agrBCDA Reception System and Combinatorial Promoters
Tuesday, 9/9/14
Export Systems Preparation of fsr system for LC/MS analysis:
Made new LB-CARB plates, since the old stock had run out.
Transformed pTG005-unFLAG into DH5α. Cells were plated & incubated overnight.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
Wednesday, 9/10/14
Export Systems Preparation of fsr system for LC/MS analysis:
Set up a liquid culture of pTG005-unFLAG cells.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
Thursday, 9/11/14
Export Systems Preparation of fsr system for LC/MS analysis:
Set up 0 nM, 250 nM, & 500 nM aTc inductions of pTG005-unFLAG expressing cells in MOPS media. 5 μL aliquots of last night's liquid culture was used to start each culture.
Made a glycerol stock of pTG005-noFLAG
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
Friday, 9/12/14
Export Systems Preparation of fsr system for LC/MS analysis:
aTc-induced MOPS liquid cultures from yesterday were spun down, and then the supernatant was loaded into C18 columns, washed with 10% acetonitrile, and then eluted with 60% acetonitrile.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
Saturday, 9/13/14
Export Systems Preparation of fsr system for LC/MS analysis:
Vacufuged pTG005-unFLAG acetonitrile samples to completion.
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