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Team:Evry/Interlab Study/08-23-2014
From 2014.igem.org
Contruction n°1: PSB1C3 with I20260
Digestion of PSB1C3 and PSB1K3-I20260 by EcoRI and PstI
- 1 µl EcoRI-HF
- 1 µl PstI
- 2 µl buffer
- 0.2 µl BSA
- 13.7 or 76 µl respectively for I20260 and PSB1C3
- 10.8 µl miliQ watter
Incubation 45 minutes at 37°C. Denaturation step 20 minutes at 80 °C.
Loading on a 1% agarose gel to perform an extraction from gel
Contruction n°2: PSB1C3 with J23101-E1010
Aug 23
