Team:CityU HK/notebook/lablog/project1

Week 3 (13/7~19/7)

- Primer design for leaderless tesA for Fit-coli (‘tesA) & New biobrick (‘tesA BB)

Week 4 (20/7~26/7)

- PCR of ‘tesA and ‘tesA BB

- PCR purification of ‘tesA and ‘tesA BB

- Prepare LB agar plates(‘tesA BB)

Week 5 (27/7~31/7)

- Digestion of ‘tesA BB and pSB1C3 plasmid with EcoRI & PstI restriction enzymes

- PCR purification for digested ‘tesA BB

- Sub-cloning of ‘tesA BB into pSB1C3 plasmid

- Transformation of ‘tesA BB into W3110 E.coli

- Pick colonies of the transformed E. coli with ‘tesA BB

Week 1 (1/8~2/8)

- Cell lysis of picked E. coli with ‘tesA BB

- Colonies PCR of ‘tesA BB by using VF2 & VR primer

- Send the ‘tesA BB plasmid for sequencing

Week 2 (3/8~9/8)

- 2¬nd PCR of ‘tesA
(∵ The first PCR product of ‘tesA was degraded over a long period of storage time)

- PCR purification of ‘tesA and ‘tesA BB

- Prepare LB agar plates(‘tesA BB)

Week 5 (27/7~31/7)

- Digestion of ‘tesA BB and pSB1C3 plasmid with EcoRI & PstI restriction enzymes

- PCR purification for digested ‘tesA BB

- Sub-cloning of ‘tesA BB into pSB1C3 plasmid

- Transformation of ‘tesA BB into W3110 E.coli

- Pick colonies of the transformed E. coli with ‘tesA BB

The bedding was hardly able to cover it and seemed ready to slide off any moment. His many legs, pitifully thin compared with the size of the rest of him, waved about helplessly as he looked. "What's happened to me?"

He thought. It wasn't a dream. His room, a proper human room although a little too small, lay peacefully between its four familiar walls.