Team:Caltech/week12

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Notebook
Overview

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week Twelve

Sunday, 8/31/14

 Export Systems
Redo of cloning of agr system:
  • Ran colony PCR on 10 picked overnight colonies.
  • colony PCR products were then run on a gel.
  • 5 LB-CARB liquid cultures were set up and incubated overnight.
De-FLAGging pTG005 for LC/MS analysis:
  • Ran colony PCR on 10 picked overnight colonies.
  • colony PCR products were then run on a gel.
  • 3 LB-CARB liquid cultures were set up and incubated overnight.

Monday, 9/1/14

 Export Systems
Redo of cloning of agr system:
  • Miniprepped the 5 liquid cultures from yesterday. Stored the plasmids in the fridge.
De-FLAGging pTG005 for LC/MS analysis:
  • Miniprepped the 3 liquid cultures from yesterday. Stored the plasmids in the fridge.
lamBCDA & fsrABC Reception Systems

Tuesday, 9/2/14

 lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
  • Ran colony PCR using liquid cultures of Gibson colonies inoculated on Friday, in hopes of obtaining clearer gel results

Wednesday, 9/3/14

 Export Systems
  • Shipped samples for both agr-recloning subproject (2*5=10 samples) & pTG005-unFLAGging subproject (2*3=6 samples) out for sequencing.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
  • Ran gel of colony PCR products from yesterday
  • Gel still yielded unfavorable results
  • Planning to clone using Golden Gate assembly

Thursday, 9/4/14

 Export Systems
  • Sequencing data appears to confirm successful unFLAGging of pTG005.

Friday, 9/5/14

Saturday, 9/6/14

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