Team:TU Eindhoven/Project/protocols

For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.

To create such resilient bacteria the 2014 team has designed a ‘plug and play’ system using copper free click chemistry to attach different chemical groups to create bio-layers on E. Coli cell membranes. Circularly permuted OmpX (CPX), an outer membrane protein, was mutated to contain an azido-functionalized unnatural amino acid. CPX functions as an anchor for any DBCO functionalized molecule to click onto. The polymers used in this project were designed to form hydrogels, which enables the bacteria to have antifouling properties.

Here will follow all genetic engineering protocols

Here will follow all genetic engineering protocols