More paper-reading to determine what's wrong with our new quorum-sensing system candidates
Designed geneblocks for our new systems and subgroup projects
Tuesday, 7/1/14
Lab cleanup. Reorganized -20 freezer samples.
World Cup watching. Tim Howard's a boss
Work on the team wiki while we wait for our next batch of geneblocks to ship
Wednesday, 7/2/14
To figure out what went wrong during assembly of the combinatorial promoter constructs used in last week's TXTL workshop, we attempted to take out the LacI and TetR genes present in plasmids pAA001.
PCR extraction of linear fragments of pAA001 minus the lacI and tetR genes.
Recircularization of the truncated "backbone"+"combinatorial promoter construct" round-the-horn ligation
Transformation of recircularized plasmids into JM109 competent cells
Cells plated onto carbenicillin plates and incubated at 37°C overnight
In other news, we continued to work on the wiki and make it worse and worse......
Thursday, 7/3/14
Colony PCR of colonies that grew up last night
Due to the long weekend, we decided that we will wait until next week to begin minipreps
Friday, 7/4/14
It's the 4th of July!!!
'Murica...or something like that, I guess...
"
