/07/14, TO.	41 bp, Genomic Sequence[Add sequence!]	Plasmid Backbone: pSB1C3, i.e. chloramphenicol resistant (camR). Plasmid / Vector Map	TE from coliphage T7. This is a bad terminator (Experience: Fails). It is a promoter in the reverse direction.
#	ID	Name / Function	Source	State / Concentration / Date Made	Gene Size / Sequence	Initial Plasmid / Vector	Comments