Team:Evry/Interlab Study/09-01-2014
From 2014.igem.org
![Picture](https://static.igem.org/mediawiki/2013/9/90/EvryBiology.png)
Other constructions of the Anderson library of constitutive promoters
PCR colony were performed on colonies from transformations of the 29th August. Used protocol was the same as Table 1 and 2 with the Q5 high fidelity enzyme.
PCR product of first colony of each transformation was loaded on 1% agarose gel and gel run 45 minutes at 110 mV. 3 ml LB culture were started from tested colonies which presented the right PCR product profile. Cultures were incubated overnight at 37°C. Sep 01