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Team:TU Eindhoven/Notebook
From 2014.igem.org
Notebook
January
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec eu pulvinar odio, in eleifend sem. Mauris augue risus, suscipit eget commodo vitae, vestibulum ac arcu. Sed hendrerit lacus sit amet diam rutrum, ut hendrerit leo euismod. Phasellus vitae aliquet urna. Nullam pellentesque tempor diam at facilisis. Proin euismod, nisl vitae dictum lacinia, velit nibh semper eros, egestas rhoncus erat erat vel justo. Nam id dapibus orci.
February
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec eu pulvinar odio, in eleifend sem. Mauris augue risus, suscipit eget commodo vitae, vestibulum ac arcu. Sed hendrerit lacus sit amet diam rutrum, ut hendrerit leo euismod. Phasellus vitae aliquet urna. Nullam pellentesque tempor diam at facilisis. Proin euismod, nisl vitae dictum lacinia, velit nibh semper eros, egestas rhoncus erat erat vel justo. Nam id dapibus orci.
March
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec eu pulvinar odio, in eleifend sem. Mauris augue risus, suscipit eget commodo vitae, vestibulum ac arcu. Sed hendrerit lacus sit amet diam rutrum, ut hendrerit leo euismod. Phasellus vitae aliquet urna. Nullam pellentesque tempor diam at facilisis. Proin euismod, nisl vitae dictum lacinia, velit nibh semper eros, egestas rhoncus erat erat vel justo. Nam id dapibus orci.
April
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec eu pulvinar odio, in eleifend sem. Mauris augue risus, suscipit eget commodo vitae, vestibulum ac arcu. Sed hendrerit lacus sit amet diam rutrum, ut hendrerit leo euismod. Phasellus vitae aliquet urna. Nullam pellentesque tempor diam at facilisis. Proin euismod, nisl vitae dictum lacinia, velit nibh semper eros, egestas rhoncus erat erat vel justo. Nam id dapibus orci.
May
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec eu pulvinar odio, in eleifend sem. Mauris augue risus, suscipit eget commodo vitae, vestibulum ac arcu. Sed hendrerit lacus sit amet diam rutrum, ut hendrerit leo euismod. Phasellus vitae aliquet urna. Nullam pellentesque tempor diam at facilisis. Proin euismod, nisl vitae dictum lacinia, velit nibh semper eros, egestas rhoncus erat erat vel justo. Nam id dapibus orci.
June
Wetlab
- Arrival of the biobricks
- The designs for the plasmids are approved and ordered Primers
- Preparation of culture media, agar plates, antibiotics and glycerol
- Amplification of PET-29a(+), pEVOL-pAzF, BBa_K811005(Penn) & CPX
July
Wetlab
Week 27: 30 June – 6 July
Plasmid amplification and culturing BBa_K811005(Penn) + CPX
Vector and insert Digestions
BBa_K811005(Penn) + CPX insert ligate pET29a
Colony PCR
Week 28: 7 July – 13 July
Colony PCR
Site directed mutagenesis of CPX
Week 29: 14 July – 20 July
Site directed mutagenesis of CPX
Sequencing of CPX and BBa_K811005(Penn)
Week 30: 21 July – 27 July
Sequencing results came in and were as expected
Protein expression
FACS – DBCO-PEG4-5/6-TAMRA
August
Wetlab
Week 32: 4 August – 10 August
Protein expression
FACS – Antibody titration
Protein expression curve
Week 33: 11 August – 17 August
FACS - DBCO PEG-5kDa fluorescent
Week 34: 18 August – 24 August
Start Bio-bricking
Start silencing mutagenesis
Week 35: 25 August – 31 August
September
Wetlab
Week 36: 1 September – 7 September
Week 37: 8 September – 14 September
Week 38: 15 September – 21 September
Week 39: 22 September – 28 September
October
Wetlab
Week 40: 29 September – 5 October
Week 41: 6 October – 12 October
Week 42: 13 October – 19 October
