Team:TU Eindhoven/Plamid Design

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Project Description

Plasmid Design

Plasmid Design

The chosen vector for expression is pET29a. At both anchors at the N- and C-terminus the restriction sites for NdeI and SacI were introduced to clone the genes into a pET29a vector. The modified genes were first digested with the restriction enzymes and so was the pET29a vector. (Figure 1 and 2) After digestion the genes were ligated into the vector.



Figure 1. plasmid design pET29a(+) COMPx


Figure 2. Plasmid design pET29a(+) COMPy