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Team:Bielefeld-CeBiTec/Notebook/Journal/rMFC/Jun
From 2014.igem.org
June |
 |
- Initial cultivation experiments with neutral red
- Cultivation of the E. coli strain with porines (attempt 1) and wildtyp as control (attempt 2) in LB-medium with 100 µM neutral red
- pH was set up at 8.75
- Results
- Attempt 1 turned red (pH = 7.495)
- Attempt 2 remained yellow
- Reduction of neutral red
- Reducing agent: sodium dithionite
- Red solution got reduced and turned colorless
- Got oxidised by air again afterwards
- Oxidizing agent: hydrogen peroxide
- Red solution stayed red
- Yellow solution stayed yellow
- Conclusion: the color of the solution depends on the pH
