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Team:Caltech/week10
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Revision as of 00:30, 15 September 2014 by
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Home
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Notebook
Overview
Week 1
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
Week 11
Week 12
Week Ten
Monday, 8/18/14
Export Systems
Preparation of fsr system for LC/MS analysis:
started vacufuging pAA001 acetonitrile samples (negative controls).
Colony PCR was run on 8 more colonies picked from pTG005-noFLAG plate (RTH reaction run last week).
Ran the PCR products on a gel.
Set up 6 LB-CARB liquid cultures corresponding to the promising colonies, as determined by the colPCR
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
Performed another QuickLigation reaction using yesterday's round-the-horn PCR product.
Ligation reaction product was transformed into JM109.
Picked six colonies from yesterday's transformations (RTH) and restreaked on a new plate & started overnight liquid cultures.
Tuesday, 8/19/14
Export Systems
The 6 liquid cultures prepared yesterday were miniprepped and shipped for sequencing.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
Performed colony PCR on the round-the-horn colonies obtained from Sunday's & Monday's transformations.
Sent promising colPCR products for sequencing.
Wednesday, 8/20/14
Preparation of our powerpoint presentations for SURF seminar day (see Thursday's entry).
Thursday, 8/21/14
Today is Summer Seminar Day (for SURF, our official summer research program)! All of us gave ~20-minute presentations on our individual subprojects.
Friday, 8/22/14
Export Systems
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
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