Open Source DIY Hardware
Being in the measurement track and having a team of highly motivated engineering and computer science students, we tackled the challenge to build, document and evaluate our open source hardware approach.
For our daily tasks in the lab, two key devices were detected: fluorometer and OD-meter. As we use GFP most of the time, the fluorometer is designed to work best with GFP. For modularity reasons, and re-usability, it is designed such that a change to another fluorescence protein is easy.
Besides the mandatory $\mu$-controller architecture, we worked together with the Fablab Aachen to construct the device. There we have the chance to use laser cutters and 3D printers.
The core component for detecting the light intensity is the cuvette holder. Please find the 3D model we printed below:
This cuvette holder can be used for both devices: the whole in the bottom is used for fluorescence measurement, the two opposite wholes are used for the light sensor and the LED for optical density measurement respectively.
Hardware Requirements
The key ingredients for our combined optical density/fluorescence (OD/F) device then are:
- Arduino UNO R3 (or equivalent)
- breadboard
- bluetooth Modem optional
- cuvette holder [2014.igem.org/Template:Team:Aachen/cuvette.stl?action=raw stl file download]
- TSL 235 R light to frequency sensor
- LED for optical density (for 600nm we recommend: DIALIGHT - 550-2505F )
- LED for fluorescence (any 450nm blue works for iLOV, 480nm for wtGFP )
The case is from acrylic glass. The construction plan can be downloaded from [2014.igem.org/Team:Aachen insert link here].
Fluorescence
Also general information
Characteristic Curve
Optical Density
Some general information
calibration
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