Team:Evry/Interlab Study/09-01-2014

Construction n°1: PSB1C3 with I20260

Construction n°2: PSB1C3 with J23101-E1010

Construction n°3: PSB1C3 with K823012-E1010

Constructions with other promoters

PCR colony were performed on colonies from transformations of the 29th August. Used protocol was the same as Table 1 and 2 with the Q5 high fidelity enzyme.
PCR product of first colony of each transformation was loaded on 1% agarose gel and gel run 45 minutes at 110 mV. 3 ml LB culture were started from tested colonies which presented the right PCR product profile. Cultures were incubated overnight at 37°C. Sep 01