Team:Evry/Interlab Study/08-23-2014

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Picture

Contruction n°1: PSB1C3 with I20260


Digestion of PSB1C3 and PSB1K3-I20260 by EcoRI and PstI
  • 1 µl EcoRI-HF
  • 1 µl PstI
  • 2 µl buffer
  • 0.2 µl BSA
  • 13.7 or 76 µl respectively for I20260 and PSB1C3
  • 10.8 µl miliQ watter

Incubation 45 minutes at 37°C. Denaturation step 20 minutes at 80 °C.
Loading on a 1% agarose gel to perform an extraction from gel

Contruction n°2: PSB1C3 with J23101-E1010


Aug 23